##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename output.sickle.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3379 Sequences flagged as poor quality 0 Sequence length 5001-309422 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 19.002959455460196 18.0 16.0 21.0 15.0 25.0 2 16.975140574134358 16.0 12.0 22.0 8.0 27.0 3 17.38650488310151 17.0 11.0 23.0 7.0 29.0 4 16.270494229061853 15.0 9.0 22.0 6.0 28.0 5 16.792838117786328 16.0 9.0 24.0 5.0 30.0 6 16.86149748446286 16.0 8.0 24.0 5.0 31.0 7 15.978691920686593 14.0 8.0 23.0 5.0 30.0 8 14.218999704054454 12.0 7.0 20.0 5.0 27.0 9 13.39893459603433 11.0 7.0 18.0 4.0 27.0 10-4999 18.660357056225582 17.49438877755511 11.636673346693387 25.00681362725451 7.8084168336673345 31.823246492985973 5000-9999 18.76014902975363 17.6618 11.7688 25.111 7.8914 31.9048 10000-14999 18.753434420266995 17.6166 11.7082 25.1246 7.8352 31.856 15000-19999 18.74390649198132 17.5708 11.6568 25.1084 7.7562 31.8416 20000-24999 18.744830510931177 17.561 11.629 25.1098 7.7072 31.834 25000-29999 18.746741603402686 17.5578 11.6228 25.0818 7.7182 31.7886 30000-34999 18.686046575721242 17.4908 11.5264 25.0156 7.6084 31.7382 35000-39999 18.705269431642904 17.4856 11.5526 25.0314 7.621 31.7278 40000-44999 18.751981746829326 17.5448 11.603 25.058 7.6548 31.7002 45000-49999 18.738629816187828 17.5264 11.5442 25.0216 7.6056 31.6652 50000-54999 18.74202037457657 17.517 11.5824 24.9646 7.6248 31.6064 55000-59999 18.69013729505495 17.4516 11.4866 24.9056 7.5336 31.5774 60000-64999 18.834761181248975 17.5932 11.624 25.0392 7.647 31.5554 65000-69999 18.869988638182193 17.6186 11.665 24.995 7.6286 31.513 70000-74999 18.73281528025792 17.438743706545193 11.496523615439942 24.819467753536323 7.5058738911532 31.315511867657637 75000-79999 18.787522168869476 NaN NaN NaN NaN NaN 80000-84999 18.712001749693663 NaN NaN NaN NaN NaN 85000-89999 18.73328931401302 NaN NaN NaN NaN NaN 90000-94999 18.668768663439003 NaN NaN NaN NaN NaN 95000-99999 18.626510844336067 NaN NaN NaN NaN NaN 100000-104999 18.725316814977717 NaN NaN NaN NaN NaN 105000-109999 18.665854503327694 NaN NaN NaN NaN NaN 110000-114999 18.44524786324788 NaN NaN NaN NaN NaN 115000-119999 18.52277489480079 NaN NaN NaN NaN NaN 120000-124999 18.56859663476869 NaN NaN NaN NaN NaN 125000-129999 18.80540795493632 NaN NaN NaN NaN NaN 130000-134999 18.302736190476153 NaN NaN NaN NaN NaN 135000-139999 18.962470879120897 NaN NaN NaN NaN NaN 140000-144999 19.23531666666668 NaN NaN NaN NaN NaN 145000-149999 18.743560606060676 NaN NaN NaN NaN NaN 150000-154999 18.635054545454544 NaN NaN NaN NaN NaN 155000-159999 18.83015515151516 NaN NaN NaN NaN NaN 160000-164999 18.546066666666647 NaN NaN NaN NaN NaN 165000-169999 18.732711111111126 NaN NaN NaN NaN NaN 170000-174999 18.72397777777777 NaN NaN NaN NaN NaN 175000-179999 18.212386904761903 NaN NaN NaN NaN NaN 180000-184999 18.76005238095242 NaN NaN NaN NaN NaN 185000-189999 18.68043333333333 NaN NaN NaN NaN NaN 190000-194999 18.529400000000017 NaN NaN NaN NaN NaN 195000-199999 19.276429999999966 NaN NaN NaN NaN NaN 200000-204999 18.5446 NaN NaN NaN NaN NaN 205000-209999 18.29745 NaN NaN NaN NaN NaN 210000-214999 18.5402 NaN NaN NaN NaN NaN 215000-219999 17.97395 NaN NaN NaN NaN NaN 220000-224999 17.894466666666602 NaN NaN NaN NaN NaN 225000-229999 19.1329 NaN NaN NaN NaN NaN 230000-234999 17.5226 NaN NaN NaN NaN NaN 235000-239999 16.909 NaN NaN NaN NaN NaN 240000-244999 18.5736 NaN NaN NaN NaN NaN 245000-249999 19.1982 NaN NaN NaN NaN NaN 250000-254999 19.4758 NaN NaN NaN NaN NaN 255000-259999 18.2504 NaN NaN NaN NaN NaN 260000-264999 18.4782 NaN NaN NaN NaN NaN 265000-269999 19.1322 NaN NaN NaN NaN NaN 270000-274999 20.0004 NaN NaN NaN NaN NaN 275000-279999 19.763 NaN NaN NaN NaN NaN 280000-284999 18.2274 NaN NaN NaN NaN NaN 285000-289999 18.695 NaN NaN NaN NaN NaN 290000-294999 19.6412 NaN NaN NaN NaN NaN 295000-299999 19.4996 NaN NaN NaN NaN NaN 300000-304999 18.7612 NaN NaN NaN NaN NaN 305000-309422 19.385032783178836 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores