[2019-09-06 08:54:36] root: INFO: Starting Flye 2.4.2-release [2019-09-06 08:54:36] root: DEBUG: Cmd: /root/miniconda2/bin/flye --nano-raw all.fastq --out-dir output --threads 8 --genome-size 10M [2019-09-06 08:54:36] root: INFO: >>>STAGE: configure [2019-09-06 08:54:36] root: INFO: Configuring run [2019-09-06 08:54:36] root: INFO: Total read length: 65381103 [2019-09-06 08:54:36] root: INFO: Input genome size: 10000000 [2019-09-06 08:54:36] root: INFO: Estimated coverage: 6 [2019-09-06 08:54:36] root: INFO: Reads N50/N90: 28370 / 8124 [2019-09-06 08:54:36] root: INFO: Minimum overlap set to 5000 [2019-09-06 08:54:36] root: INFO: Selected k-mer size: 15 [2019-09-06 08:54:36] root: INFO: >>>STAGE: assembly [2019-09-06 08:54:36] root: INFO: Assembling disjointigs [2019-09-06 08:54:36] root: DEBUG: -----Begin assembly log------ [2019-09-06 08:54:36] root: DEBUG: Running: flye-assemble -l /data/user2/work/84/output/flye.log -t 8 -v 5000 -k 15 all.fastq /data/user2/work/84/output/00-assembly/draft_assembly.fasta 10000000 /root/miniconda2/lib/python2.7/site-packages/flye/config/bin_cfg/asm_raw_reads.cfg [2019-09-06 08:54:36] DEBUG: Build date: Apr 7 2019 02:34:37 [2019-09-06 08:54:36] DEBUG: Total RAM: 204 Gb [2019-09-06 08:54:36] DEBUG: Available RAM: 199 Gb [2019-09-06 08:54:36] DEBUG: Total CPUs: 32 [2019-09-06 08:54:37] DEBUG: Parameters: [2019-09-06 08:54:37] DEBUG: big_genome_threshold=29000000 [2019-09-06 08:54:37] DEBUG: low_cutoff_warning=1 [2019-09-06 08:54:37] DEBUG: hard_min_coverage_rate=10 [2019-09-06 08:54:37] DEBUG: assemble_kmer_sample=1 [2019-09-06 08:54:37] DEBUG: repeat_graph_kmer_sample=1 [2019-09-06 08:54:37] DEBUG: read_align_kmer_sample=1 [2019-09-06 08:54:37] DEBUG: maximum_jump=1500 [2019-09-06 08:54:37] DEBUG: maximum_overhang=1500 [2019-09-06 08:54:37] DEBUG: repeat_kmer_rate=100 [2019-09-06 08:54:37] DEBUG: assemble_ovlp_divergence=0.30 [2019-09-06 08:54:37] DEBUG: repeat_graph_ovlp_divergence=0.15 [2019-09-06 08:54:37] DEBUG: repeat_graph_ovlp_end_adjust=0.00 [2019-09-06 08:54:37] DEBUG: read_align_ovlp_divergence=0.25 [2019-09-06 08:54:37] DEBUG: max_coverage_drop_rate=5 [2019-09-06 08:54:37] DEBUG: chimera_window=100 [2019-09-06 08:54:37] DEBUG: min_reads_in_disjointig=4 [2019-09-06 08:54:37] DEBUG: max_inner_reads=10 [2019-09-06 08:54:37] DEBUG: max_inner_fraction=0.25 [2019-09-06 08:54:37] DEBUG: add_unassembled_reads=0 [2019-09-06 08:54:37] DEBUG: max_separation=500 [2019-09-06 08:54:37] DEBUG: tip_length_threshold=100000 [2019-09-06 08:54:37] DEBUG: unique_edge_length=50000 [2019-09-06 08:54:37] DEBUG: min_repeat_res_support=0.51 [2019-09-06 08:54:37] DEBUG: out_paths_ratio=5 [2019-09-06 08:54:37] DEBUG: graph_cov_drop_rate=10 [2019-09-06 08:54:37] DEBUG: coverage_estimate_window=100 [2019-09-06 08:54:37] DEBUG: extend_contigs_with_repeats=1 [2019-09-06 08:54:37] DEBUG: Running with k-mer size: 15 [2019-09-06 08:54:37] DEBUG: Running with minimum overlap 5000 [2019-09-06 08:54:37] DEBUG: Metagenome mode: N [2019-09-06 08:54:37] INFO: Reading sequences [2019-09-06 08:54:37] DEBUG: Building positional index [2019-09-06 08:54:37] DEBUG: Total sequence: 65381103 bp [2019-09-06 08:54:37] DEBUG: Expected read coverage: 6 [2019-09-06 08:54:37] INFO: Generating solid k-mer index [2019-09-06 08:54:37] DEBUG: Hard threshold set to 2 [2019-09-06 08:54:37] DEBUG: Started k-mer counting [2019-09-06 08:54:55] INFO: Counting k-mers (1/2): [2019-09-06 08:54:57] INFO: Counting k-mers (2/2): [2019-09-06 08:55:00] WARNING: Unable to separate erroneous k-mers from solid k-mers. Possible reasons: (1) Incorrect expected assembly size parameter (2) Highly uneven coverage of the assembly (3) Running with error-corrected reads in raw reads mode Assembly will continue, but results might not be optimal [2019-09-06 08:55:00] DEBUG: Estimated minimum kmer coverage: 2 [2019-09-06 08:55:00] DEBUG: Filtered 0 erroneous k-mers [2019-09-06 08:55:00] DEBUG: Repetitive k-mer frequency: 441 [2019-09-06 08:55:00] DEBUG: Filtered 51 repetitive k-mers (6.05421e-06) [2019-09-06 08:55:00] INFO: Filling index table [2019-09-06 08:55:00] DEBUG: Sampling rate: 1 [2019-09-06 08:55:00] DEBUG: Solid k-mers: 8423837 [2019-09-06 08:55:00] DEBUG: K-mer index size: 37135807 [2019-09-06 08:55:00] DEBUG: Mean k-mer frequency: 4.40842 [2019-09-06 08:55:05] DEBUG: Sorting k-mer index [2019-09-06 08:55:05] DEBUG: Peak RAM usage: 1 Gb [2019-09-06 08:55:05] INFO: Extending reads [2019-09-06 08:55:05] DEBUG: Estimating overlap coverage [2019-09-06 08:55:43] INFO: Overlap-based coverage: 10 [2019-09-06 08:55:43] INFO: Median overlap divergence: 0.120416 [2019-09-06 08:55:43] DEBUG: Sequence divergence distribution: | * | ** | **** | ***** | ****** | ****** | ****** | ****** | ******* | ******* | ******** | ******** | ********* | ********* | ********** | ************ | ************ | ************* | **************** | ******************** * ---------------------------------------------------------------------------------------------------- 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% Q25 = 0.11, Q50 = 0.12, Q75 = 0.13 [2019-09-06 08:55:44] DEBUG: Assembled disjointig 1 With 10 reads Start read: +175e48b7-be6a-4ed1-a9da-a8ac8c932d23 At position: 7 leftTip: 1 rightTip: 1 Suspicious: 2 Mean extensions: 7 Avg overlap len: 10669 Min overlap len: 5839 Inner reads: 0 Length: 47285 [2019-09-06 08:55:44] DEBUG: Ovlp index size: 22690 [2019-09-06 08:55:44] DEBUG: Inner: 162 covered: 218 total: 6758 [2019-09-06 08:55:44] DEBUG: Discarded disjointig with 7 reads and 3 inner overlaps [2019-09-06 08:55:44] DEBUG: Assembled disjointig 2 With 5 reads Start read: +a81662b5-54ee-4a43-ae68-3da334936929 At position: 4 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 12 Avg overlap len: 10516 Min overlap len: 6226 Inner reads: 0 Length: 21085 [2019-09-06 08:55:44] DEBUG: Ovlp index size: 23368 [2019-09-06 08:55:44] DEBUG: Inner: 194 covered: 282 total: 6758 [2019-09-06 08:55:46] DEBUG: Assembled disjointig 3 With 178 reads Start read: +0285277d-6e69-4a61-9181-68e948758b07 At position: 2 leftTip: 1 rightTip: 1 Suspicious: 1 Mean extensions: 6 Avg overlap len: 31411 Min overlap len: 5393 Inner reads: 0 Length: 3595667 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 25903 [2019-09-06 08:55:46] DEBUG: Inner: 3132 covered: 3476 total: 6758 [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 220 reads and 175 inner overlaps [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 174 reads and 171 inner overlaps [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 71 reads and 61 inner overlaps [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 50 reads and 40 inner overlaps [2019-09-06 08:55:46] DEBUG: Assembled disjointig 4 With 47 reads Start read: +3c4673d1-1d87-4c6c-bfb2-cd780883fb76 At position: 18 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 6 Avg overlap len: 29895 Min overlap len: 8805 Inner reads: 0 Length: 1010689 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 25945 [2019-09-06 08:55:46] DEBUG: Inner: 3846 covered: 4216 total: 6758 [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 146 reads and 132 inner overlaps [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 202 reads and 196 inner overlaps [2019-09-06 08:55:46] DEBUG: Discarded disjointig with 214 reads and 197 inner overlaps [2019-09-06 08:55:46] DEBUG: Assembled disjointig 5 With 4 reads Start read: +8bfca72a-3330-4344-98d5-89417d09eac4 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 14 Avg overlap len: 10774 Min overlap len: 9024 Inner reads: 0 Length: 41039 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 26300 [2019-09-06 08:55:46] DEBUG: Inner: 3850 covered: 4250 total: 6758 [2019-09-06 08:55:46] DEBUG: Assembled disjointig 6 With 4 reads Start read: +a6e14ca4-adf9-4cb4-b6b1-34d8d20ef43c At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 14 Avg overlap len: 9793 Min overlap len: 9669 Inner reads: 0 Length: 36166 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 26330 [2019-09-06 08:55:46] DEBUG: Inner: 3864 covered: 4258 total: 6758 [2019-09-06 08:55:46] DEBUG: Assembled disjointig 7 With 5 reads Start read: +d04b2faa-52f8-4aa4-920a-bdc7d5d2cf36 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 15 Avg overlap len: 15782 Min overlap len: 5138 Inner reads: 0 Length: 35958 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 26716 [2019-09-06 08:55:46] DEBUG: Inner: 3872 covered: 4272 total: 6758 [2019-09-06 08:55:46] DEBUG: Assembled disjointig 8 With 6 reads Start read: +21109e95-0d0f-4a51-b05b-a37ab4bd52e5 At position: 4 leftTip: 0 rightTip: 0 Suspicious: 1 Mean extensions: 16 Avg overlap len: 13109 Min overlap len: 6936 Inner reads: 0 Length: 31709 [2019-09-06 08:55:46] DEBUG: Ovlp index size: 26806 [2019-09-06 08:55:46] DEBUG: Inner: 3882 covered: 4292 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 9 With 4 reads Start read: +34c8e8af-6609-426c-8af9-18225b64e6ef At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 11 Avg overlap len: 25478 Min overlap len: 21600 Inner reads: 0 Length: 100108 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 27053 [2019-09-06 08:55:47] DEBUG: Inner: 3906 covered: 4344 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 10 With 4 reads Start read: +702cd8cd-b789-4f9e-bbc9-9cd36493e521 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 15 Avg overlap len: 9304 Min overlap len: 9248 Inner reads: 0 Length: 41504 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 27173 [2019-09-06 08:55:47] DEBUG: Inner: 3912 covered: 4350 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 11 With 5 reads Start read: +7446e44c-c77c-497d-8c1f-21a07cc868df At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 23 Avg overlap len: 7786 Min overlap len: 5728 Inner reads: 0 Length: 28274 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 27383 [2019-09-06 08:55:47] DEBUG: Inner: 3922 covered: 4368 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 12 With 4 reads Start read: +5652a578-d24c-48db-b3b7-3ecc6c535fb1 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 12 Avg overlap len: 9440 Min overlap len: 9425 Inner reads: 0 Length: 23121 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 27833 [2019-09-06 08:55:47] DEBUG: Inner: 3928 covered: 4406 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 13 With 5 reads Start read: +9ec5fe11-542c-41b4-b640-08c5bc845490 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 24 Avg overlap len: 12661 Min overlap len: 5126 Inner reads: 0 Length: 24997 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 27953 [2019-09-06 08:55:47] DEBUG: Inner: 3934 covered: 4452 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 14 With 5 reads Start read: +4f32f557-5ea7-42a7-9a83-ebf3f83ae86e At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 7 Avg overlap len: 16623 Min overlap len: 8806 Inner reads: 0 Length: 106791 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 28077 [2019-09-06 08:55:47] DEBUG: Inner: 3948 covered: 4474 total: 6758 [2019-09-06 08:55:47] DEBUG: Assembled disjointig 15 With 4 reads Start read: +39e91498-20c9-4bdd-96f3-c91deab8a110 