nanopore~split-barcode

Fast and accurate de novo assembler for single molecule sequencing reads

input_1:FASTQ

input_1/_pass_edb489fd_0.fastq

@e1bfba8b-0317-4528-82a3-0db1b0a9ed2b runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=5 ch=370 start_time=2020-10-29T06:59:49Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GTTGTGCTTCGTTCAGTTACGCGTATTGCTTGGGGATGACGTCAAATCATCATGCCCCGCGCCTTGATACTGGGCTACGTGCTTACAATGGACAGAACAAGGGCAGCGAAACCGCGAGGTCAAGCCAATCCCACAAATCTGTTCTCAGTTCGGATCGCAGTCTGCTCGACGCGTGAGCTGGGAATCGCTGTAATCATGAATCAGCATGCCGCGGTGGACCGTTCCAGGCGCTACACACCGTCACACCACGAGGATTTGTAACACCCGAAGTCAGTGAGGTAACCTTTGGAGCCAGCCGCCGAAGGTGGGACAGATGATTGGGGTGAAGTCGT
+
$&($%#*2/5774=(5'*+.3$%1&132222,)0>9&)7//<@B>;11)/0.A@ID8+('-4456.&###03?A@D)348*$%''(((%)7,,+2/0,,+*+-++43+8:+74.-'#*('#)3146:6//6/(.6962==AK@BAE-30:70@?57-))*;91+,/>>1-(''5.,36638*1869646,/--17'''$#'>B>9>@B@<5=232,''&'(9;&8'03)$#%$).&675625/08B=8:?5')#/21/+:1/-,('+-%1/7%&33243;9..,-.+&'()DJK7HI>93-7=5A>:?A956>ECCAAC8<7/<./452<'#
@1feabd1e-dbbe-4aff-9e8e-3ed765a09ec4 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=7 ch=351 start_time=2020-10-29T06:59:49Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GGTATTACTTCGTTCAGTTACGTATTGCTAGGTCTACCTCGCTAACACCACTGGTCGGTAAAACTCGTGCCAGCCACCGCGGTTATACGAGAGGCTGATTAATACCCCCGGCGTAAGGGTGGTTAAGAAAAATTTTTACTAGGGCTAAATATTCTCCAGCTGTCATACGCACCTGAGAAAAAAGAACCTTATGCAGAAGTGGCCTTAACTTATCTGAATCCACGAGCCAGGAACAAACTGGGATTAGATGCCCCCACTATGATGAGTTCTC
+
$#/&'))@A>><D?C3E475@@H=BC9:32$',)),5318004444BJM8::473A4DDDC/AA>>1>9M;F<<@;AC8599H;733--,*+,*''&%012=88B9KI21=3445/&7200'.#50)9=4210H768ADB19814873D?@THHB)>6&00-,9-:8><=>@><6.(9D@*%$%'(()(*/&#%%%44'>-41///34''(67=>5+<58-.''&&-+&969EAIB';:=A2/9;..:10B@ML>,$$;:=?>>;>9A?1)
@babc0869-b961-47a8-a6a1-3b971517e69c runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=5 ch=315 start_time=2020-10-29T06:59:48Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GATGTACTTCGTTCAGTTACGTATTGCTTTGCGTCCTGTTGAGAACTCATCATAGTGGGGTATCTAATCCCAGTTTGTTCCACAACATTGGATTCAGATAGAAGTTAAGGCCACTTTCGTATAAGGTTCTCTTTTCTCAGGTGCGTATGACGGCTTGGAGAATGTTTAGCCCTAGTAAAAATTTTCTTAACCACCCTTTACGCCGGGGAGTATTAGCGGGCCTCTCGTATAACCGCGGTGGCTGGCACGGTTACCGACCAAGTGGTGTTATGAGGAGTAGACCCTGGCAATACGTAACT