fail #Quality Count 14 2.0 15 40.0 16 255.0 17 618.0 18 991.0 19 1024.0 20 411.0 21 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.295649600473514 5.534181710565256 55.04587155963303 20.124297129328202 2 21.041728321988753 8.967150044391833 55.1938443326428 14.797277300976619 3 21.870375850843445 12.577685705830127 35.217519976324354 30.334418467002074 4 30.68955312222551 12.814442142645754 32.376442734536845 24.11956200059189 5 28.174015981059487 10.298905001479728 45.30926309559041 16.217815921870375 6 19.26605504587156 10.180526783071915 49.15655519384433 21.396862977212194 7 23.11334714412548 11.571470849363717 37.11157147084936 28.20361053566144 8 23.2909144717372 16.86889612311335 32.11009174311927 27.730097662030182 9 29.47617638354543 18.55578573542468 31.9621189701095 20.00591891092039 10-4999 24.848946190694498 25.197100326726257 25.384459359678218 24.569494122901027 5000-9999 24.8541681804595 25.432499459667657 25.139264436062525 24.574067923810322 10000-14999 24.87018993609638 25.408660850690573 25.031488178815465 24.689661034397577 15000-19999 24.829155472304084 25.33570322844414 25.12258408286099 24.712557216390792 20000-24999 24.936054769130443 25.205838207618704 25.12046244913883 24.73764457411202 25000-29999 25.0869022930397 25.149088813453595 24.93971469286501 24.824294200641695 30000-34999 25.117217526530723 25.042608056693517 25.026314961658798 24.813859455116962 35000-39999 25.064854479977548 25.15212942686884 25.094411466955624 24.688604626197986 40000-44999 25.049277169410345 25.28579572998363 24.925773115229756 24.739153985376266 45000-49999 25.21604175697688 25.2042058791764 24.946035794653437 24.633716569193282 50000-54999 25.30632111025774 25.224069873363458 24.755211031132227 24.714397985246574 55000-59999 25.125670735698215 25.138438989741406 25.13125014082633 24.604640133734048 60000-64999 25.01260735648949 25.158464773106378 25.209799342607177 24.619128527796956 65000-69999 24.964569083660617 25.447427380263772 25.011498482200352 24.576505053875263 70000-74999 25.060206508323553 25.200186640175804 25.194354134681955 24.545252716818688 75000-79999 25.083988810415057 25.514975216050896 25.2218509215346 24.179185051999447 80000-84999 25.272759524410322 25.265264414969234 24.800817466603043 24.661158594017405 85000-89999 24.95598401215768 25.439078783984975 25.112897147763984 24.492040056093355 90000-94999 25.525263313817053 24.720704145194862 24.978365778169316 24.775666762818762 95000-99999 25.469295724833106 25.214642557142223 24.64520977938901 24.67085193863566 100000-104999 25.373932455935417 25.107598058302834 24.859434623471692 24.65903486229006 105000-109999 25.24898320180875 25.006072761019727 25.21348090661651 24.53146313055502 110000-114999 25.590312520541342 24.446570600135846 24.76281597148679 25.20030090783602 115000-119999 25.263461697039663 25.164508946977822 24.83796487177373 24.73406448420879 120000-124999 24.94059170660224 25.438631232920116 24.760386549962373 24.860390510515266 125000-129999 24.52336680302467 26.253873806867485 25.301847031114416 23.92091235899343 130000-134999 24.808988764044944 26.02655771195097 25.25161729656112 23.91283622744297 135000-139999 25.13464433717736 25.33641683093017 24.805599962749696 24.72333886914278 140000-144999 24.748333333333335 25.44 25.161666666666665 24.65 145000-149999 23.889580041020814 25.654819945529738 25.727110722571535 24.72848929087791 150000-154999 24.672727272727276 24.976363636363637 24.754545454545454 25.596363636363634 155000-159999 25.665653753798424 24.08253469049491 24.77315710066087 25.478654455045795 160000-164999 24.946666666666665 24.173333333333332 25.037777777777777 25.842222222222222 165000-169999 25.626666666666665 24.831111111111113 24.66 24.88222222222222 170000-174999 24.90888888888889 25.2 24.244444444444444 25.64666666666667 175000-179999 25.226586102719033 25.311727547377096 24.696511947267236 24.76517440263664 180000-184999 25.55940853179796 25.30751112274274 24.195236848992412 24.937843496466893 185000-189999 26.066666666666666 23.823333333333334 25.093333333333334 