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 15 Avg overlap len: 9559 Min overlap len: 9498 Inner reads: 0 Length: 31955 [2019-09-06 08:55:47] DEBUG: Ovlp index size: 28201 [2019-09-06 08:55:47] DEBUG: Inner: 3952 covered: 4486 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 16 With 4 reads Start read: +47bd762d-bcc3-4d88-a115-571aacf16669 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 19 Avg overlap len: 6662 Min overlap len: 6660 Inner reads: 0 Length: 23261 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 28296 [2019-09-06 08:55:48] DEBUG: Inner: 3962 covered: 4504 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 17 With 4 reads Start read: +288cdcb6-93bb-463c-95f9-5aeaa1e439d9 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 11 Avg overlap len: 8380 Min overlap len: 8377 Inner reads: 0 Length: 16352 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 28326 [2019-09-06 08:55:48] DEBUG: Inner: 3970 covered: 4508 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 18 With 4 reads Start read: +0311a2f5-5194-4a24-8d5e-0019b319dec4 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 6967 Min overlap len: 6936 Inner reads: 0 Length: 25365 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 28626 [2019-09-06 08:55:48] DEBUG: Inner: 3974 covered: 4550 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 19 With 4 reads Start read: +2468d908-66db-4b0d-a446-77af7cbe3668 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 7710 Min overlap len: 7397 Inner reads: 0 Length: 28204 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 28656 [2019-09-06 08:55:48] DEBUG: Inner: 3978 covered: 4552 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 20 With 4 reads Start read: +4bf30f1c-5258-4a86-9f05-662e48ffb1ce At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5560 Min overlap len: 5558 Inner reads: 0 Length: 24312 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 28806 [2019-09-06 08:55:48] DEBUG: Inner: 3984 covered: 4570 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 21 With 4 reads Start read: +b45eab76-1dce-4247-ae90-e2403938b93c At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 23 Avg overlap len: 5243 Min overlap len: 5243 Inner reads: 0 Length: 26987 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 29094 [2019-09-06 08:55:48] DEBUG: Inner: 3988 covered: 4606 total: 6758 [2019-09-06 08:55:48] DEBUG: Assembled disjointig 22 With 4 reads Start read: +c409925c-09c4-4fea-81da-06695c878c49 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 17 Avg overlap len: 6850 Min overlap len: 6844 Inner reads: 0 Length: 18037 [2019-09-06 08:55:48] DEBUG: Ovlp index size: 29184 [2019-09-06 08:55:48] DEBUG: Inner: 3992 covered: 4614 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 23 With 4 reads Start read: +a13731d4-3bab-453a-88d0-9f6b942b8e52 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5818 Min overlap len: 5818 Inner reads: 0 Length: 23758 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29424 [2019-09-06 08:55:49] DEBUG: Inner: 3996 covered: 4646 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 24 With 4 reads Start read: +5a347215-9c67-4212-9c35-1304241e578d At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 19 Avg overlap len: 5169 Min overlap len: 5167 Inner reads: 0 Length: 15592 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29536 [2019-09-06 08:55:49] DEBUG: Inner: 4002 covered: 4658 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 25 With 4 reads Start read: +01e8578c-4525-42e0-8c05-d4152ee67abd At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 26 Avg overlap len: 5571 Min overlap len: 5521 Inner reads: 0 Length: 16899 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29596 [2019-09-06 08:55:49] DEBUG: Inner: 4008 covered: 4682 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 26 With 4 reads Start read: +3eed3673-61ce-4844-a51b-81d0cc1dc4fa At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 6 Avg overlap len: 19815 Min overlap len: 19259 Inner reads: 0 Length: 69361 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29656 [2019-09-06 08:55:49] DEBUG: Inner: 4028 covered: 4710 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 27 With 4 reads Start read: +c7972f46-964d-4a39-9f38-038b5a518fe9 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5661 Min overlap len: 5594 Inner reads: 0 Length: 14755 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29757 [2019-09-06 08:55:49] DEBUG: Inner: 4032 covered: 4736 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 28 With 4 reads Start read: +88b4f774-1a4d-4ffa-87c4-b3a20f6df566 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 8 Avg overlap len: 16821 Min overlap len: 12392 Inner reads: 0 Length: 105979 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 29814 [2019-09-06 08:55:49] DEBUG: Inner: 4058 covered: 4776 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 29 With 4 reads Start read: +c1316980-60fe-45c8-85f1-36292d822b65 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 8 Avg overlap len: 5065 Min overlap len: 5064 Inner reads: 0 Length: 23054 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 30112 [2019-09-06 08:55:49] DEBUG: Inner: 4062 covered: 4818 total: 6758 [2019-09-06 08:55:49] DEBUG: Assembled disjointig 30 With 4 reads Start read: +650c5575-10a3-43a1-ae1a-23d942fa6f12 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 21 Avg overlap len: 5063 Min overlap len: 5063 Inner reads: 0 Length: 23441 [2019-09-06 08:55:49] DEBUG: Ovlp index size: 30202 [2019-09-06 08:55:49] DEBUG: Inner: 4072 covered: 4864 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 31 With 4 reads Start read: +480378eb-1653-41ec-9d66-ec0c289722ae At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5865 Min overlap len: 5863 Inner reads: 0 Length: 35549 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30232 [2019-09-06 08:55:50] DEBUG: Inner: 4076 covered: 4882 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 32 With 4 reads Start read: +6e71a7f8-1d2f-4e39-9719-305bf7caca9f At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 21 Avg overlap len: 5074 Min overlap len: 5073 Inner reads: 0 Length: 20336 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30398 [2019-09-06 08:55:50] DEBUG: Inner: 4080 covered: 4912 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 33 With 5 reads Start read: +b0e957cc-903b-42ae-9acc-a7977a5d1b0f At position: 4 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 10 Avg overlap len: 21283 Min overlap len: 11955 Inner reads: 0 Length: 110871 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30500 [2019-09-06 08:55:50] DEBUG: Inner: 4102 covered: 4936 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 34 With 4 reads Start read: +0147863b-a34c-4654-94ec-ca2d2ad15b48 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 17 Avg overlap len: 7366 Min overlap len: 7332 Inner reads: 0 Length: 35674 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30560 [2019-09-06 08:55:50] DEBUG: Inner: 4108 covered: 4950 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 35 With 4 reads Start read: +f216b1e5-7f89-4207-ac40-dee93c76ede1 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 15 Avg overlap len: 7536 Min overlap len: 7536 Inner reads: 0 Length: 27316 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30730 [2019-09-06 08:55:50] DEBUG: Inner: 4112 covered: 4974 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 36 With 4 reads Start read: +f9a27226-4654-4e88-a0cd-4daa159b1c05 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 7735 Min overlap len: 7734 Inner reads: 0 Length: 27201 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30760 [2019-09-06 08:55:50] DEBUG: Inner: 4116 covered: 4978 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 37 With 4 reads Start read: +75db85e7-5e9d-4cc9-8a85-cd6f44309d93 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 10 Avg overlap len: 26534 Min overlap len: 6670 Inner reads: 0 Length: 160530 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30850 [2019-09-06 08:55:50] DEBUG: Inner: 4132 covered: 4996 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 38 With 4 reads Start read: +551f304b-4f95-4ada-a2a2-7f94b47009e6 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5366 Min overlap len: 5360 Inner reads: 0 Length: 18236 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 30850 [2019-09-06 08:55:50] DEBUG: Inner: 4140 covered: 5022 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 39 With 5 reads Start read: +af6aca42-be1e-4bb8-8e8b-5017b822bd93 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 10 Avg overlap len: 39272 Min overlap len: 7534 Inner reads: 0 Length: 82756 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 31029 [2019-09-06 08:55:50] DEBUG: Inner: 4166 covered: 5067 total: 6758 [2019-09-06 08:55:50] DEBUG: Assembled disjointig 40 With 4 reads Start read: +3fee9be0-732c-43d2-b078-6a678820bbc2 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 21 Avg overlap len: 6419 Min overlap len: 6419 Inner reads: 0 Length: 20199 [2019-09-06 08:55:50] DEBUG: Ovlp index size: 31059 [2019-09-06 08:55:50] DEBUG: Inner: 4170 covered: 5072 total: 6758 [2019-09-06 08:55:51] DEBUG: Assembled disjointig 41 With 4 reads Start read: +8092a799-4f33-491a-8e85-a0d7956755b1 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 18 Avg overlap len: 6126 Min overlap len: 6096 Inner reads: 0 Length: 19757 [2019-09-06 08:55:51] DEBUG: Ovlp index size: 31329 [2019-09-06 08:55:51] DEBUG: Inner: 4174 covered: 5120 total: 6758 [2019-09-06 08:55:51] DEBUG: Assembled disjointig 42 With 4 reads Start read: +4657388e-45df-40d3-baa0-b995a27f43c2 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 23 Avg overlap len: 5214 Min overlap len: 5152 Inner reads: 0 Length: 27864 [2019-09-06 08:55:51] DEBUG: Ovlp index size: 31492 [2019-09-06 08:55:51] DEBUG: Inner: 4182 covered: 5150 total: 6758 [2019-09-06 08:55:51] DEBUG: Assembled disjointig 43 With 4 reads Start read: +c947c0f8-88f8-42d2-964a-7d7764638d02 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 6630 Min overlap len: 6506 Inner reads: 0 Length: 23007 [2019-09-06 08:55:51] DEBUG: Ovlp index size: 31942 [2019-09-06 08:55:51] DEBUG: Inner: 4190 covered: 5228 total: 6758 [2019-09-06 08:55:51] DEBUG: Assembled disjointig 44 With 6 reads Start read: +36dd35e4-730a-41b2-b33f-81ecf271d8c5 At position: 4 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 7 Avg overlap len: 47491 Min overlap len: 7139 Inner reads: 0 Length: 119477 [2019-09-06 08:55:51] DEBUG: Ovlp index size: 32133 [2019-09-06 08:55:51] DEBUG: Inner: 4206 covered: 5254 total: 6758 [2019-09-06 08:55:52] DEBUG: Assembled disjointig 45 With 4 reads Start read: +31122ca1-6ffa-4a2b-94d4-71f69f051928 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5860 Min overlap len: 5860 Inner reads: 0 Length: 24947 [2019-09-06 08:55:52] DEBUG: Ovlp index size: 32267 [2019-09-06 08:55:52] DEBUG: Inner: 4210 covered: 5268 total: 6758 [2019-09-06 08:55:52] DEBUG: Assembled disjointig 46 With 4 reads Start read: +b5a34784-c18c-4a8f-ad25-c16c0f35f0d2 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 