input_1/_pass_edb489fd_1.fastq

@34e5ce05-39e6-43f1-970b-9075248a4c20 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=437 ch=220 start_time=2020-10-29T07:05:52Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CAGAGTATCTTCGTTCAGTTACGTATTGCTTCAGAACGACTTCCATACTCGTGTGAGTCAGGTAAGCTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGTGTTTAGATCACCCCCTCCCAATAGGCTAAAACTCACCTGAGTTGTAAAGCTCAGTTGGCTAATAAGACTACGAAGTGGCTTTAACATATCTGAACACACAATGGCTAAGGTGAAGCTGGGATTAGATACCCCACTATGATGAAGGTTCTCGTAACCGGACGCATTGCGTCCTGTTGCGAAAGAGCAGTGAACATCTGAATCTTGCGTGACCGAATAGCTGTTAAAGCCCTTTCGCTACGTGTCGGCGAAGTTTACAACTCAGGCCTAGCGTGAGAAGCGATCTAAAACACGCCGGCTGTGACGGAGCCGCGATGCTTCGACTCACCACAAT
+
,+%$$&#$#%48:>8G,2-/.//@./2/00:/'(254>=<<<+9)/03(>:=740%)'0#$%$)%%+*'#'%.223;=2@CB43/,;*(+*/<=BA<::6BD:8AA<993;,@D?4>7;*-@1-.57;3,30+9.3433**6@@-555%'3328>>@:>700--+->*/8;5+,4(<7;:?)('$'+,2$42;=2%%%%+:=<8985+.23:20=9??2-)$%48934526/,'$(%&&%+,+764<<.1*''.3.*'(8;<=>=><-52GG(79/03/%$.3255677;068../2495&,,+*%))&''&''$$')(*&&%#$#$%%#&%)$%**'()+#$&')*012398&$'13$""#"%$&/3$&%%&(&7@8%,2(0/5)*)%#$$%%),1+'$%$"(05;9463().)**+)'&%#%$$#$+%$351/,&&&%(*+)+*5<5((&&&##
@a9691b25-d0ae-4089-91f2-15077e8daffb runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=370 ch=74 start_time=2020-10-29T07:05:52Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GTTGCGCTTCGTTTCAGTTACGTATTGCTTGCGTCCTGTTACGAACTCATCAGTGGTATCTAATCCCGGTTGTTCCTGGCAGCTTTCGTGGATTCAGATAAGTTAGGCCACTTTCGTATAAGGTTCTCTTTTTCTCAGGTGCGTATGACAGCTTGGAGAATATTTAGCCCTAGTAAAAATTTTTCTTAACCACCCTTTACGCCGGGGGTATTAGCGGGCCTCGTATAACGCGGTGGCTGGCACGAGTTTACCGACCAGTGGTGTTAGCAGGTAGACCTGGCAATACGTAAC
+
##%%#$#$&$/(@7=0:29$**3/14-(/-4,/66;>BDA+,1,*:??30.&$0/2:64>@>84491*(?>7E>5,8*'&(+-:<09%>(9(E9A>>3//.,+.(**;:...7646D526%0/A;B>DBB@?6;5D+.:>@2659:7'$$%%%%+()(93///08-<@AC:%-=II@@43><;?569<=FI@HIK56,0201/$17679323'$$%(.*(/(#%%&8+..)73:1C=>=)(%$+$5.2*05<:5IJ?BB;381--+'%$(56&'%4;,%114--/1/)&%$
@a0f377a4-1b3f-41a1-950e-0b0ac84f9d53 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=355 ch=183 start_time=2020-10-29T07:05:49Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CGATGTGCTTCGTTCGGTTATTATTGCTGATGTAGAGGGTACGGTTTGAGGCAGAAGTTTGATCCTGGCTCAGGATGAACCGCTGGCGGTATGCTTAACACATACAAGTCGTACGAAGATCTTTGATCTTAGTGGCGGACAGGTGAGTAACACGTGAAAATCTGCCCTTAGGAAGAGGATAACGGCTGAAACGGTTGCTAATACTCTATACGCCGAGGTAAAAGTTATCCACCTTAGGAAGAGCTCGCGTCTGATTAACGTTGGTAGGTATTGAAAACACCAAGGCGACGATCAGTAGCTGGTTTGAAGGACGACCAGCCTGGGACTGAGACACGGCGAACTCCTACGGGTAGCAGTGAAGTACGCAATGGGCGAAAGCCTGACGAGCAATACAACGTGAGGGATGAAAGCCTGTGGGTTGTAAACCTCTTTTCTCAGGAAGAAGTTCTGACGGTACTTGAGGAATAAGCATCAGCTAACTCTATTGTAGCTAGCCACTTGGTAATACAGAGGATGCAAGCGTTATCCCATCACTGGGCATAAAGCGTCTGTAGGTCGCTTACCAAGTCTGCTGTTAAAGATCGGGGCTAACCCTGGGAAAGCAGTGGAAACTAGTAGGCTTGAGTATGGTGAGGTGGGAATTCCTGGTGTAGCGGTGAAATGCGTAGATGTGGAAGAACACCAATGGCGAAAGCACTCTACTGGGCCGCAGCTGACACTGAGAGACGACAACAGGGAGCAAATGGGATTAGATACCCCAGTAGTCCCTGGCCGTAAACGATGGATACTAGGTGTTGCACGTATTAACCCGTGCAGTATCGTAGCTAAAGGCGTTAAGTATCCCGCCTGGGAAGTACCTT