25.016666666666666 190000-194999 26.106666666666666 24.093333333333334 24.560000000000002 25.240000000000002 195000-199999 25.135124774792043 24.87062521562464 25.95545674090543 24.038793268677885 200000-204999 24.955 25.319999999999997 25.069999999999997 24.654999999999998 205000-209999 24.815 24.495 25.595000000000002 25.095 210000-214999 22.955000000000002 25.995 27.345000000000002 23.705000000000002 215000-219999 24.79 24.245 26.075 24.89 220000-224999 22.97244094488189 25.96456692913386 27.034120734908136 24.028871391076116 225000-229999 24.768152001809547 25.356254241121917 26.24971725853879 23.625876498529745 230000-234999 25.480000000000004 25.740000000000002 24.7 24.08 235000-239999 27.3 23.44 25.080000000000002 24.18 240000-244999 26.66 24.560000000000002 22.74 26.040000000000003 245000-249999 24.62 26.56 25.52 23.3 250000-254999 23.14 28.22 28.04 20.599999999999998 255000-259999 22.7 27.58 29.68 20.04 260000-264999 21.6 28.08 29.099999999999998 21.22 265000-269999 22.24 28.28 27.12 22.36 270000-274999 22.5 28.9 28.060000000000002 20.54 275000-279999 21.66 25.7 28.82 23.82 280000-284999 24.58 25.540000000000003 27.279999999999998 22.6 285000-289999 24.86 26.740000000000002 26.22 22.18 290000-294999 25.779999999999998 25.34 24.7 24.18 295000-299999 26.58 23.56 23.880000000000003 25.979999999999997 300000-304999 26.86 22.32 24.740000000000002 26.08 305000-309422 27.76396111236717 23.85258874067375 24.93782500565227 23.445625141306806 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0196078431372549 37 0.14304951045548925 38 0.21874884127615027 39 0.2544216282413869 40 0.3995944389662297 41 0.33970122827612675 42 0.55688697895816 43 0.8624343085638843 44 1.2960489301417262 45 1.930060460486944 46 2.279690628019266 47 1.5460936014793607 48 2.4734446459684727 49 3.0349155802486214 50 3.964294659379437 51 4.07851869011828 52 2.9114624428845928 53 1.6096524190180677 54 0.7161504256129358 55 1.0985756092369698 56 2.0506889291375687 57 0.7948472323497244 58 0.01639344262295082 59 0.0 60 0.0 61 0.0 62 0.0 63 0.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-4999 0.0 5000-9999 0.0 10000-14999 0.0 15000-19999 0.0 20000-24999 0.0 25000-29999 0.0 30000-34999 0.0 35000-39999 0.0 40000-44999 0.0 45000-49999 0.0 50000-54999 0.0 55000-59999 0.0 60000-64999 0.0 65000-69999 0.0 70000-74999 0.0 75000-79999 0.0 80000-84999 0.0 85000-89999 0.0 90000-94999 0.0 95000-99999 0.0 100000-104999 0.0 105000-109999 0.0 110000-114999 0.0 115000-119999 0.0 120000-124999 0.0 125000-129999 0.0 130000-134999 0.0 135000-139999 0.0 140000-144999 0.0 145000-149999 0.0 150000-154999 0.0 155000-159999 0.0 160000-164999 0.0 165000-169999 0.0 170000-174999 0.0 175000-179999 0.0 180000-184999 0.0 185000-189999 0.0 190000-194999 0.0 195000-199999 0.0 200000-204999 0.0 205000-209999 0.0 210000-214999 0.0 215000-219999 0.0 220000-224999 0.0 225000-229999 0.0 230000-234999 0.0 235000-239999 0.0 240000-244999 0.0 245000-249999 0.0 250000-254999 0.0 255000-259999 0.0 260000-264999 0.0 265000-269999 0.0 270000-274999 0.0 275000-279999 0.0 280000-284999 0.0 285000-289999 0.0 290000-294999 0.0 295000-299999 0.0 300000-304999 0.0 305000-309422 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-9999 1413.0 10000-19999 1032.0 20000-29999 386.0 30000-39999 169.0 40000-49999 134.0 50000-59999 77.0 60000-69999 48.0 70000-79999 34.0 80000-89999 30.0 90000-99999 12.0 100000-109999 15.0 110000-119999 7.0 120000-129999 7.0 130000-139999 3.0 140000-149999 1.0 150000-159999 2.0 160000-169999 0.0 170000-179999 2.0 180000-189999 1.0 190000-199999 2.0 200000-209999 0.0 210000-219999 0.0 220000-229999 3.0 230000-239999 0.0 240000-249999 0.0 250000-259999 0.0 260000-269999 0.0 270000-279999 0.0 280000-289999 0.0 290000-299999 0.0 300000-309423 1.