eDNA~HaCeD-Seq_with_UMI_using_Nanopore

Pipeline to analyze Nanopore sequenced data with UMI for HaCeD-Seq. Adapter and primer sequences must be specified as 5’->3’ orientation as follows. Forward_adapter (Foward_UMI) Foward_primer (D-loop) Reverse_primer (Reverse_UMI) Reverse_primer 5’---------->3’ 5’-------->3’ 3’<---------5’ 3’<---------5’

input_1:Nanopore FASTQ(.gz) files

input_1/fastq_runid_6d7597e92539e514bba241b5892ca714de4705c7_0_0.fastq

@56edc451-1cfa-4cca-b73f-69922d6275bf runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=853 ch=112 start_time=2021-06-24T06:39:27Z
CTTATATGTAGTTCTTGGTTTAGTTTACATATACTTACACAACACTCTCCGATCTGTCTCTCTTTTTGAGGAGAGGCGCCGGTCTTGTAACCCGGATGTCAGGAGGTTAAAGTCCCCCCTTTTGCTCAAAGAAGAGTGATTCTAACACCCACCACTAACTCCAAAGCTCATATTAAATTAAAACTATTCTCTGATAGTGCATATATGGTTATCTTACATATATGGTGTACCCCCATATAATGAAATGAAGTAGGACATTACATAAGGGTAACATATATATATTTTAGTACATATATATACATGTAAACTAGACATTTATGTATTAACACCATCTTATGTTATAAATGCATTCAGGTAACCATTGAATAAAAGGGATTACACAAAGCATAATAATTACTGGCTTACATAAGACTCTTTAATAAGACAGTTAGCGATTTAAGATCTAACAAGACAAAGAACCCCATATATACCAAGTCATCCACTACCCTAATTCCGGAAAACATTCGATGTGATAAGAACCTACCATAGTTGGTTTCTTAATGCATACATTATGATAAGTTAGGGACCAGAAATCGTGGGGGTTTCACTTACATCAATTATTCCTGGCATCTGGTTCCTATTTCAGGGCCATAAATCTATAATTCCCCTAACTTTCATCGACGCTTGCATCAGTTAATGCTTTTAATCCCAATTCAATTCGTTCCCCCACCATGCCGGGCGTTCACTCCCAAGCAAGCAACGGTTCTCTTTTTTCGTTTTCCTTTCACTTGACATCTCACAGTGCCAGCGGTAATCAAAATAAGTGAAAACATTCCTTGAATATAATGATAATTATGAATAACACAAACATCACAGAAGAATTGCATAATTGGATATCATGAGCATAATGTTGATATATTTCTCCTCGTTATCTTATCGCCCTGTTCGGTTTTTGCGTCAAACCCCCCTACCCCCTCACTCCTGAGATCACTAAGACTCCTGAAAACCCCCGGGAACAGGAAAACCTCTAGTGCATATAATTAAGCCCAAAATGTGTCTATTTACACTATTAAAATAATGCGTGACTCACGTAGCTAAATTGAGAGCATAACACTAAAAATGTAAGATAGGCCCTCAAAGACTCCGCAAGCACGAAGGCTTGGTCCTGACTCTCTTCTGACTTAGCTAAACTTATACATGCAAGTCTCCAGAGGCTAGTCGTTTCAAATCGAGAAGACGCACGTCTCACGA
+
"#%$%'''*'&&)()**./.,%%&&(***+*,++***/21((**+411(((---16;:<;?{967;5)''''*++*(&&&##%%(,))&%%''&%%%%+/*''():>?2//..33387:99::/((((*?ADGD@<<;:=@?@@@@A?=<<<<<=>?>?@<=005458---,+0)))67;;;>BD100000A>>>>>@?;654384'%%%%((('&(/79;{7-++++++*-+)0591--@@CFECEDB@@<<<====>=>===<<;=;<?@?>?AK{{{+5''&&(...157;{{{{D?>>>?D=<<<1000//664ABDBCCEGFFDBAB98967CBBBC??@:875550/+++-8:A?<:6677//-,+;52355==EABFDBAA?@A@CGEA55555CCCC@@>?A@@@>?@B9:9112269765/..)(((((++45577788,+*'%%&*-+)((...012{/,-,-55499::;?E<:;;210((('&&%(*79776,***+8...,,-,/--.,+*)'%%$%%&'(''))+)))-12(((((%'&&&/)('')'((+-789;;883)))*+-//12-.+++'&$$$%&%%&&&+,03@ACFEFFDDEC<;;::?////12<7,(%%%$&%%%&%',,161<=<;<934=;;<=88889<))&%&&&2479=???A2//01,&''&&&'&'&%%''.../)''&(03.-,,,-..../3--,+*./,(''(()&'**12-.../>F84/++)',...)36:?45<:;9999:*((()2//1223368+)****(&'%$%'&&(23/&&5589<>@C9:;:;>;:;;82..-*'***.*)))*,546;433@DEEDDCBDCCFFA<<5.,***)()((('/034CB@A>><7540/--('&&&$%%%$##%%&((((&&%%(*+896+((''().-/025890/./1:.+*3331-*,++''')3-)((),,,,*+.,,+++--+)(''&'/25----.:990//&&%%&&((())+334A@CEFEFEBB0...-/+,294,++../7778:10&&&&'*'(('''&*0)((()&%%&%&(751..15)&&&&&&&'246641111:9'&&&&.))()000**,,,(''((-/0(())*-+++,('&'%%%%))*/.098///33B?@80,,,,-6666=:732223695///2222('&&%%%$$#'&))&&&()01:444)(
@58829d67-0ea7-43fc-bba0-df91ccd878a0 runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=378 ch=427 start_time=2021-06-24T06:39:22Z
ATATGTCATATTTACTTCAGTTCAGTTACATCTGCTTACACGACGCTCTTCCGATATACTGTCTGACTTTTAATTACACTCTGGTTCGATGACTCCTTTCATGTGCATTGTGAGGACACTGTGGTTCTGTTTTCATGCTTCATCTCCCTTACAAACAGTAACTGGCAAGTCCATTAAGACATTTAGCATTGTCAGTTCTGAATCAAAAAAATAGGTCTTTTCTGTCTATTATAAGC