input_1/_pass_edb489fd_2.fastq

@5d186b72-4236-4b2d-b6d2-bfee9f0d8589 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=789 ch=463 start_time=2020-10-29T07:11:33Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CATTGTACTTCGTTCAGTTACGTATTGCTAGGTCTACCTCGCTAACACCACTGGTCGGTAAAACTCGTGCCAGCCACCGCGGATTATACGAGAGGCCAAGTTAATACCCCGGCGTAAGGGTGGTTAAGAAAAGTACACTAGGGCTAAATATTCTCAAGCGTTGTCATACGCACCTGAGAGAAAGAGAACCTTATACGAAAGTGGCCTTAACTTATCTGAATCCACGAAAGCCAGGAACAAACTGGGATTAGATACCCCCACTATGATGAGTTCTCAT
+
%#$%)?8,,@@B?8?/:/0335@A?@62(,52/'5%'')69>BC,8BKFCC@048<-2<:?A;-1DBBCK;D+>@@CC0--כ:89;-2&+'%%&&#-50,,?<=34?8AC;8'29DBENG@<27701%7&%/440356;6BA8;=C;<*C@57422=<>@:?@779@GICB54:'5;0(.-)7.,-.53444::53<(53.01/.*,.255444C:;=1+-.0.7@E16946?=@;=?BCA>C<@:=77D?NOH9BD??@9@?:<5LH42$#
@6e818f48-e7a7-4610-8c73-9f69246e6d17 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=843 ch=307 start_time=2020-10-29T07:11:33Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CGGTATTAGTTCGTTCAGTTACGTATTGCAGAACGACTTCCCATGCTCGTGTGAGTCGGTAAAAACTCGTGCCAGCCACCGCGGTTATACGAGAGGCCCAAGTTGATGAACTCCGGCGTAAAGAGTGGTTAAGACAAATTTTAAACTAAAGCCGAATGCCACTCCAGGACTGTTATACGCTCCCGAAGGTAAGCCCAATCACGAAAGTGATGGCTTTTATATGAGGCTGAGCCCACGGCTTATGATACAAGCGCTGGGATTAGATGCCACACATATGATCTCTTGACACT
+
1%%2%$%%$&0.2B>E1F14&--0*()%%234@=D?MPCA@5'&&021+8$334.(2$9@C?@HC@;3&*--19?GD<=9+%+%<$;3322441./)(%024395/%%$&&'-40;)/765),2.969E;?@=@DE=C8JCGFJ9ABHD?:?=-.3=<><$##%%%)&%)7<BD/)):=AC5A%2293<9;.$-,'>;761,./0058-%%,D>>?:0-&&#&'#%*&&$&--*$"$-))+1214676<-*.1==-27-29317'&%&'())',.*+)&2-445,)%%%%
@c6f546df-08f5-4855-925d-98f353480aa4 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=991 ch=414 start_time=2020-10-29T07:11:33Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CTTGTACTTCGTTCAGTTACGTATTGCTAGGTCTACCTCGCTAACACCACTGGTCAGTAACTCGTGCCAGCCACCGCGGTTATACGAGGGCCCAAGTTAACAGAACCTCGGCGTAAGCGATGGTTAGATAACAATAAACTAAAAAATGAATACTCTCTCGAGGCTGTTATACGCACCACCTGAAGGCTAGAAGCCCACTACGAAAGTGGCTTTTTACCTAATTTGAACCCACAGCTAAGGCACAAACTGGGATTAGATGCCCACATGATGAGTTCTCGTAA