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.88162178159219 #Duplication Level Percentage of deduplicated Percentage of total 1 99.88148148148149 99.76324356318437 2 0.11851851851851852 0.23675643681562594 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-4999 0.0 0.010301751772263518 0.03373423378274991 0.03688347396183649 0.0 5000-9999 0.004853506954720317 0.02959455460195402 0.05918910920390804 0.09096774193549068 0.0 10000-14999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.11837821840781608 0.0 15000-19999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.11939627108612486 0.0 20000-24999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 25000-29999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 30000-34999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 35000-39999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 40000-44999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 45000-49999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 50000-54999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 55000-59999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 60000-64999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 65000-69999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 70000-74999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 75000-79999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 80000-84999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 85000-89999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 90000-94999 0.02959455460195402 0.02959455460195402 0.05918910920390804 0.14797277300976913 0.0 95000-99999 0.053039360757624374 0.02959455460195402 0.05918910920390804 0.17141757916545008 0.0 100000-104999 0.05918910920390804 0.02959455460195402 0.05918910920390804 0.17756732761173738 0.0 105000-109999 0.05918910920390804 0.02959455460195402 0.06778336786032128 0.18616158626812498 0.0 110000-114999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 115000-119999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 120000-124999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 125000-129999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 130000-134999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 135000-139999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 140000-144999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 145000-149999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 150000-154999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.2071618822136612 0.0 155000-159999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23605800532702628 0.0 160000-164999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 165000-169999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 170000-174999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 175000-179999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 180000-184999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 185000-189999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 190000-194999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 195000-199999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 200000-204999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 205000-209999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 210000-214999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 215000-219999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 220000-224999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 225000-229999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 230000-234999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 235000-239999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 240000-244999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 245000-249999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 250000-254999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 255000-259999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 260000-264999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 265000-269999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 270000-274999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 275000-279999 0.05918910920390804 0.02959455460195402 0.08878366380586869 0.23675643681563216 0.0 280000-284999 0.05918910920390804 0.02959455460195402 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