11 Avg overlap len: 6290 Min overlap len: 6197 Inner reads: 0 Length: 83853 [2019-09-06 08:55:52] DEBUG: Ovlp index size: 32341 [2019-09-06 08:55:52] DEBUG: Inner: 4228 covered: 5290 total: 6758 [2019-09-06 08:55:52] DEBUG: Assembled disjointig 47 With 5 reads Start read: +da148c8a-0446-4614-a666-7c5b33c6c078 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 25 Avg overlap len: 11990 Min overlap len: 5031 Inner reads: 0 Length: 19798 [2019-09-06 08:55:52] DEBUG: Ovlp index size: 32641 [2019-09-06 08:55:52] DEBUG: Inner: 4234 covered: 5362 total: 6758 [2019-09-06 08:55:53] DEBUG: Assembled disjointig 48 With 4 reads Start read: +c1f1a6a4-bdb0-4145-862f-f274883dbf41 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 7365 Min overlap len: 5207 Inner reads: 0 Length: 40230 [2019-09-06 08:55:53] DEBUG: Ovlp index size: 33151 [2019-09-06 08:55:53] DEBUG: Inner: 4242 covered: 5494 total: 6758 [2019-09-06 08:55:53] DEBUG: Assembled disjointig 49 With 4 reads Start read: +11ea8b0c-35d4-44d8-a7cc-9bf1b5cfa581 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 23 Avg overlap len: 5266 Min overlap len: 5238 Inner reads: 0 Length: 14454 [2019-09-06 08:55:53] DEBUG: Ovlp index size: 33541 [2019-09-06 08:55:53] DEBUG: Inner: 4246 covered: 5554 total: 6758 [2019-09-06 08:55:54] DEBUG: Assembled disjointig 50 With 4 reads Start read: +f2178b4e-ba30-4856-9bee-2af7d14cd7d9 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 21 Avg overlap len: 5298 Min overlap len: 5296 Inner reads: 0 Length: 35611 [2019-09-06 08:55:54] DEBUG: Ovlp index size: 33781 [2019-09-06 08:55:54] DEBUG: Inner: 4250 covered: 5586 total: 6758 [2019-09-06 08:55:54] DEBUG: Assembled disjointig 51 With 4 reads Start read: +32233226-7206-4b75-92ff-7c009360704c At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5413 Min overlap len: 5411 Inner reads: 0 Length: 16178 [2019-09-06 08:55:54] DEBUG: Ovlp index size: 33871 [2019-09-06 08:55:54] DEBUG: Inner: 4258 covered: 5618 total: 6758 [2019-09-06 08:55:55] DEBUG: Assembled disjointig 52 With 5 reads Start read: +c827bd58-7b5a-4527-8025-5aaaf916b81c At position: 4 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 9 Avg overlap len: 17111 Min overlap len: 7882 Inner reads: 0 Length: 53800 [2019-09-06 08:55:55] DEBUG: Ovlp index size: 34390 [2019-09-06 08:55:55] DEBUG: Inner: 4266 covered: 5742 total: 6758 [2019-09-06 08:55:56] DEBUG: Assembled disjointig 53 With 4 reads Start read: +996d0710-06a4-4d3b-bf5a-85bb98606dcd At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 24 Avg overlap len: 5130 Min overlap len: 5130 Inner reads: 0 Length: 31021 [2019-09-06 08:55:56] DEBUG: Ovlp index size: 35380 [2019-09-06 08:55:56] DEBUG: Inner: 4270 covered: 5988 total: 6758 [2019-09-06 08:55:57] DEBUG: Assembled disjointig 54 With 4 reads Start read: +963344d4-cfdd-4595-9c50-4f5cd8aeca88 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5493 Min overlap len: 5479 Inner reads: 0 Length: 35967 [2019-09-06 08:55:57] DEBUG: Ovlp index size: 35560 [2019-09-06 08:55:57] DEBUG: Inner: 4274 covered: 6012 total: 6758 [2019-09-06 08:55:57] DEBUG: Assembled disjointig 55 With 4 reads Start read: +39238b56-87f8-49b5-9648-3e7b4ef3e3ee At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 21 Avg overlap len: 5502 Min overlap len: 5491 Inner reads: 0 Length: 17781 [2019-09-06 08:55:57] DEBUG: Ovlp index size: 35710 [2019-09-06 08:55:57] DEBUG: Inner: 4280 covered: 6046 total: 6758 [2019-09-06 08:55:57] DEBUG: Assembled disjointig 56 With 5 reads Start read: +9f5ee665-9b96-457f-9ac5-3978135607d1 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 19 Avg overlap len: 9902 Min overlap len: 5019 Inner reads: 0 Length: 17919 [2019-09-06 08:55:57] DEBUG: Ovlp index size: 36081 [2019-09-06 08:55:57] DEBUG: Inner: 4290 covered: 6118 total: 6758 [2019-09-06 08:55:58] DEBUG: Assembled disjointig 57 With 4 reads Start read: +c3ed8661-cfc5-45bd-94af-da83de209603 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5494 Min overlap len: 5489 Inner reads: 0 Length: 17713 [2019-09-06 08:55:58] DEBUG: Ovlp index size: 36793 [2019-09-06 08:55:58] DEBUG: Inner: 4294 covered: 6260 total: 6758 [2019-09-06 08:55:58] DEBUG: Assembled disjointig 58 With 4 reads Start read: +b2699a05-1e2f-48d5-b8c1-481a95e7129d At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 18 Avg overlap len: 6136 Min overlap len: 6127 Inner reads: 0 Length: 17159 [2019-09-06 08:55:58] DEBUG: Ovlp index size: 36883 [2019-09-06 08:55:58] DEBUG: Inner: 4298 covered: 6268 total: 6758 [2019-09-06 08:55:58] DEBUG: Assembled disjointig 59 With 4 reads Start read: +88c59ccd-23d0-4890-b4b1-ea5ab952e96f At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 13 Avg overlap len: 22150 Min overlap len: 21850 Inner reads: 0 Length: 73508 [2019-09-06 08:55:58] DEBUG: Ovlp index size: 36943 [2019-09-06 08:55:58] DEBUG: Inner: 4316 covered: 6294 total: 6758 [2019-09-06 08:55:58] DEBUG: Assembled disjointig 60 With 4 reads Start read: +edcbde44-cd96-4113-9769-4b99b7f6b8d3 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5985 Min overlap len: 5961 Inner reads: 0 Length: 21382 [2019-09-06 08:55:58] DEBUG: Ovlp index size: 37033 [2019-09-06 08:55:58] DEBUG: Inner: 4320 covered: 6314 total: 6758 [2019-09-06 08:55:58] DEBUG: Assembled disjointig 61 With 4 reads Start read: +6895cc1c-6e52-4690-a868-19c3b8127473 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5059 Min overlap len: 5058 Inner reads: 0 Length: 23912 [2019-09-06 08:55:58] DEBUG: Ovlp index size: 37033 [2019-09-06 08:55:58] DEBUG: Inner: 4324 covered: 6316 total: 6758 [2019-09-06 08:55:59] DEBUG: Assembled disjointig 62 With 4 reads Start read: +023b7e2a-6e40-4950-92f4-060f0fa2bc60 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5062 Min overlap len: 5062 Inner reads: 0 Length: 18417 [2019-09-06 08:55:59] DEBUG: Ovlp index size: 37123 [2019-09-06 08:55:59] DEBUG: Inner: 4332 covered: 6344 total: 6758 [2019-09-06 08:55:59] DEBUG: Assembled disjointig 63 With 4 reads Start read: +1324c6f6-5fe4-4958-a7fa-fa85b13174a8 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 8 Avg overlap len: 8141 Min overlap len: 7769 Inner reads: 0 Length: 130144 [2019-09-06 08:55:59] DEBUG: Ovlp index size: 37469 [2019-09-06 08:55:59] DEBUG: Inner: 4340 covered: 6468 total: 6758 [2019-09-06 08:56:00] DEBUG: Assembled disjointig 64 With 4 reads Start read: +2d1d605d-3d24-41db-a6ff-897eab95cbac At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 16 Avg overlap len: 5636 Min overlap len: 5627 Inner reads: 0 Length: 16369 [2019-09-06 08:56:00] DEBUG: Ovlp index size: 37559 [2019-09-06 08:56:00] DEBUG: Inner: 4346 covered: 6504 total: 6758 [2019-09-06 08:56:00] DEBUG: Assembled disjointig 65 With 4 reads Start read: +7379c841-dae1-4156-9ad0-25872fba49ab At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 24 Avg overlap len: 5487 Min overlap len: 5481 Inner reads: 0 Length: 26062 [2019-09-06 08:56:00] DEBUG: Ovlp index size: 37649 [2019-09-06 08:56:00] DEBUG: Inner: 4350 covered: 6514 total: 6758 [2019-09-06 08:56:00] DEBUG: Assembled disjointig 66 With 4 reads Start read: +e8018bf4-0f88-4e43-8131-82347bdca947 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 20 Avg overlap len: 5798 Min overlap len: 5796 Inner reads: 0 Length: 23298 [2019-09-06 08:56:00] DEBUG: Ovlp index size: 37739 [2019-09-06 08:56:00] DEBUG: Inner: 4354 covered: 6528 total: 6758 [2019-09-06 08:56:00] DEBUG: Assembled disjointig 67 With 4 reads Start read: +4eb88aba-5a0a-48de-812f-8fde6dc62ab1 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 25 Avg overlap len: 5248 Min overlap len: 5247 Inner reads: 0 Length: 24039 [2019-09-06 08:56:00] DEBUG: Ovlp index size: 38069 [2019-09-06 08:56:00] DEBUG: Inner: 4358 covered: 6588 total: 6758 [2019-09-06 08:56:01] DEBUG: Assembled disjointig 68 With 4 reads Start read: +94d85514-1b03-43ba-80f4-a6020d8a3495 At position: 2 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 22 Avg overlap len: 5117 Min overlap len: 5117 Inner reads: 0 Length: 19237 [2019-09-06 08:56:01] DEBUG: Ovlp index size: 38129 [2019-09-06 08:56:01] DEBUG: Inner: 4364 covered: 6638 total: 6758 [2019-09-06 08:56:01] DEBUG: Assembled disjointig 69 With 4 reads Start read: +24982664-ea1e-4052-94d7-133a31fce441 At position: 3 leftTip: 0 rightTip: 0 Suspicious: 0 Mean extensions: 8 Avg overlap len: 11283 Min overlap len: 7793 Inner reads: 0 Length: 54149 [2019-09-06 08:56:01] DEBUG: Ovlp index size: 38262 [2019-09-06 08:56:01] DEBUG: Inner: 4374 covered: 6660 total: 6758 [2019-09-06 08:56:02] INFO: Assembled 69 disjointigs [2019-09-06 08:56:02] INFO: Generating sequence [2019-09-06 08:56:47] DEBUG: Writing FASTA [2019-09-06 08:56:47] DEBUG: Peak RAM usage: 2 Gb -----------End assembly log------------ [2019-09-06 08:56:47] root: DEBUG: Disjointigs length: 7218394, N50: 1010706 [2019-09-06 08:56:47] root: INFO: >>>STAGE: consensus [2019-09-06 08:56:47] root: INFO: Running Minimap2 [2019-09-06 08:57:01] root: INFO: Computing consensus [2019-09-06 08:57:41] root: INFO: Alignment error rate: 0.163103812516 [2019-09-06 08:57:41] root: INFO: >>>STAGE: repeat [2019-09-06 08:57:41] root: INFO: Building and resolving repeat graph [2019-09-06 08:57:41] root: DEBUG: -----Begin repeat analyser log------ [2019-09-06 08:57:41] root: DEBUG: Running: flye-repeat -l /data/user2/work/84/output/flye.log -t 8 -v 5000 -k 15 /data/user2/work/84/output/10-consensus/consensus.fasta all.fastq /data/user2/work/84/output/20-repeat /root/miniconda2/lib/python2.7/site-packages/flye/config/bin_cfg/asm_raw_reads.cfg [2019-09-06 08:57:41] DEBUG: Build date: Apr 7 2019 02:34:11 [2019-09-06 08:57:41] DEBUG: Total RAM: 204 Gb [2019-09-06 08:57:41] DEBUG: Available RAM: 199 Gb [2019-09-06 08:57:41] DEBUG: Total CPUs: 32 [2019-09-06 08:57:41] DEBUG: Parameters: [2019-09-06 08:57:41] DEBUG: big_genome_threshold=29000000 [2019-09-06 08:57:41] DEBUG: low_cutoff_warning=1 [2019-09-06 08:57:41] DEBUG: hard_min_coverage_rate=10 [2019-09-06 08:57:41] DEBUG: assemble_kmer_sample=1 [2019-09-06 08:57:41] DEBUG: repeat_graph_kmer_sample=1 [2019-09-06 08:57:41] DEBUG: read_align_kmer_sample=1 [2019-09-06 08:57:41] DEBUG: maximum_jump=1500 [2019-09-06 08:57:41] DEBUG: maximum_overhang=1500 [2019-09-06 08:57:41] DEBUG: repeat_kmer_rate=100 [2019-09-06 08:57:41] DEBUG: assemble_ovlp_divergence=0.30 [2019-09-06 08:57:41] DEBUG: repeat_graph_ovlp_divergence=0.15 [2019-09-06 08:57:41] DEBUG: repeat_graph_ovlp_end_adjust=0.00 [2019-09-06 08:57:41] DEBUG: read_align_ovlp_divergence=0.25 [2019-09-06 08:57:41] DEBUG: max_coverage_drop_rate=5 [2019-09-06 08:57:41] DEBUG: chimera_window=100 [2019-09-06 08:57:41] DEBUG: min_reads_in_disjointig=4 [2019-09-06 08:57:41] DEBUG: max_inner_reads=10 [2019-09-06 08:57:41] DEBUG: max_inner_fraction=0.25 [2019-09-06 08:57:41] DEBUG: add_unassembled_reads=0 [2019-09-06 08:57:41] DEBUG: max_separation=500 [2019-09-06 08:57:41] DEBUG: tip_length_threshold=100000 [2019-09-06 08:57:41] DEBUG: unique_edge_length=50000 [2019-09-06 08:57:41] DEBUG: min_repeat_res_support=0.51 [2019-09-06 08:57:41] DEBUG: out_paths_ratio=5 [2019-09-06 08:57:41] DEBUG: graph_cov_drop_rate=10 [2019-09-06 08:57:41] DEBUG: