##FastQC 0.11.8 >>Basic Statistics pass #Measure Value Filename input.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9349 Sequences flagged as poor quality 0 Sequence length 18-309435 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 3.1040753021713554 3.0 2.0 3.0 2.0 4.0 2 4.475451919991443 4.0 3.0 6.0 2.0 8.0 3 6.400791528505723 6.0 3.0 9.0 2.0 12.0 4 6.852283666702321 6.0 4.0 9.0 3.0 13.0 5 7.230719863086962 6.0 4.0 9.0 3.0 13.0 6 8.797197561236496 7.0 5.0 11.0 3.0 16.0 7 9.923842122152102 8.0 6.0 13.0 4.0 18.0 8 10.214247513103006 8.0 5.0 13.0 4.0 20.0 9 11.273077334474275 9.0 6.0 15.0 4.0 22.0 10-4999 17.274243670586742 15.936673346693388 9.991583166332665 23.358917835671342 6.6334669338677354 30.67434869739479 5000-9999 17.46785766172009 16.018 10.0894 23.707 6.892 30.8516 10000-14999 17.494537163417245 16.0556 10.1894 23.6846 6.8746 30.8136 15000-19999 17.54328688881565 16.1224 10.2808 23.7184 6.8608 30.839 20000-24999 17.58733120591988 16.1858 10.3738 23.7576 6.8492 30.8642 25000-29999 17.569788571494904 16.1628 10.3634 23.7206 6.82 30.815 30000-34999 17.570655449359897 16.1734 10.3626 23.7148 6.784 30.8028 35000-39999 17.675872753704972 16.2808 10.5046 23.8152 6.8472 30.8702 40000-44999 17.763140739281244 16.392 10.5884 23.9076 6.8936 30.8854 45000-49999 17.762372658172694 16.3824 10.5704 23.888 6.874 30.8686 50000-54999 17.746326369234033 16.382 10.5612 23.8312 6.8504 30.8082 55000-59999 17.805055348721147 16.4222 10.5996 23.8882 6.8576 30.8318 60000-64999 17.992058209649386 16.6148 10.764 24.1106 6.9858 30.9498 65000-69999 17.978968348421585 16.6104 10.7592 24.067 6.9624 30.849 70000-74999 17.713405908873664 16.2748 10.4762 23.6868 6.7476 30.5158 75000-79999 17.852066758533983 16.4498 10.6172 23.809 6.8528 30.5392 80000-84999 17.862249132213076 16.344370860927153 10.554746136865342 23.760706401766004 6.856953642384106 30.4916114790287 85000-89999 18.012859377625343 NaN NaN NaN NaN NaN 90000-94999 18.005107581778805 NaN NaN NaN NaN NaN 95000-99999 17.968044814690742 NaN NaN NaN NaN NaN 100000-104999 17.947299919313767 NaN NaN NaN NaN NaN 105000-109999 18.024560724477915 NaN NaN NaN NaN NaN 110000-114999 17.701630879486697 NaN NaN NaN NaN NaN 115000-119999 17.649805852561933 NaN NaN NaN NaN NaN 120000-124999 17.648836944997853 NaN NaN NaN NaN NaN 125000-129999 17.940682813346065 NaN NaN NaN NaN NaN 130000-134999 17.64192571428568 NaN NaN NaN NaN NaN 135000-139999 18.131199924320583 NaN NaN NaN NaN NaN 140000-144999 18.273635294117682 NaN NaN NaN NaN NaN 145000-149999 17.788935294117657 NaN NaN NaN NaN NaN 150000-154999 17.735339999999987 NaN NaN NaN NaN NaN 155000-159999 17.738988351648338 NaN NaN NaN NaN NaN 160000-164999 17.67735384615383 NaN NaN NaN NaN NaN 165000-169999 17.903464102564072 NaN NaN NaN NaN NaN 170000-174999 17.783083333333327 NaN NaN NaN NaN NaN 175000-179999 17.245852727272666 NaN NaN NaN NaN NaN 180000-184999 17.63447111111107 NaN NaN NaN NaN NaN 185000-189999 17.64624444444442 NaN NaN NaN NaN NaN 190000-194999 18.092133333333333 NaN NaN NaN NaN NaN 195000-199999 18.99235952380957 NaN NaN NaN NaN NaN 200000-204999 18.299099999999964 NaN NaN NaN NaN NaN 205000-209999 18.221300000000006 NaN NaN NaN NaN NaN 210000-214999 18.372100000000035 NaN NaN NaN NaN NaN 215000-219999 18.143233333333335 NaN NaN NaN NaN NaN 220000-224999 17.782996666666644 NaN NaN NaN NaN NaN 225000-229999 18.429216666666676 NaN NaN NaN NaN NaN 230000-234999 17.845466666666717 NaN NaN NaN NaN NaN 235000-239999 17.20653333333333 NaN NaN NaN NaN NaN 240000-244999 17.54856666666667 NaN NaN NaN NaN NaN 245000-249999 18.1506 NaN NaN NaN NaN NaN 250000-254999 17.848 NaN NaN NaN NaN NaN 255000-259999 16.8378 NaN NaN NaN NaN NaN 260000-264999 18.1554 NaN NaN NaN NaN NaN 265000-269999 19.1362 NaN NaN NaN NaN NaN 270000-274999 20.0036 NaN NaN NaN NaN NaN 275000-279999 19.76 NaN NaN NaN NaN NaN 280000-284999 18.2366 NaN NaN NaN NaN NaN 285000-289999 18.7148 NaN NaN NaN NaN NaN 290000-294999 19.5824 NaN NaN NaN NaN NaN 295000-299999 19.5486 