input_1/_pass_edb489fd_3.fastq

@510ddd62-c082-471e-9fd0-f55e6496a637 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=979 ch=463 start_time=2020-10-29T07:13:48Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CGGTATTGCTTCGTTCAGTTACGTATTGCTAGAACGACTTCCATGCTCGTGTGAGTCGGTAAAACTCGTGCCAGCCACCGCGGTCACACGGTCAACCCAAGTCAATAGAAGCCGGCGTAAAGTGTTTTAGATCACCCTCCCCAATACAAAGCTAAAACTCACCTGAGTTTGTAAAACTCCAGTTGACACAAATAGACTACGAAAGTGGCTTTGCTTATATCTGAACACACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGATGAGTTCTCAT
+
.$(($$($DF99<A8D+F+,2==G668;;87/4)'/7;:5BB-%%$(6;?938<.).&1+39BC@=?67;?AA1EH:CD@:942>50622&*(*&%'%642.@?<77&)&)=@@@8>BB7('3*6>==>462;176>,=II*>6513;;3(*.',,;:14,35685:0A21;?9=A@B@HD@OL;4/0009,''';))1357104>7A@@A/%%%'7B62C;::G@?:AAD-**+'((**517(2A8?79=G?:?<;976A<P:5DG>883))&$38+1.+%
@0e84b1f1-9b93-411f-b985-3f85f0096965 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=979 ch=466 start_time=2020-10-29T07:13:48Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GATACTTCGTTCAGTTACGTATTGCTCCCAAACCCAACAACCTAGATAGGCTAAACCAACAACCTAGATAGGCTACACTTTCAGTATAACCCTCAGGAGAGTACCTCGATACCCTCCCAGGAAAGTACCTCTGATCACACCTTCCAGTACACTTACCCTCCAGGAAAGTACCTCTGATACCCTCAGGAAAGTACCTCTGGGTTGCACTTTCCAGTACACTTAGGAAACTTGCATGTGTGAAATTTCCATTCCCTCCGATAGATAAACTCCATTCCTCAAATAGATGAAACAACAATGTAAAAA
+
(#$$&&+&'//7++15013:)*.$#$0+%=A??92=-+C97:7,-1+,+189>66&('./:2''$'-*((,.13/*+,.4)80..(29;=4$&1)0+%($&)(2/10++,->>2)%*%%8%3-$++8=<00).+*2*%)++*/'2&(*%'13B@;437%';217,1#/49>73=2=-))@B;+4&'%('%.6255-/))'.3+&(+%%2>(B=.9&)****$%1)<'555,'&'+)/')&%&'9;0A<E??;;(+5/3011&$(63:70B@>?16'/)&%&&,.)&-.),0*&.+'&&%+,+(
@10494a25-3989-4e29-a329-412d6c538c7e runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=1295 ch=235 start_time=2020-10-29T07:17:27Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CAGCGTAACTGTTCGATTACGTATTGCTAGGTCTACCTCGCTAACACCACTGGTCGGTAAAACTCGTGCCAGCCACCGCGGTTATACGAGAGGCCCAAGTTAATACCCCGGCGTAAAGGGTGGTTAGAAAAATTTTACTGAAACAAATATTCTCCAAGCTGTCATACGCACCTGAGAAAAGAGAACCTTATGCAAAGTGGCCTTAACTTATCTGAATCCACAGCCAGGAACAAACTGGGATTAGATACTTACTATGATGAGTTCTCGTAA