coverage_estimate_window=100 [2019-09-06 08:57:41] DEBUG: extend_contigs_with_repeats=1 [2019-09-06 08:57:41] DEBUG: Running with k-mer size: 15 [2019-09-06 08:57:41] DEBUG: Selected minimum overlap 5000 [2019-09-06 08:57:41] DEBUG: Metagenome mode: N [2019-09-06 08:57:41] INFO: Reading sequences [2019-09-06 08:57:41] DEBUG: Building positional index [2019-09-06 08:57:41] DEBUG: Total sequence: 65381103 bp [2019-09-06 08:57:41] DEBUG: Building positional index [2019-09-06 08:57:41] DEBUG: Total sequence: 6711853 bp [2019-09-06 08:57:41] INFO: Building repeat graph [2019-09-06 08:57:41] DEBUG: Hard threshold set to 1 [2019-09-06 08:57:41] DEBUG: Started k-mer counting [2019-09-06 08:58:06] DEBUG: Repetitive k-mer frequency: 122 [2019-09-06 08:58:06] DEBUG: Filtered 0 repetitive k-mers (0) [2019-09-06 08:58:06] DEBUG: Sampling rate: 1 [2019-09-06 08:58:06] DEBUG: Solid k-mers: 678180 [2019-09-06 08:58:06] DEBUG: K-mer index size: 1927184 [2019-09-06 08:58:06] DEBUG: Mean k-mer frequency: 2.8417 [2019-09-06 08:58:07] DEBUG: Sorting k-mer index [2019-09-06 08:58:28] DEBUG: Computing transitive closure for overlaps [2019-09-06 08:58:28] DEBUG: Found 4284 overlaps [2019-09-06 08:58:28] DEBUG: Left 1740 overlaps after filtering [2019-09-06 08:58:28] INFO: Median overlap divergence: 0.138254 [2019-09-06 08:58:28] DEBUG: Sequence divergence distribution: | * * | * * | * * | * * * | * * * | * * * | * * * * * | * * * * * | * *** * * * | * *** * * * | * ** *** * * * | * * ** *** ** * * * | * * ** *** ** * * * | * * ** ******** * ** | * * ** ******** * ** | * * * *************** ** * * | ** *** *** *************** ** ** * * * | ** *** *** *************** ** ** * * * | ********** ****************** ** ** * ** | ********** ****************** ** ** * ** ---------------------------------------------------------------------------------------------------- 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% Q25 = 0.11, Q50 = 0.14, Q75 = 0.17 [2019-09-06 08:58:28] DEBUG: Computing gluepoints [2019-09-06 08:58:28] DEBUG: Added 154 gluepoint projections [2019-09-06 08:58:28] DEBUG: Merging 19 -> 19 [2019-09-06 08:58:28] DEBUG: Merging 18 -> 19 [2019-09-06 08:58:28] DEBUG: Merging 21 -> 22 [2019-09-06 08:58:28] DEBUG: Merging 77 -> 76 [2019-09-06 08:58:28] DEBUG: Merging 74 -> 73 [2019-09-06 08:58:28] DEBUG: Merging 22 -> 22 [2019-09-06 08:58:28] DEBUG: Merging 73 -> 73 [2019-09-06 08:58:28] DEBUG: Merging 76 -> 76 [2019-09-06 08:58:28] DEBUG: Added 0 gluepoint projections [2019-09-06 08:58:28] DEBUG: Created 2290 gluepoints [2019-09-06 08:58:28] DEBUG: Tandems removed: 58 left, 58 right, 8 both [2019-09-06 08:58:28] DEBUG: Initializing edges [2019-09-06 08:58:28] DEBUG: Filtered 2 singleton segments [2019-09-06 08:58:28] DEBUG: Removed 0 simple and 0 double chimeric junctions [2019-09-06 08:58:28] DEBUG: Collapsed 42 edges [2019-09-06 08:58:28] DEBUG: Collapsed 1 bulges [2019-09-06 08:58:28] DEBUG: Collapsed 1 edges [2019-09-06 08:58:28] DEBUG: * 51 +disjointig_12 0 17473 17473 [2019-09-06 08:58:28] DEBUG: * -28 +disjointig_34 0 125 125 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_17 4873 5729 856 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_50 4575 5372 797 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_11 20 557 537 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_11 557 2470 1913 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_11 2470 3074 604 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_11 3074 3896 822 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_11 3896 5716 1820 (3) [2019-09-06 08:58:28] DEBUG: -37 +disjointig_23 394 1100 706 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_23 1100 3023 1923 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_23 3023 3607 584 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_23 3607 4378 771 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_23 4378 5771 1393 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_23 5771 6484 713 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_23 6484 7425 941 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_23 7425 8305 880 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_58 1 887 886 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_58 887 3446 2559 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_58 3446 4319 873 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_58 4319 9809 5490 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_58 9809 10668 859 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_64 123 2320 2197 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_64 2320 2957 637 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_64 2957 3594 637 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_64 3594 8991 5397 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_64 8991 9856 865 [2019-09-06 08:58:28] DEBUG: * -50 +disjointig_24 0 5245 5245 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_38 13 942 929 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_38 942 1715 773 [2019-09-06 08:58:28] DEBUG: -83 +disjointig_38 1715 13860 12145 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_8 8 1764 1756 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_8 1764 2822 1058 (2) [2019-09-06 08:58:28] DEBUG: -40 +disjointig_8 2822 3634 812 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_8 3634 4869 1235 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_8 4869 5542 673 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_8 5542 6312 770 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_8 6312 7527 1215 (2) [2019-09-06 08:58:28] DEBUG: -86 +disjointig_8 7527 9617 2090 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_8 9617 10252 635 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_8 10252 10888 636 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_8 10888 16334 5446 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_8 16334 17251 917 [2019-09-06 08:58:28] DEBUG: 81 +disjointig_8 17251 26160 8909 [2019-09-06 08:58:28] DEBUG: * -77 +disjointig_4 110 268128 268018 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_4 268128 273213 5085 [2019-09-06 08:58:28] DEBUG: * -84 +disjointig_4 273213 357690 84477 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_4 357690 362947 5257 [2019-09-06 08:58:28] DEBUG: * 75 +disjointig_4 362947 483343 120396 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_4 483343 488707 5364 [2019-09-06 08:58:28] DEBUG: * 74 +disjointig_4 488707 523127 34420 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_4 523127 528155 5028 [2019-09-06 08:58:28] DEBUG: * -90 +disjointig_4 528155 939549 411394 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_54 7925 8712 787 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_54 8712 10081 1369 (2) [2019-09-06 08:58:28] DEBUG: -85 +disjointig_54 10081 12466 2385 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_54 12466 13024 558 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_54 13024 14663 1639 (2) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_54 14663 15472 809 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_54 15472 16230 758 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_54 16230 17625 1395 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_54 17625 18382 757 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_54 18382 19387 1005 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_54 19387 20314 927 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_54 20314 22891 2577 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_54 22891 23725 834 [2019-09-06 08:58:28] DEBUG: 83 +disjointig_42 229 12274 12045 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_42 12274 13037 763 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_42 13037 14404 1367 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_42 14404 16789 2385 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_42 16789 17303 514 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_42 17303 19061 1758 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_42 19061 19869 808 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_42 19869 20695 826 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_42 20695 22126 1431 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_42 22126 22875 749 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_42 22875 23886 1011 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_42 23886 24801 915 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_42 24801 26936 2135 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_42 26936 27722 786 [2019-09-06 08:58:28] DEBUG: 34 +disjointig_7 6006 6556 550 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_7 6556 11834 5278 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_7 11834 12451 617 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_7 12451 13069 618 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_7 13069 15181 2112 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_7 15181 16196 1015 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_7 16196 17181 985 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_7 17181 17724 543 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_7 17724 19355 1631 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_7 19355 20179 824 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_7 20179 20793 614 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_7 20793 22757 1964 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_7 22757 23432 675 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_7 23432 25664 2232 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_7 25664 27034 1370 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_7 27034 27871 837 [2019-09-06 08:58:28] DEBUG: -81 +disjointig_1 2 8963 8961 [2019-09-06 08:58:28] DEBUG: 34 +disjointig_1 8963 9872 909 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_1 9872 15315 5443 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_1 15315 15945 630 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_1 15945 16575 630 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_1 16575 18680 2105 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_1 18680 19916 1236 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_1 19916 20682 766 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_1 20682 21228 546 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_1 21228 22866 1638 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_1 22866 23662 796 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_1 23662 24319 657 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_1 24319 26202 1883 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_1 26202 26928 726 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_1 26928 29055 2127 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_1 29055 30513 1458 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_1 30513 31426 913 [2019-09-06 08:58:28] DEBUG: -83 +disjointig_1 31426 44216 12790 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_40 10069 11002 933 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_40 