NaN NaN NaN NaN NaN 300000-304999 18.7322 NaN NaN NaN NaN NaN 305000-309435 19.39517583408476 NaN NaN NaN NaN NaN >>END_MODULE >>Per sequence quality scores fail #Quality Count 8 24.0 9 234.0 10 211.0 11 231.0 12 304.0 13 384.0 14 477.0 15 745.0 16 1007.0 17 1408.0 18 1755.0 19 1663.0 20 799.0 21 103.0 22 3.0 23 0.0 24 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 10.407530217135523 6.203872071879346 72.2109316504439 11.177666060541235 2 24.826184618675796 2.7061717830784042 54.775911862231254 17.691731736014546 3 60.723071986308696 14.536314044282811 9.487645737512034 15.25296823189646 4 37.72595999572147 12.151032195956788 41.82265482939352 8.300352978928228 5 19.5101080329447 9.66948336720505 62.306128997753774 8.514279602096481 6 33.586479837415766 28.17413627125896 27.16868114236817 11.070702748957109 7 25.350304845438014 11.733875280778692 44.86041287838272 18.055406995400578 8 25.221948871537066 17.028559204192963 36.59214889292973 21.15734303134025 9 25.93860305915071 11.16696972938282 30.95518237244625 31.939244839020215 10-4999 24.686497102244463 25.14638051013886 25.56693106278964 24.600191324827037 5000-9999 24.67802995416617 25.516397427282943 25.21192774117354 24.59364487737735 10000-14999 24.691056025665436 25.509491541741436 25.12132420304882 24.678128229544306 15000-19999 24.63535047850757 25.440278157405928 25.215675259631066 24.70869610445544 20000-24999 24.807821189781652 25.309448526757528 25.076467265154562 24.806263018306264 25000-29999 24.91416144069521 25.285186575093483 25.001785948429045 24.798866035782268 30000-34999 24.941854724421358 25.25277142500016 25.035372303842813 24.770001546735674 35000-39999 24.93411859246803 25.277550192573738 25.109290351903933 24.679040863054293 40000-44999 24.99834170235237 25.36906814009643 24.91999252258515 24.712597634966045 45000-49999 25.043194599352386 25.29950304165867 24.965543188453506 24.691759170535434 50000-54999 25.24865498395425 25.239407281286912 24.763183387190818 24.74875434756802 55000-59999 25.050284908187443 25.135479985100766 25.151092478265348 24.663142628446437 60000-64999 25.000145525667815 25.223672951435173 25.16080586293811 24.615375659958904 65000-69999 24.99723739099205 25.349700256922635 25.100950337498734 24.552112014586573 70000-74999 25.053223648010654 25.2681561589305 25.1043074812002 24.57431271185865 75000-79999 24.893715755794545 25.61128705294266 25.219366446581077 24.275630744681717 80000-84999 25.03836141555943 25.446560275200248 24.928518813328807 24.586559495911516 85000-89999 24.91715181370354 25.387486077141247 25.196872093423817 24.498490015731395 90000-94999 25.38736857386944 24.91582585584026 24.990647317315585 24.70615825297471 95000-99999 25.336728539837832 25.36608558856096 24.737780926214885 24.55940494538632 100000-104999 25.242889155752085 25.180039131622166 24.902825632334842 24.674246080290906 105000-109999 25.208712657630315 25.016746465259516 24.965045968485175 24.80949490862499 110000-114999 25.300209406128378 24.693295366564648 24.978046131702424 25.02844909560455 115000-119999 25.359174794415758 25.11892809332568 24.787841843564735 24.734055268693822 120000-124999 25.226991925983395 25.29882481395282 24.518001321725137 24.95618193833865 125000-129999 24.87438739017175 25.709744359574376 25.34858051128085 24.06728773897302 130000-134999 24.906459378716246 25.940965474567584 25.122062051029186 24.030513095686977 135000-139999 25.313517031894122 25.145367758732913 24.7667606856151 24.774354523757864 140000-144999 25.231764705882355 25.54705882352941 24.783529411764707 24.43764705882353 145000-149999 23.970835404682433 25.95104514357408 25.563998769026817 24.514120682716666 150000-154999 24.526069333601875 25.27881976788097 24.719921452128595 25.47518944638856 155000-159999 25.139023481177787 24.699217294073797 24.91539321654864 25.24636600819978 160000-164999 24.672307692307694 25.13230769230769 24.69230769230769 25.50307692307692 165000-169999 25.161153796067197 25.056973564266183 25.011394712853235 