input_1/_pass_edb489fd_4.fastq

@14bebf45-a60f-492b-bba4-f30ff6e73068 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=1989 ch=8 start_time=2020-10-29T07:22:49Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CTTGTACTTCGTTCAGTTACGTGTGCTCCAAACCCAGCAACCTAGATAGGCCGACAACAACCTAGATAGGCTACACCTTCAGTACACTTACCATGTACGACTTGGTTACTCCCTTTGCCTGAGTTAAATTTTAGGAAAGTTTGTTTTGTGGCTTGGGGAGAGTGACGAGCTTGGTGTGTGTGCGCGCTTTTGGTCAGTTTCGGTAAAACGCTCTGTTTTTTTACCTACTGCTAAATCCTCCTTCAATTGATTTTCACTTCAATTTCGTTTTCTCTGCGGGAGAGAATCATATGTATTTTTGCCCCTCACGGCTTCACCTCGACCTGACGTTTTGAGTTATACCTTAATTTTGCTTACTTTAGATCCTTCACAGATGTGTAAGCTGACGGCGGCGGTATATAGGCGGAATAAACAAGAGGAGGGATAAGCAGGGTTATCGGTTCTTAAACAGGCTCCTCTAGGGGTCTAAAGCACCGCCAAGTCCTTTGGAGTTTTAAGCCCCTTTGATGCTCATGTAGTACCCTGGCATTAGGCAGTAAATTATGCTAATAAGGTTTAAGGCAAGACATAGTGGGAGTATCTAAACCCAGTTTGTTCCCTGGCTTTCGTGGATTCAGATAAGTTAAGGCCACTTTCGTATAAAGGTTCTCTTTTTTCTCAGGTGCGTACTTGACAACGGAGAATATTTAGCCCTAGTAAAAATTTTTTCTTGCCACCTTTACGCCGGGGTATTAGCGGGCCTCTCGTATAACCGCGGTGGCTGGCACGAGTTTAACAGGCCCTGTTGACATAACTAAGTCGAAGTTTTCACTTATGGCTTAAGATTTATCACTGCTGAATTCCCTTGGGGGTGTGGCTAAAGCAAGGCGTTTTGGGCTAAAAAGTAGAGTATGATGCCAGCTCACTTGCTCCGGGCCGGGGAGTGTTAAGGGCATTCTCTGGGCGTGCGGAAACTTGCATGTATAAATTCCATTCCCTCCGATAGATGAAACTCCATTCCCTCCGT
+
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
@58780695-4d18-43bc-8dca-373d978633a8 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=1714 ch=449 start_time=2020-10-29T07:22:50Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
CGGTATTGCTTCGTTCAGTTACGTATTGCTCCAAACCCAACAACCTAGATAATTTAAACCCAACAACCTGGATAGGCTACACTTTCCGGTACATACCCGCATCCAATATTTCGCGAGCATGCGAAGGGTTATGCAGATTTTTTCTGCTGTCTACCTTGATACAACATTTCGTTGTGCCACCAGTTCGTTTTGTTCACAACACCAGTGTGGTATCCGGCATCAGCGAAATCCTTAGTTTCTACGTACTTCTTACGCTTCTGTACATCCACGGCGCTTTCGCTGCTTTGACCCGGCGCGAAGCGTAGCTGCCGTGGTCTACAGCGCTCAAATGCAGTAAAGCAAATTTCTGAGTGATGCCTTCGATACCGTAGTAACAAGTAATGTTGTATCAGGCGAAAAAAAATCTGCATAACAATATTGAATAGTAAAATGCTAACTCAGCAATTGTTGGACTGAGCTGTCAAC
+
-%()(()&LJGCA;292806*345%)55>>5B3/*:B5<(3<5(&&-)(&&%$&&(+*1:.:(88*875%*,))5;;4)'&3>CE8C46