11002 12428 1426 (2) [2019-09-06 08:58:28] DEBUG: -85 +disjointig_40 12428 14540 2112 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_40 14540 15266 726 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_40 15266 17159 1893 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_40 17159 17818 659 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_40 17818 18615 797 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_40 18615 19910 1295 (2) [2019-09-06 08:58:28] DEBUG: 34 +disjointig_2 1047 1890 843 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_2 1890 7281 5391 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_2 7281 7918 637 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_2 7918 8555 637 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_2 8555 10676 2121 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_2 10676 11677 1001 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_2 11677 12674 997 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_2 12674 13400 726 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_2 13400 14595 1195 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_2 14595 15388 793 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_2 15388 15909 521 [2019-09-06 08:58:28] DEBUG: 34 +disjointig_45 5993 6848 855 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_45 6848 12319 5471 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_45 12319 12951 632 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_45 12951 13584 633 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_45 13584 15654 2070 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_45 15654 16608 954 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_45 16608 17588 980 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_45 17588 18127 539 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_45 18127 19746 1619 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_45 19746 20527 781 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_45 20527 21068 541 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_45 21068 23232 2164 (4) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_45 23232 23774 542 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_30 1 1097 1096 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_30 1097 3309 2212 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_30 3309 4217 908 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_30 4217 5203 986 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_30 5203 6036 833 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_30 6036 7109 1073 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_30 7109 8410 1301 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_30 8410 8980 570 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_30 8980 10620 1640 (2) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_30 10620 11151 531 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_30 11151 13156 2005 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_30 13156 14264 1108 (2) [2019-09-06 08:58:28] DEBUG: 34 +disjointig_41 4193 5022 829 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_41 5022 10475 5453 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_41 10475 11101 626 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_41 11101 11728 627 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_41 11728 13799 2071 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_41 13799 14803 1004 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_41 14803 15818 1015 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_41 15818 16537 719 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_41 16537 17677 1140 (2) [2019-09-06 08:58:28] DEBUG: 35 +disjointig_13 1730 2347 617 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_13 2347 2965 618 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_13 2965 5043 2078 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_13 5043 6042 999 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_13 6042 7020 978 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_13 7020 7558 538 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_13 7558 9174 1616 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_13 9174 9980 806 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_13 9980 10647 667 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_13 10647 12521 1874 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_13 12521 13215 694 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_65 2328 2966 638 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_65 2966 3604 638 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_65 3604 5690 2086 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_65 5690 6905 1215 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_65 6905 7675 770 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_65 7675 8219 544 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_65 8219 9852 1633 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_65 9852 10410 558 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_65 10410 11462 1052 (2) [2019-09-06 08:58:28] DEBUG: 41 +disjointig_65 11462 13222 1760 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_65 13222 13944 722 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_65 13944 15915 1971 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_65 15915 17161 1246 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_65 17161 18756 1595 (3) [2019-09-06 08:58:28] DEBUG: -44 +disjointig_31 4185 5529 1344 (2) [2019-09-06 08:58:28] DEBUG: -43 +disjointig_31 5529 6518 989 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_31 6518 8919 2401 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_31 8919 9442 523 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_31 9442 11216 1774 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_31 11216 12018 802 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_31 12018 13159 1141 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_56 1726 2348 622 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_56 2348 2971 623 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_56 2971 5058 2087 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_56 5058 6031 973 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_56 6031 7045 1014 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_56 7045 7793 748 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_56 7793 9213 1420 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_56 9213 10020 807 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_56 10020 10599 579 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_56 10599 12441 1842 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_56 12441 13059 618 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_56 13059 15389 2330 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_56 15389 16330 941 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_56 16330 17020 690 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_36 1 641 640 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_36 641 1388 747 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_36 1388 2155 767 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_36 2155 3564 1409 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_36 3564 4339 775 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_36 4339 4923 584 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_36 4923 6779 1856 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_36 6779 7287 508 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_36 7287 9748 2461 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_36 9748 11021 1273 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_36 11021 11792 771 [2019-09-06 08:58:28] DEBUG: 34 +disjointig_53 5916 6775 859 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_53 6775 12232 5457 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_53 12232 12861 629 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_53 12861 13491 630 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_53 13491 15477 1986 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_53 15477 16457 980 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_53 16457 17456 999 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_53 17456 18185 729 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_53 18185 19609 1424 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_53 19609 20412 803 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_53 20412 21070 658 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_53 21070 22820 1750 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_53 22820 23332 512 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_53 23332 25661 2329 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_53 25661 26978 1317 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_53 26978 27746 768 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_57 15 5524 5509 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_57 5524 6167 643 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_57 6167 6810 643 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_57 6810 8980 2170 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_57 8980 9856 876 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_57 9856 10822 966 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_57 10822 11369 547 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_57 11369 13010 1641 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_57 13010 13808 798 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_57 13808 14405 597 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_57 14405 16252 1847 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_57 16252 16824 572 [2019-09-06 08:58:28] DEBUG: * 78 +disjointig_3 83359 306545 223186 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_3 306545 311883 5338 [2019-09-06 08:58:28] DEBUG: * -87 +disjointig_3 311883 973637 661754 [2019-09-06 08:58:28] DEBUG: 76 +disjointig_3 973637 978716 5079 [2019-09-06 08:58:28] DEBUG: * 80 +disjointig_3 978716 3311786 2333070 [2019-09-06 08:58:28] DEBUG: -76 +disjointig_3 3311786 3316811 5025 [2019-09-06 08:58:28] DEBUG: * 89 +disjointig_3 3316811 3516726 199915 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_60 4707 5448 741 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_60 5448 7416 1968 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_60 7416 8372 956 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_60 8372 9317 945 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_60 9317 10171 854 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_60 10171 11219 1048 [2019-09-06 08:58:28] DEBUG: 40 +disjointig_60 11219 12287 1068 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_60 12287 13023 736 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_60 13023 14855 1832 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_60 14855 15366 511 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_60 15366 17871 2505 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_60 17871 18885 1014 