24.770477926813385 170000-174999 25.006666666666668 25.124999999999996 24.186666666666667 25.68166666666667 175000-179999 25.405962544485718 24.752533011804516 24.575562513370024 25.265941930339746 180000-184999 25.598437808543785 25.36805845053426 24.14156263027404 24.891941110647913 185000-189999 25.27333333333333 23.986666666666668 25.346666666666668 25.393333333333334 190000-194999 25.300502658993224 24.67400014569826 25.05281561885335 24.972681576455162 195000-199999 25.04430660168365 25.177226406734604 25.479065130704477 24.29940186087727 200000-204999 25.230000000000004 25.313333333333333 24.703333333333333 24.753333333333334 205000-209999 25.12333333333333 24.246666666666666 25.483333333333334 25.14666666666667 210000-214999 24.48 25.580000000000002 25.833333333333336 24.10666666666667 215000-219999 24.903333333333332 24.83 26.25 24.016666666666666 220000-224999 24.15581330905348 25.84220735521159 26.230157159257356 23.771822176477574 225000-229999 25.09017610863569 25.668364099299808 25.811584977721196 23.429874814343304 230000-234999 24.773333333333333 26.406666666666666 25.033333333333335 23.78666666666667 235000-239999 26.406666666666666 24.96666666666667 24.393333333333334 24.233333333333334 240000-244999 25.25531327629037 24.70328457079768 24.500874045450363 25.540528107461586 245000-249999 25.8 25.540000000000003 24.4 24.26 250000-254999 24.58 26.889999999999997 25.740000000000002 22.79 255000-259999 25.169999999999998 25.230000000000004 27.21 22.39 260000-264999 22.875912408759124 27.416058394160586 27.59124087591241 22.11678832116788 265000-269999 22.259999999999998 28.18 27.139999999999997 22.42 270000-274999 22.46 28.98 28.04 20.52 275000-279999 21.68 25.72 28.860000000000003 23.74 280000-284999 24.54 25.5 27.3 22.66 285000-289999 24.92 26.740000000000002 26.14 22.2 290000-294999 25.740000000000002 25.380000000000003 24.740000000000002 24.14 295000-299999 26.619999999999997 23.580000000000002 23.84 25.96 300000-304999 26.86 22.220000000000002 24.82 26.1 305000-309435 27.727682596934173 23.9404869251578 24.887285843101896 23.44454463480613 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 2.0 8 2.5 9 2.5 10 3.0 11 4.0 12 6.0 13 6.0 14 4.333333333333333 15 2.833333333333333 16 2.833333333333333 17 2.5 18 1.5 19 1.0 20 0.5 21 0.0 22 0.5 23 1.5 24 1.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 1.0928571428571427 31 1.0492424242424243 32 0.7294372294372294 33 1.27577295200246 34 1.6876297821419772 35 3.391866140395551 36 4.450964336521839 37 5.773064533177732 38 8.465740792916874 39 7.307451408953984 40 9.504580737139193 41 10.30610691130189 42 12.744869664712398 43 13.708011952555523 44 14.241486279548084 45 16.74459981681413 46 20.386009709946638 47 26.014118614504227 48 30.423593510860282 49 28.553994287982025 50 35.17919402869111 51 33.940283452635114 52 34.68124024693415 53 31.241363661312562 54 26.99867931091156 55 23.353203804278543 56 22.91055688743741 57 15.28587148931372 58 10.333593145621238 59 4.893729925987989 60 0.5703463203463204 61 0.6873015873015872 62 0.14285714285714285 63 0.5 64 1.0 65 2.0 66 2.0 67 0.5 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.3333333333333333 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-4999 0.0 5000-9999 0.0 10000-14999 0.0 15000-19999 0.0 20000-24999 0.0 25000-29999 0.0 30000-34999 0.0 35000-39999 0.0 40000-44999 0.0 45000-49999 0.0 50000-54999 0.0 55000-59999 0.0 60000-64999 0.0 65000-69999 0.0 70000-74999 0.0 75000-79999 0.0 80000-84999 0.0 85000-89999 0.0 90000-94999 0.0 95000-99999 0.0 100000-104999 0.0 105000-109999 0.0 110000-114999 0.0 115000-119999 0.0 120000-124999 0.0 125000-129999 0.0 130000-134999 0.0 135000-139999 0.0 140000-144999 0.0 145000-149999 0.0 150000-154999 0.0 155000-159999 0.0 160000-164999 0.0 165000-169999 0.0 170000-174999 0.0 175000-179999 0.0 180000-184999 0.0 185000-189999 0.0 190000-194999 0.0 195000-199999 0.0 200000-204999 0.0 205000-209999 0.0 210000-214999 0.0 215000-219999 0.0 220000-224999 0.0 225000-229999 0.0 