input_1/_pass_edb489fd_5.fastq

@dda963f0-686a-46ff-b45a-0cae57a280b2 runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=2181 ch=223 start_time=2020-10-29T07:28:45Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GGTGTACTTCGTTCAGTTACGTATTGCTTTGCGTCCTGTTACGAGAACTCATCACATGTTGGGGTATCTAATCCCAGTTTGTTTCTCAACTGTCGTGAGTTCAAAGGTGTTAAAGCCACTTTCGTAGTGGGGCTTCGTTTTATCCGGTAGCGTATGACAGCTTGGGAAGTGTTTGGCACGTTTGTTTTGTTTTTAATCACGCTTTACGCCGTGTAATATCAATTTGAGCCCCTCGTATAACCGCGGTGGCTGGCACGCGAGTTTTACCGACTCTATGGGTCCCAAGAGACTCGTTAGCAATACGTAAC
+
$$&'2'3><.7;1E:B::;<=FFGC911=.4;?@AJ5EFFA55-23DBBD1/)($*%##&(%(+8<?GDDB?AE7.?9F>.;GDG;=D;43-0:=FE=?95&),+)045<61522=;-*?CH>>@HC@;<CBEG5B@KJIEA9:9B@;>AD8C77:7229===8BK7A:7<:D>>-''$%%)//@HJH7<@KGGF?;>-2989=422//+.<-&>:=<5;(/'0.<<BD>A(0?4?=7C5??0.047,(:<2+'&#''3.42DEE@DHAA@1:8++:A?697--1*0<><=B=45+(,4122'*2*($
@5517177a-4632-4e24-85eb-bbc0dad1995a runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=2344 ch=182 start_time=2020-10-29T07:28:45Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GTTGTACTTCGTTCAGTTACGTATTGCTAGGTCTACCTCGCTAACACCACTGGTCGGTAAAACTCGTGCCAGCCACCGCGGTTATACGAGAGGCCCAAGTTAATACCCCGGCGTAAAGGGTGGTTAAGAAAAATTTTTACTAGGGCTAAATATTCTCAAGCTGTCATACGCACCTGAAGAGAGCCTTATACGAAAGTGGCCTTAACTTATCTGGAATCCACGAAAGCCGGGAACAAACTGATTAGATACCCCACTATGATGAGTTTATAA
+
).'/586@.;98+>17268??G?<;(#))+87?70447<=@=>.+?HGCHCB63;05EBC8*;:D=B@?A?***+0+1((++;-677974+6>=>18175D6419A6K:36$7?DE'034;:>M@F6.>H;>A:ICCB<CF<7)(,0%&%75:A=(9-)&%*(/81('(+.07754/(*($,#(*():66553.*/6<AA<;8*')*+/'&$()(322='572892@1%+:-:690'++,&'>=<40/(1'%')13?<@953>$:1#$$$
@399307dc-752f-4d69-bac6-3b290a4673fa runid=edb489fddf22cf262c8a8b162d91ab00ca2ad5c1 read=2255 ch=115 start_time=2020-10-29T07:28:45Z flow_cell_id= protocol_group_id=student-experiment sample_id=eDNA_metagenome
GTTGTACTTCGTTCAGTTACGTATTGCTAGAACGACTTCCATACTCGTGTTAGTCGGTAAAACTCGTGCCAGCCACCGCAGTCACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAACTCCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGCTAAGACCCAGAAGCTGGGATTAGATGCCTACTATGATGAGTTCTCGTA

input_2:FASTA

input_2/primers.fa

>12S-F-BC09
AACTAGGCACAGCGAGTCTTGGTTAACTAGGCACAGCGAGTCTTGGTTAHTTAYACATGCAAGTHTCM
>12S-F-BC10
AAGCGTTGAAACCTTTGTCCTCTCAAGCGTTGAAACCTTTGTCCTCTCAHTTAYACATGCAAGTHTCM
>12S-F-BC11
GTTTCATCTATCGGAGGGAATGGAGTTTCATCTATCGGAGGGAATGGAAHTTAYACATGCAAGTHTCM
>12S-F-BC12
CAGGTAGAAAGAAGCAGAATCGGACAGGTAGAAAGAAGCAGAATCGGAAHTTAYACATGCAAGTHTCM
>12S-R-BC17
ACCCTCCAGGAAAGTACCTCTGATACCCTCCAGGAAAGTACCTCTGATYRCACYTTCCRGTACRCTTA

input_3:table

input_3/sample.txt

16S-food-1	27F-BC01	1492R	1300	1800
16S-food-2	27F-BC03	1492R	1300	1800
16S-food-3	27F-BC05	1492R	1300	1800
16S-food-4	27F-BC07	1492R	1300	1800
16S-food-5	27F-BC89	1492R	1300	1800
16S-food-6	27F-BC91	1492R	1300	1800
16S-eDNA-aquarium-1	27F-BC02	1492R	1300	1800
16S-eDNA-aquarium-2	27F-BC04	1492R	1300	1800
16S-eDNA-aquarium-3	27F-BC06	1492R	1300	1800
16S-eDNA-sanshiro-1	27F-BC08	1492R	1300	1800