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_60 18885 19655 770 [2019-09-06 08:58:28] DEBUG: -76 +disjointig_26 40504 45571 5067 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_61 76 625 549 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_61 625 1600 975 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_61 1600 2141 541 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_61 2141 3766 1625 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_61 3766 4564 798 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_61 4564 5233 669 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_61 5233 7066 1833 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_61 7066 7841 775 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_61 7841 9983 2142 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_61 9983 11417 1434 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_61 11417 12216 799 [2019-09-06 08:58:28] DEBUG: 34 +disjointig_5 6373 7276 903 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_5 7276 12738 5462 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_5 12738 13379 641 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_5 13379 14020 641 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_5 14020 16086 2066 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_5 16086 17298 1212 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_5 17298 18053 755 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_5 18053 18597 544 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_5 18597 20229 1632 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_5 20229 21035 806 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_5 21035 21681 646 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_5 21681 23549 1868 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_5 23549 24266 717 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_5 24266 26220 1954 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_5 26220 27806 1586 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_5 27806 28777 971 [2019-09-06 08:58:28] DEBUG: -83 +disjointig_5 28777 40773 11996 [2019-09-06 08:58:28] DEBUG: 83 +disjointig_55 392 12935 12543 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_55 12935 13725 790 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_55 13725 15097 1372 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_55 15097 17009 1912 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_55 17009 17560 551 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_49 0 626 626 [2019-09-06 08:58:28] DEBUG: -42 +disjointig_49 626 1348 722 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_49 1348 2278 930 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_49 2278 3264 986 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_49 3264 5337 2073 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_49 5337 5968 631 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_49 5968 6600 632 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_49 6600 12388 5788 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_22 0 534 534 [2019-09-06 08:58:28] DEBUG: -39 +disjointig_22 534 1598 1064 (2) [2019-09-06 08:58:28] DEBUG: -40 +disjointig_22 1598 2428 830 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_22 2428 3686 1258 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_22 3686 4373 687 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_22 4373 5343 970 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_22 5343 6324 981 [2019-09-06 08:58:28] DEBUG: -86 +disjointig_22 6324 8421 2097 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_22 8421 9051 630 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_22 9051 9682 631 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_22 9682 15194 5512 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_22 15194 16039 845 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_18 1317 1938 621 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_18 1938 2560 622 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_18 2560 4601 2041 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_18 4601 5596 995 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_18 5596 6591 995 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_18 6591 7142 551 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_18 7142 8795 1653 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_18 8795 9596 801 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_18 9596 10262 666 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_18 10262 12161 1899 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_18 12161 12882 721 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_18 12882 15027 2145 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_18 15027 15951 924 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_18 15951 17231 1280 (2) [2019-09-06 08:58:28] DEBUG: 86 +disjointig_32 97 2695 2598 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_32 2695 3593 898 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_32 3593 4595 1002 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_32 4595 5283 688 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_32 5283 6684 1401 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_32 6684 7481 797 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_32 7481 8142 661 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_32 8142 9978 1836 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_32 9978 10512 534 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_32 10512 12936 2424 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_16 4252 4851 599 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_16 4851 5451 600 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_16 5451 7584 2133 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_16 7584 8502 918 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_16 8502 9490 988 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_16 9490 10243 753 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_16 10243 11684 1441 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_16 11684 12482 798 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_16 12482 13290 808 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_16 13290 15508 2218 (3) [2019-09-06 08:58:28] DEBUG: 85 +disjointig_16 15508 17876 2368 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_16 17876 18818 942 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_16 18818 20123 1305 (2) [2019-09-06 08:58:28] DEBUG: 34 +disjointig_6 5936 6805 869 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_6 6805 8987 2182 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_6 8987 9902 915 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_6 9902 10788 886 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_6 10788 11675 887 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_6 11675 12758 1083 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_6 12758 13587 829 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_6 13587 14648 1061 (2) [2019-09-06 08:58:28] DEBUG: 41 +disjointig_6 14648 16599 1951 (3) [2019-09-06 08:58:28] DEBUG: 34 +disjointig_66 654 1552 898 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_66 1552 6967 5415 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_66 6967 7599 632 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_66 7599 8232 633 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_66 8232 10322 2090 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_66 10322 11573 1251 (2) [2019-09-06 08:58:28] DEBUG: 27 +disjointig_66 11573 12306 733 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_66 12306 12848 542 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_66 12848 14477 1629 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_66 14477 15275 798 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_66 15275 15933 658 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_66 15933 17819 1886 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_66 17819 18537 718 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_66 18537 20673 2136 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_66 20673 22095 1422 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_66 22095 22880 785 [2019-09-06 08:58:28] DEBUG: * -67 +disjointig_48 0 38090 38090 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_67 2034 2662 628 [2019-09-06 08:58:28] DEBUG: 33 +disjointig_67 2662 3291 629 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_67 3291 5362 2071 [2019-09-06 08:58:28] DEBUG: 36 +disjointig_67 5362 6357 995 [2019-09-06 08:58:28] DEBUG: 27 +disjointig_67 6357 7370 1013 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_67 7370 8078 708 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_67 8078 9489 1411 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_67 9489 10280 791 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_67 10280 10921 641 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_67 10921 12775 1854 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_67 12775 13477 702 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_67 13477 15659 2182 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_67 15659 16697 1038 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_67 16697 17998 1301 (2) [2019-09-06 08:58:28] DEBUG: -43 +disjointig_35 10 526 516 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_35 526 2947 2421 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_35 2947 3497 550 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_35 3497 5276 1779 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_35 5276 5834 558 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_35 5834 6862 1028 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_35 6862 8200 1338 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_35 8200 9049 849 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_35 9049 10058 1009 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_35 10058 11060 1002 (2) [2019-09-06 08:58:28] DEBUG: -86 +disjointig_35 11060 13546 2486 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_35 13546 14390 844 [2019-09-06 08:58:28] DEBUG: 82 +disjointig_35 14390 19749 5359 [2019-09-06 08:58:28] DEBUG: -34 +disjointig_35 19749 20606 857 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_27 5 2224 2219 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_27 2224 2944 720 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_27 2944 4710 1766 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_27 4710 5774 1064 (2) [2019-09-06 08:58:28] DEBUG: -40 +disjointig_27 5774 6336 562 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_27 6336 7980 1644 (3) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_27 7980 8529 549 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_27 8529 9268 739 [2019-09-06 08:58:28] DEBUG: -36 +disjointig_27 9268 10492 1224 (2) [2019-09-06 08:58:28] DEBUG: -86 +disjointig_27 10492 12603 2111 [2019-09-06 08:58:28] DEBUG: -33 +disjointig_27 12603 13244 641 [2019-09-06 08:58:28] DEBUG: -35 +disjointig_27 13244 