230000-234999 0.0 235000-239999 0.0 240000-244999 0.0 245000-249999 0.0 250000-254999 0.0 255000-259999 0.0 260000-264999 0.0 265000-269999 0.0 270000-274999 0.0 275000-279999 0.0 280000-284999 0.0 285000-289999 0.0 290000-294999 0.0 295000-299999 0.0 300000-304999 0.0 305000-309435 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 0-9999 6464.0 10000-19999 1552.0 20000-29999 557.0 30000-39999 259.0 40000-49999 180.0 50000-59999 108.0 60000-69999 65.0 70000-79999 49.0 80000-89999 37.0 90000-99999 17.0 100000-109999 20.0 110000-119999 10.0 120000-129999 10.0 130000-139999 4.0 140000-149999 1.0 150000-159999 3.0 160000-169999 1.0 170000-179999 2.0 180000-189999 1.0 190000-199999 3.0 200000-209999 0.0 210000-219999 0.0 220000-229999 3.0 230000-239999 0.0 240000-249999 1.0 250000-259999 0.0 260000-269999 1.0 270000-279999 0.0 280000-289999 0.0 290000-299999 0.0 300000-309436 1.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 99.95721467536634 #Duplication Level Percentage of deduplicated Percentage of total 1 99.95719636169075 99.9144293507327 2 0.042803638309256285 0.08557064926730132 3 0.0 0.0 4 0.0 0.0 5 0.0 0.0 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-4999 0.005255992785657037 0.003704060168684768 0.012160378088512539 0.01329003069782802 0.0 5000-9999 0.01243769387100319 0.010696331158413416 0.03758690769066552 0.032835597390097955 0.0 10000-14999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.042785324633653664 0.0 15000-19999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.043119050165796174 0.0 20000-24999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 25000-29999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 30000-34999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 35000-39999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 40000-44999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 45000-49999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 50000-54999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 55000-59999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 60000-64999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 65000-69999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 70000-74999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 75000-79999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 80000-84999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 85000-89999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 90000-94999 0.021392662316826832 0.010696331158413416 0.042785324633653664 0.053481655792066374 0.0 95000-99999 0.029851320996900604 0.010696331158413416 0.042785324633653664 0.06194031447214129 0.0 100000-104999 0.032088993475240815 0.010696331158413416 0.042785324633653664 0.06417798695048163 0.0 105000-109999 0.032088993475240815 0.010696331158413416 0.04587870360466695 0.06727136592148882 0.0 110000-114999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.07612364958819026 0.0 115000-119999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 120000-124999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 125000-129999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 130000-134999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 135000-139999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 140000-144999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 145000-149999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 150000-154999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 155000-159999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 160000-164999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 165000-169999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 170000-174999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 175000-179999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 180000-184999 0.032088993475240815 0.010696331158413416 0.053481655792066374 0.08557064926730733 0.0 185000-189999 0.032088993475240815 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