Option

-c 15 -m 60 input_1/ input_2/primers.fa input_3/sample.txt

Output

16S-eDNA-sanshiro-1.fq

@d2f94e7c-8678-4b29-8155-eff6219c2f45:29:1501:-1:27F-BC08:1492R
TTCAGGAACAAACCAAGTTACGTAGAGTTTGATCCCCGGCTCAGAACGAACGTTGGCGGCGTGCCTAACACATGCAAGTCGAGCGGGGAGCAATCCCTAGCGGCGGACGGGTGCGTAACACGAGTAACCTGCCCTGAGGCTGGGATAACGTTCCGAAAGGGGCGCTAATACCGGATAGTTCTTGTTGTGGGATGCCATGACAGAGGAAAGGAGCAATCCACCTGAGGAGGGCTCGCGGCCCATTAGCTAGTTGGGCGGTAATGGCGCACCGCGAAGATGGGTAGCCGGTCTGAGAGGATGATCGGCCACACTGAACTGAGACATTTGTCCAGACTACGGGAGGCAGCAGTGGGGAAATATTGCGCAATGGAGGAAACTCTGACGCAGCAACGCCGCGTGGGTGAAGCATATTCCTCGGATTGTAAAAGCCCTGTCGGGAGGGAAGAAAAGCACATGGTGAATGTGTCTGACGGTACCTTCTTGATGAAGCACTGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGAGTGCAAAACGTTGTTCGGAATTATTGCTGCAAGGAGCGGGCAGGCGGGCAGGTGTGTCAGGCGTGAAACCTGCGGCTCAACCCTGGGCCAGCTTTGAAACTACCTGTCTAGAGATTCGGAGGGTGTTTTGGAATTCGTGGAGCAGTGAAACGCGTAGATATCACGAGGAACACCGACGGCGAAGGCAGCTTACTGACAAGATCTGACGCTCAGGCTCGAAAGCGTGGGGAGCAAAAGGATTAGATACCCTGTAGTCCACGCTGTAAACGGTGCGCATTAGGTCTCGGAGCTTTTCGAACGCTTCGGGGCTCGGGTTAACGCATTAAATGCGCCGCCTGAGGACTACGGCCGCACGCTAAAACCAAAGGAATTGACGGGGCCCACAGCGGTGGAGCATGTGGCTTAATCTGATGAACGCGAAGAACCTACCTTAGGTTTGACATGACGTGGTACCCGGGCGAAAGCTTTGGGGACCCGCAAGGAGCGTTCACAGGTGCTGCATGGCTGTCGTCGCTCGCCGTGAGATGTTCGTTAAGTCCGGCAACGAGCGCAACCCCGCCTCCAGTTACCAGCGAGTGCAAAGTCGGGGACGGAGGGATCTAACCCGTTAGGGTGGGAAGGTGGGGATGACGTCAAGTCAGTATGGCCCTTGAGATCTAGCCGCACACGTGCTACAATGCCTGGCACAAAAGGAAATAATACCGCGAGGTGGAGGAAACCCGGAAAGCCAGGCCCAGTTCAGATTGAAGTCTGCAACTCGAATTTCATGAAGCCGGAATCGCTAGTAATGGTAGATCAGCTTACGCTACCGTGAATACGTTCCCGGGCCTTGTACACACCGCCCGTCACGTCACATGAGTCGCTCAATCCCGAAGTATCCGAGCCAACCGCAAGGGGTGGGTCCTATGAATGGTGGTGACGACGAAGTCGTAACAAGGTAACC
+
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