13885 641 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_25 5807 6616 809 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_25 6616 7945 1329 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_25 7945 10179 2234 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_25 10179 10861 682 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_25 10861 12553 1692 (2) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_29 1 769 768 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_29 769 1350 581 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_29 1350 3676 2326 (3) [2019-09-06 08:58:28] DEBUG: 85 +disjointig_29 3676 5922 2246 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_29 5922 7374 1452 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_29 7374 8120 746 [2019-09-06 08:58:28] DEBUG: -83 +disjointig_29 8120 21091 12971 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_19 4 1627 1623 (2) [2019-09-06 08:58:28] DEBUG: -43 +disjointig_19 1627 2621 994 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_19 2621 5001 2380 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_19 5001 5514 513 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_19 5514 7290 1776 (3) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_19 7290 8106 816 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_19 8106 9164 1058 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_15 22 1849 1827 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_15 1849 2377 528 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_15 2377 4605 2228 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_15 4605 5872 1267 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_15 5872 7437 1565 (2) [2019-09-06 08:58:28] DEBUG: -83 +disjointig_15 7437 19959 12522 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_47 8771 9585 814 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_47 9585 10938 1353 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_47 10938 13164 2226 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_47 13164 13823 659 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_47 13823 15496 1673 (2) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_47 15496 16149 653 [2019-09-06 08:58:28] DEBUG: 42 +disjointig_62 20 559 539 [2019-09-06 08:58:28] DEBUG: 38 +disjointig_62 559 2179 1620 (3) [2019-09-06 08:58:28] DEBUG: 40 +disjointig_62 2179 2987 808 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_62 2987 3581 594 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_62 3581 5430 1849 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_62 5430 6194 764 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_62 6194 8393 2199 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_62 8393 9715 1322 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_62 9715 10514 799 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_68 1 733 732 [2019-09-06 08:58:28] DEBUG: 37 +disjointig_68 733 1254 521 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_68 1254 3430 2176 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_68 3430 4653 1223 (2) [2019-09-06 08:58:28] DEBUG: 44 +disjointig_68 4653 5753 1100 (2) [2019-09-06 08:58:28] DEBUG: 34 +disjointig_51 5968 6488 520 [2019-09-06 08:58:28] DEBUG: -82 +disjointig_51 6488 12300 5812 [2019-09-06 08:58:28] DEBUG: 35 +disjointig_51 12300 13190 890 [2019-09-06 08:58:28] DEBUG: 86 +disjointig_51 13190 15720 2530 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_20 1 1038 1037 (2) [2019-09-06 08:58:28] DEBUG: 41 +disjointig_20 1038 1540 502 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_20 3171 5213 2042 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_20 5213 5818 605 [2019-09-06 08:58:28] DEBUG: -44 +disjointig_21 8731 9466 735 [2019-09-06 08:58:28] DEBUG: -43 +disjointig_21 9466 10768 1302 [2019-09-06 08:58:28] DEBUG: -85 +disjointig_21 10768 13201 2433 [2019-09-06 08:58:28] DEBUG: -37 +disjointig_21 13201 13721 520 [2019-09-06 08:58:28] DEBUG: -41 +disjointig_21 13721 15255 1534 (2) [2019-09-06 08:58:28] DEBUG: -39 +disjointig_21 15255 16034 779 [2019-09-06 08:58:28] DEBUG: -40 +disjointig_21 16034 16812 778 [2019-09-06 08:58:28] DEBUG: -38 +disjointig_21 16812 18123 1311 (2) [2019-09-06 08:58:28] DEBUG: -42 +disjointig_21 18123 19005 882 [2019-09-06 08:58:28] DEBUG: -27 +disjointig_21 19005 19774 769 [2019-09-06 08:58:28] DEBUG: 39 +disjointig_10 58 791 733 [2019-09-06 08:58:28] DEBUG: 41 +disjointig_10 791 2576 1785 (3) [2019-09-06 08:58:28] DEBUG: 37 +disjointig_10 2576 3288 712 [2019-09-06 08:58:28] DEBUG: 85 +disjointig_10 3288 5513 2225 [2019-09-06 08:58:28] DEBUG: 43 +disjointig_10 5513 6478 965 [2019-09-06 08:58:28] DEBUG: 44 +disjointig_10 6478 7762 1284 (2) [2019-09-06 08:58:28] DEBUG: Total edges: 90 [2019-09-06 08:58:28] DEBUG: Building positional index [2019-09-06 08:58:28] DEBUG: Total sequence: 5144957 bp [2019-09-06 08:58:28] INFO: Aligning reads to the graph [2019-09-06 08:58:28] DEBUG: Hard threshold set to 1 [2019-09-06 08:58:28] DEBUG: Started k-mer counting [2019-09-06 08:58:50] DEBUG: Repetitive k-mer frequency: 111 [2019-09-06 08:58:50] DEBUG: Filtered 0 repetitive k-mers (0) [2019-09-06 08:58:50] DEBUG: Sampling rate: 1 [2019-09-06 08:58:50] DEBUG: Solid k-mers: 4590979 [2019-09-06 08:58:50] DEBUG: K-mer index size: 5135222 [2019-09-06 08:58:50] DEBUG: Mean k-mer frequency: 1.11855 [2019-09-06 08:58:52] DEBUG: Sorting k-mer index [2019-09-06 08:59:00] DEBUG: Total reads : 3379 [2019-09-06 08:59:00] DEBUG: Read with aligned parts : 3308 [2019-09-06 08:59:00] DEBUG: Aligned in one piece : 3275 [2019-09-06 08:59:00] INFO: Aligned read sequence: 64258546 / 65381103 (0.982831) [2019-09-06 08:59:00] INFO: Median overlap divergence: 0.0893004 [2019-09-06 08:59:00] DEBUG: Sequence divergence distribution: | * | * | ** | ** | *** | **** | **** | ***** | ***** | ****** | ****** | ******* | ******* | ******** ** | ******** **** | ********* ***** | ********** ******** | ********************** | ************************ |** ************************************************************************************ ---------------------------------------------------------------------------------------------------- 0% 5% 10% 15% 20% 25% 30% 35% 40% 45% Q25 = 0.074, Q50 = 0.089, Q75 = 0.14 [2019-09-06 08:59:00] INFO: Mean edge coverage: 13 [2019-09-06 08:59:00] DEBUG: 90 len:411394 cov:13 mult:1 [2019-09-06 08:59:00] DEBUG: -89 len:199915 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: 89 len:199915 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: 86 len:2175 cov:291 mult:22.3846 [2019-09-06 08:59:00] DEBUG: -85 len:2235 cov:248 mult:19.0769 [2019-09-06 08:59:00] DEBUG: 85 len:2235 cov:248 mult:19.0769 [2019-09-06 08:59:00] DEBUG: -84 len:84477 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: 84 len:84477 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: 83 len:12430 cov:212 mult:16.3077 [2019-09-06 08:59:00] DEBUG: -81 len:8935 cov:187 mult:14.3846 [2019-09-06 08:59:00] DEBUG: 81 len:8935 cov:187 mult:14.3846 [2019-09-06 08:59:00] DEBUG: -80 len:2333070 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: 80 len:2333070 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: -77 len:268018 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: -74 len:34420 cov:8 mult:0.615385 [2019-09-06 08:59:00] DEBUG: -67 len:38090 cov:2 mult:0.153846 [2019-09-06 08:59:00] DEBUG: 67 len:38090 cov:2 mult:0.153846 [2019-09-06 08:59:00] DEBUG: -83 len:12430 cov:212 mult:16.3077 [2019-09-06 08:59:00] DEBUG: 76 len:5155 cov:62 mult:4.76923 [2019-09-06 08:59:00] DEBUG: -76 len:5155 cov:62 mult:4.76923 [2019-09-06 08:59:00] DEBUG: -51 len:17473 cov:1 mult:0.0769231 [2019-09-06 08:59:00] DEBUG: -50 len:5245 cov:1 mult:0.0769231 [2019-09-06 08:59:00] DEBUG: 50 len:5245 cov:1 mult:0.0769231 [2019-09-06 08:59:00] DEBUG: -87 len:661754 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: -86 len:2175 cov:291 mult:22.3846 [2019-09-06 08:59:00] DEBUG: 77 len:268018 cov:11 mult:0.846154 [2019-09-06 08:59:00] DEBUG: 74 len:34420 cov:8 mult:0.615385 [2019-09-06 08:59:00] DEBUG: -75 len:120396 cov:7 mult:0.538462 [2019-09-06 08:59:00] DEBUG: -43 len:837 cov:449 mult:34.5385 [2019-09-06 08:59:00] DEBUG: -42 len:676 cov:284 mult:21.8462 [2019-09-06 08:59:00] DEBUG: 41 len:637 cov:857 mult:65.9231 [2019-09-06 08:59:00] DEBUG: -40 len:831 cov:292 mult:22.4615 [2019-09-06 08:59:00] DEBUG: 44 len:729 cov:262 mult:20.1538 [2019-09-06 08:59:00] DEBUG: 35 len:667 cov:284 mult:21.8462 [2019-09-06 08:59:00] DEBUG: 82 len:5480 cov:297 mult:22.8462 [2019-09-06 08:59:00] DEBUG: 33 len:661 cov:279 mult:21.4615 [2019-09-06 08:59:00] DEBUG: 78 len:223186 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: 87 len:661754 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: -35 len:667 cov:284 mult:21.8462 [2019-09-06 08:59:00] DEBUG: -38 len:606 cov:832 mult:64 [2019-09-06 08:59:00] DEBUG: -82 len:5480 cov:297 mult:22.8462 [2019-09-06 08:59:00] DEBUG: 36 len:737 cov:419 mult:32.2308 [2019-09-06 08:59:00] DEBUG: -78 len:223186 cov:12 mult:0.923077 [2019-09-06 08:59:00] DEBUG: -41 len:637 cov:857 mult:65.9231 [2019-09-06 08:59:00] DEBUG: -44 len:729 cov:262 mult:20.1538 [2019-09-06 08:59:00] DEBUG: -34 len:827 cov:258 mult:19.8462 [2019-09-06 08:59:00] DEBUG: -36 len:737 cov:419 mult:32.2308 [2019-09-06 08:59:00] DEBUG: 43 len:837 cov:449 mult:34.5385 [2019-09-06 08:59:00] DEBUG: -28 len:125 cov:0 mult:0 [2019-09-06 08:59:00] DEBUG: 37 len:619 cov:274 mult:21.0769 [2019-09-06 08:59:00] DEBUG: 51 len:17473 cov:1 mult:0.0769231 [2019-09-06 08:59:00] DEBUG: 38 len:606 cov:832 mult:64 [2019-09-06 08:59:00] DEBUG: 27 len:921 cov:286 mult:22 [2019-09-06 08:59:00] DEBUG: -27 len:921 cov:286 mult:22 [2019-09-06 08:59:00] DEBUG: 28 len:125 cov:0 mult:0 [2019-09-06 08:59:00] DEBUG: 75 len:120396 cov:7 mult:0.538462 [2019-09-06 08:59:00] DEBUG: 42 len:676 cov:284 mult:21.8462 [2019-09-06 08:59:00] DEBUG: 40 len:831 cov:292 mult:22.4615 [2019-09-06 08:59:00] DEBUG: -33 len:661 cov:279 mult:21.4615 [2019-09-06 08:59:00] DEBUG: 34 len:827 cov:258 mult:19.8462 [2019-09-06 08:59:00] DEBUG: -37 len:619 cov:274 mult:21.0769 [2019-09-06 08:59:00] DEBUG: -90 len:411394 cov:13 mult:1 [2019-09-06 08:59:00] DEBUG: 39 len:623 cov:508 mult:39.0769 [2019-09-06 08:59:00] DEBUG: -39 len:623 cov:508 mult:39.0769 [2019-09-06 08:59:00] DEBUG: Unique coverage threshold 21 [2019-09-06 08:59:00] DEBUG: Read coverage cutoff: 1 [2019-09-06 08:59:00] DEBUG: Low coverage: -28 0 [2019-09-06 08:59:00] DEBUG: Removed 1 unsupported edges [2019-09-06 08:59:00] DEBUG: Unrolled 1 heterozygous loops [2019-09-06 08:59:00] DEBUG: Removed 0 heterozygous loops [2019-09-06 08:59:00] DEBUG: Popped 0 heterozygous bulges [2019-09-06 08:59:00] DEBUG: Tip len: 12430 cov:212 local:298 [2019-09-06 08:59:00] DEBUG: Tip len: 8935 cov:187 local:239 [2019-09-06 08:59:00] DEBUG: 0 tips clipped [2019-09-06 08:59:00] INFO: Resolving repeats [2019-09-06 08:59:00] DEBUG: Finding repeats [2019-09-06 08:59:00] DEBUG: Cov: 86 -> 36 -> 27 3833 314 [2019-09-06 08:59:00] DEBUG: Cov: -85 2235 248 [2019-09-06 08:59:00] DEBUG: Cov: 83 12430 212 [2019-09-06 08:59:00] DEBUG: Cov: -81 8935 187 [2019-09-06 08:59:00] DEBUG: Cov: 76 5155 62 [2019-09-06 08:59:00] DEBUG: Cov: -43 837 449 [2019-09-06 08:59:00] DEBUG: Cov: -42 676 284 [2019-09-06 08:59:00] DEBUG: Cov: 41 637 857 [2019-09-06 08:59:00] DEBUG: Cov: -40 831 292 [2019-09-06 08:59:00] DEBUG: Cov: 44 729 262 [2019-09-06 08:59:00] DEBUG: Cov: 35 667 284 [2019-09-06 08:59:00] DEBUG: Cov: 82 5480 297 [2019-09-06 08:59:00] DEBUG: Cov: 33 661 279 [2019-09-06 08:59:00] DEBUG: Cov: -38 606 832 [2019-09-06 08:59:00] DEBUG: Cov: -34 827 258 [2019-09-06 08:59:00] DEBUG: Cov: 37 619 274 [2019-09-06 08:59:00] DEBUG: Cov: 39 623 508 [2019-09-06 08:59:00] DEBUG: Repeat detection iteration 1 [2019-09-06 08:59:00] DEBUG: Writing Dot [2019-09-06 08:59:00] DEBUG: Writing FASTA [2019-09-06 08:59:00] DEBUG: Total unique edges: 12 [2019-09-06 08:59:00] DEBUG: Connection 90 -74 6 1 [2019-09-06 08:59:00] DEBUG: Connection -89 -80 9 1 [2019-09-06 08:59:00] DEBUG: Connection 84 77 6 1 [2019-09-06 08:59:00] DEBUG: Connection 87 -78 6 1 [2019-09-06 08:59:00] DEBUG: Connection 75 74 4 1 [2019-09-06 08:59:00] DEBUG: Connection -80 87 6 1 [2019-09-06 08:59:00] DEBUG: Connection -75 84 6 1 [2019-09-06 08:59:00] DEBUG: Resolved: 7 links: 86 [2019-09-06 08:59:00] DEBUG: Unresolved: 0 [2019-09-06 08:59:00] DEBUG: Finding repeats [2019-09-06 08:59:00] DEBUG: Cov: 86 -> 36 -> 27 3833 314 [2019-09-06 08:59:00] DEBUG: Cov: -85 2235 248 [2019-09-06 08:59:00] DEBUG: Cov: 83 12430 212 [2019-09-06 08:59:00] DEBUG: Cov: -81 8935 187 [2019-09-06 08:59:00] DEBUG: Cov: -43 837 449 [2019-09-06 08:59:00] DEBUG: Cov: -42 676 284 [2019-09-06 08:59:00] DEBUG: Cov: 41 637 857 [2019-09-06 08:59:00] DEBUG: Cov: -40 831 292 [2019-09-06 08:59:00] DEBUG: Cov: 44 729 262 [2019-09-06 08:59:00] DEBUG: Cov: 35 667 284 [2019-09-06 08:59:00] DEBUG: Cov: 82 5480 297 [2019-09-06 08:59:00] DEBUG: Cov: 33 661 279 [2019-09-06 08:59:00] DEBUG: Cov: -38 606 832 [2019-09-06 08:59:00] DEBUG: Cov: -34 827 258 [2019-09-06 08:59:00] DEBUG: Cov: 37 619 274 [2019-09-06 08:59:00] DEBUG: Cov: 39 623 508 [2019-09-06 08:59:00] DEBUG: Repeat detection iteration 1 [2019-09-06 08:59:00] DEBUG: Total unique edges: 19 [2019-09-06 08:59:00] DEBUG: Resolved: 0 links: 0 [2019-09-06 08:59:00] DEBUG: Unresolved: 0 [2019-09-06 08:59:00] DEBUG: Removed 0 simple and 0 double chimeric junctions [2019-09-06 08:59:00] DEBUG: Unrolled 0 heterozygous loops [2019-09-06 08:59:00] DEBUG: Removed 0 heterozygous loops [2019-09-06 08:59:00] DEBUG: Popped 0 heterozygous bulges [2019-09-06 08:59:00] DEBUG: Tip len: 12430 cov:212 local:298 [2019-09-06 08:59:00] DEBUG: Tip len: 8935 cov:187 local:239 [2019-09-06 08:59:00] DEBUG: 0 tips clipped [2019-09-06 08:59:00] DEBUG: Finding repeats [2019-09-06 08:59:00] DEBUG: Cov: 86 -> 36 -> 27 3833 314 [2019-09-06 08:59:00] DEBUG: Cov: -85 2235 248 [2019-09-06 08:59:00] DEBUG: Cov: 83 12430 212 [2019-09-06 08:59:00] DEBUG: Cov: -81 8935 187 [2019-09-06 08:59:00] DEBUG: Cov: -43 837 449 [2019-09-06 08:59:00] DEBUG: Cov: -42 676 284 [2019-09-06 08:59:00] DEBUG: Cov: 41 637 857 [2019-09-06 08:59:00] DEBUG: Cov: -40 831 292 [2019-09-06 08:59:00] DEBUG: Cov: 44 729 262 [2019-09-06 08:59:00] DEBUG: Cov: 35 667 284 [2019-09-06 08:59:00] DEBUG: Cov: 82 5480 297 [2019-09-06 08:59:00] DEBUG: Cov: 33 661 279 [2019-09-06 08:59:00] DEBUG: Cov: -38 606 832 [2019-09-06 08:59:00] DEBUG: Cov: -34 827 258 [2019-09-06 08:59:00] DEBUG: Cov: 37 619 274 [2019-09-06 08:59:00] DEBUG: Cov: 39 623 508 [2019-09-06 08:59:00] DEBUG: Repeat detection iteration 1 [2019-09-06 08:59:00] DEBUG: Writing Dot [2019-09-06 08:59:00] DEBUG: Writing FASTA [2019-09-06 08:59:00] DEBUG: Peak RAM usage: 10 Gb -----------End assembly log------------ [2019-09-06 08:59:01] root: INFO: >>>STAGE: trestle [2019-09-06 08:59:01] root: DEBUG: Finding unbridged repeats [2019-09-06 08:59:01] root: INFO: Simple unbridged repeats: 0 [2019-09-06 08:59:01] root: INFO: Resolved: 0 [2019-09-06 08:59:01] root: INFO: >>>STAGE: contigger [2019-09-06 08:59:01] root: INFO: Generating contigs [2019-09-06 08:59:01] root: DEBUG: -----Begin contigger analyser log------ [2019-09-06 08:59:01] root: DEBUG: Running: flye-contigger -l /data/user2/work/84/output/flye.log -t 8 -v 5000 -k 15 /data/user2/work/84/output/21-trestle/repeat_graph_edges.fasta all.fastq /data/user2/work/84/output/30-contigger /root/miniconda2/lib/python2.7/site-packages/flye/config/bin_cfg/asm_raw_reads.cfg /data/user2/work/84/output/21-trestle/repeat_graph_dump /data/user2/work/84/output/20-repeat/read_alignment_dump [2019-09-06 08:59:01] DEBUG: Build date: Apr 7 2019 02:34:49 [2019-09-06 08:59:01] DEBUG: Total RAM: 204 Gb [2019-09-06 08:59:01] DEBUG: Available RAM: 198 Gb [2019-09-06 08:59:01] DEBUG: Total CPUs: 32 [2019-09-06 08:59:01] DEBUG: Parameters: [2019-09-06 08:59:01] DEBUG: big_genome_threshold=29000000 [2019-09-06 08:59:01] DEBUG: low_cutoff_warning=1 [2019-09-06 08:59:01] DEBUG: hard_min_coverage_rate=10 [2019-09-06 08:59:01] DEBUG: assemble_kmer_sample=1 [2019-09-06 08:59:01] DEBUG: repeat_graph_kmer_sample=1 [2019-09-06 08:59:01] DEBUG: read_align_kmer_sample=1 [2019-09-06 08:59:01] DEBUG: maximum_jump=1500 [2019-09-06 08:59:01] DEBUG: maximum_overhang=1500 [2019-09-06 08:59:01] DEBUG: repeat_kmer_rate=100 [2019-09-06 08:59:01] DEBUG: assemble_ovlp_divergence=0.30 [2019-09-06 08:59:01] DEBUG: repeat_graph_ovlp_divergence=0.15 [2019-09-06 08:59:01] DEBUG: repeat_graph_ovlp_end_adjust=0.00 [2019-09-06 08:59:01] DEBUG: read_align_ovlp_divergence=0.25 [2019-09-06 08:59:01] DEBUG: max_coverage_drop_rate=5 [2019-09-06 08:59:01] DEBUG: chimera_window=100 [2019-09-06 08:59:01] DEBUG: min_reads_in_disjointig=4 [2019-09-06 08:59:01] DEBUG: max_inner_reads=10 [2019-09-06 08:59:01] DEBUG: max_inner_fraction=0.25 [2019-09-06 08:59:01] DEBUG: add_unassembled_reads=0 [2019-09-06 08:59:01] DEBUG: max_separation=500 [2019-09-06 08:59:01] DEBUG: tip_length_threshold=100000 [2019-09-06 08:59:01] DEBUG: unique_edge_length=50000 [2019-09-06 08:59:01] DEBUG: min_repeat_res_support=0.51 [2019-09-06 08:59:01] DEBUG: out_paths_ratio=5 [2019-09-06 08:59:01] DEBUG: graph_cov_drop_rate=10 [2019-09-06 08:59:01] DEBUG: coverage_estimate_window=100 [2019-09-06 08:59:01] DEBUG: extend_contigs_with_repeats=1 [2019-09-06 08:59:01] DEBUG: Running with k-mer size: 15 [2019-09-06 08:59:01] DEBUG: Selected minimum overlap 5000 [2019-09-06 08:59:01] INFO: Reading sequences [2019-09-06 08:59:01] DEBUG: Building positional index [2019-09-06 08:59:01] DEBUG: Total sequence: 65381103 bp [2019-09-06 08:59:01] DEBUG: UPath 1: 86 -> 36 -> 27 [2019-09-06 08:59:01] DEBUG: UPath 2: -89 -> 92 -> -80 -> 96 -> 87 -> 94 -> -78 -> -77 -> 93 -> -84 -> -97 -> 75 -> -95 -> 74 -> -91 -> -90 [2019-09-06 08:59:01] DEBUG: UPath 3: -40 [2019-09-06 08:59:01] DEBUG: UPath 4: 85 [2019-09-06 08:59:01] DEBUG: UPath 5: -44 [2019-09-06 08:59:01] DEBUG: UPath 6: 83 [2019-09-06 08:59:01] DEBUG: UPath 7: 82 [2019-09-06 08:59:01] DEBUG: UPath 8: -50 [2019-09-06 08:59:01] DEBUG: UPath 9: -35 [2019-09-06 08:59:01] DEBUG: UPath 10: -67 [2019-09-06 08:59:01] DEBUG: UPath 11: -51 [2019-09-06 08:59:01] DEBUG: UPath 12: 33 [2019-09-06 08:59:01] DEBUG: UPath 13: -39 [2019-09-06 08:59:01] DEBUG: UPath 14: -41 [2019-09-06 08:59:01] DEBUG: UPath 15: 42 [2019-09-06 08:59:01] DEBUG: UPath 16: 43 [2019-09-06 08:59:01] DEBUG: UPath 17: 81 [2019-09-06 08:59:01] DEBUG: UPath 18: -37 [2019-09-06 08:59:01] DEBUG: UPath 19: -34 [2019-09-06 08:59:01] DEBUG: UPath 20: -38 [2019-09-06 08:59:01] DEBUG: Final graph contiain 20 egdes [2019-09-06 08:59:01] DEBUG: Extending contigs into repeats [2019-09-06 08:59:02] DEBUG: Covered 0 repetitive contigs [2019-09-06 08:59:02] INFO: Generated 20 contigs [2019-09-06 08:59:02] DEBUG: Writing FASTA [2019-09-06 08:59:02] DEBUG: Generating scaffold connections [2019-09-06 08:59:02] DEBUG: Writing Dot [2019-09-06 08:59:02] DEBUG: Writing FASTA [2019-09-06 08:59:02] DEBUG: Writing Gfa [2019-09-06 08:59:02] DEBUG: Peak RAM usage: 0 Gb -----------End assembly log------------ [2019-09-06 08:59:02] root: INFO: >>>STAGE: polishing [2019-09-06 08:59:02] root: INFO: Polishing genome (1/1) [2019-09-06 08:59:02] root: INFO: Running minimap2 [2019-09-06 08:59:10] root: INFO: Separating alignment into bubbles [2019-09-06 08:59:42] root: DEBUG: Generated 221275 bubbles [2019-09-06 08:59:42] root: DEBUG: Split 1 long bubbles [2019-09-06 08:59:42] root: DEBUG: Skipped 1 empty bubbles [2019-09-06 08:59:42] root: DEBUG: Skipped 0 bubbles with long branches [2019-09-06 08:59:42] root: INFO: Alignment error rate: 0.161113900819 [2019-09-06 08:59:42] root: INFO: Correcting bubbles [2019-09-06 09:01:14] root: DEBUG: Generating polished GFA [2019-09-06 09:01:18] root: DEBUG: 0 sequences remained unpolished [2019-09-06 09:01:18] root: INFO: >>>STAGE: finalize [2019-09-06 09:01:18] root: DEBUG: ---Output dir contents:---- [2019-09-06 09:01:18] root: DEBUG: /data/user2/work/84/output: total 9348 drwxr-xr-x 2 34 Sep 6 08:56 00-assembly drwxr-xr-x 2 37 Sep 6 08:57 10-consensus drwxr-xr-x 2 198 Sep 6 08:59 20-repeat drwxr-xr-x 2 139 Sep 6 08:59 21-trestle drwxr-xr-x 2 177 Sep 6 08:59 30-contigger drwxr-xr-x 2 129 Sep 6 09:01 40-polishing -rw-r--r-- 1 4767354 Sep 6 09:01 assembly.fasta -rw-r--r-- 1 4700500 Sep 6 09:01 assembly_graph.gfa -rw-r--r-- 1 3604 Sep 6 08:59 assembly_graph.gv -rw-r--r-- 1 92245 Sep 6 09:01 flye.log -rw-r--r-- 1 109 Sep 6 09:01 params.json /data/user2/work/84/output/00-assembly: total 7140 -rw-r--r-- 1 7309685 Sep 6 08:56 draft_assembly.fasta /data/user2/work/84/output/10-consensus: total 6668 -rw-r--r-- 1 6824781 Sep 6 08:57 consensus.fasta /data/user2/work/84/output/20-repeat: total 12328 -rw-r--r-- 1 5695 Sep 6 08:59 graph_after_rr.gv -rw-r--r-- 1 4500129 Sep 6 08:59 graph_before_rr.fasta -rw-r--r-- 1 5035 Sep 6 08:59 graph_before_rr.gv -rw-r--r-- 1 2728055 Sep 6 08:59 read_alignment_dump -rw-r--r-- 1 94868 Sep 6 08:59 repeat_graph_dump -rw-r--r-- 1 5271919 Sep 6 08:59 repeat_graph_edges.fasta /data/user2/work/84/output/21-trestle: total 5272 -rw-r--r-- 1 94868 Sep 6 08:59 repeat_graph_dump -rw-r--r-- 1 5293527 Sep 6 08:59 repeat_graph_edges.fasta -rw-r--r-- 1 0 Sep 6 08:59 repeats_dump -rw-r--r-- 1 0 Sep 6 08:59 resolved_copies.fasta -rw-r--r-- 1 168 Sep 6 08:59 trestle_summary.txt /data/user2/work/84/output/30-contigger: total 13240 -rw-r--r-- 1 4532551 Sep 6 08:59 contigs.fasta -rw-r--r-- 1 714 Sep 6 08:59 contigs_stats.txt -rw-r--r-- 1 4532511 Sep 6 08:59 graph_final.fasta -rw-r--r-- 1 4480837 Sep 6 08:59 graph_final.gfa -rw-r--r-- 1 3604 Sep 6 08:59 graph_final.gv -rw-r--r-- 1 0 Sep 6 08:59 scaffolds_links.txt /data/user2/work/84/output/40-polishing: total 13916 -rw-r--r-- 1 300 Sep 6 09:01 contigs_stats.txt -rw-r--r-- 1 4767354 Sep 6 09:01 polished_1.fasta -rw-r--r-- 1 4774487 Sep 6 09:01 polished_edges.fasta -rw-r--r-- 1 4700500 Sep 6 09:01 polished_edges.gfa [2019-09-06 09:01:18] root: DEBUG: -------------------------- [2019-09-06 09:01:18] root: INFO: Assembly statistics: Total length: 4689029 Fragments: 16 Fragments N50: 4588463 Largest frg: 4588463 Scaffolds: 0 Mean coverage: 14 [2019-09-06 09:01:18] root: INFO: Final assembly: /data/user2/work/84/output/assembly.fasta