input_1/fastq_runid_6d7597e92539e514bba241b5892ca714de4705c7_1_0.fastq

@f31b88c8-39c2-4aa7-9531-e6274fdd49d9 runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=11942 ch=474 start_time=2021-06-24T10:07:55Z
TTTATGTGTTTAACGTATTTTTCGTTCAGTTACGTATTGTTAGACGTGTGCTCTTCCGATCTCAGTTCATGATCATGGAGACTTGCATGTGTAAATCTAGCTAAAGCTGACAGTAAAGTCAGGACCAAACCTTGTGCTTACAGTTTATTCAGGCCCATCTTAACATCTTCAGTGTTATGCTTTAATTAAGCTACGCTAGCATGTAAAATCTATAATAATTTATATACATGCACTTTGGGATAAAAAAAAACTTCTAGAGGCTTCCCTGTTCCGGGGGGTTTTTCAGGAATGACAGCGATCTTGGGAGTTTAAAGATAAGGATTTACGCATAAAGCCAAGAGGGAATCTTTAGATATGTTAGGAGAAGTAGTTACACCTTATGCTCTTGATATCCTAATATGTGGTTATCGATTACAATATCTTATCACCATGAATATTATTTACGGCGAGCAAATCTCTACCTTATTAGTTATATCTCATTGCGCACTCTGAAATGTCAAGTGAAAGGAAGACAAAAAAACCCTTACCCGCTGGAAAGAACACCGGCATGAGGATGACGAGGAGTATGTATTCCCACCATTAACTTATGTAAGGGCGTCGATGAAGTCCGAAAATAAAGCAATCAATGGCCCTGAAGTAGGAACCAAATGCCAGGAATAGTTCACTAAGTGAAACCCCCACGATTTTTGTTCCTCACCTTCAATAACCGTTATCACTTCGTTATCACCGGTTGGTAGGCTCTTCTTAGTTGATTTCTATCAATTGAACGAGATGAGGGTTTCTTGGTAGTCCATCACTGACCTTTGCAGGTTGGACTGGGAGATGCATATTGGATATTTTAATTTAAGTTCAGTTCTTTGGTTAATAAATTTGGGCCCATGTACTGTTATATGGGTGTGTATAACCTGAGTGGTTAATTCCAACTAATGGTGATAATACATACATGTTATTATAGCATGTAGTGTTATGTCATACAATATATATATGGTAATAGTACATATATGTCTTTAAAAAACTAAAATATTATATATGCGCTGTGAAATGGCAGAGAATAGTTTAATGTTAGAATCCTAGCTTTGGGAGTTCAGGGCAGGGCTGAGAGTGAACTTATTTTTTCTGAGCAGTAGGGAGGACTTTAACCTCCGGCATCCGGTTTACAACCAGTAGTAACACGCTCCGACAGATCGGAAGAGCGTCGTGTAAGCAATA
+
$%))**+))('%&'%%%$$%%)'%&%&)'''))***+.3/0//06567:9;<;;<;8444461.,-,-//)('&%%####'))+**:<<<=@AAABBC<>@CACDDBCBAAA?>>@BA@?>=>>>@>>=9/.,'*7754(&&$$%&%&&$$$%&'5559?=2000@>>>=>=>=>DDDCB@>1.9//6443466;;;5444780((()1''''(;;EDHJIFFD{DBA@???@<CC::5352365?BBBC<98242----->=/./,-2-'(...046/-,-,,121115222//.))*))*''%$%&'($$%$%%%%&&%&&'%'((%%$#%'010111-*'')&$('($(5@EGAB6()==?EE>>,,,,,6<<;<?9;ECC>>???EGF=::::CFDDB;:;;:>82222;<:9:5555555??8864--,*'%%%%')&*()))&&&&'''$$$$$0189>BG@?@??D?{.,*)))&%&((((-,--.;<>A@@D>>===C<54533430/4-,+,1507:<=<9:=>@ACDEGGA)'&%&)&$%$$%%%$$$$$$%%%*,++-/@@AAB=<???BBBBGFCA?<;4-+)'''%$$$%-31100/-)),)022212238////1>=:;;;=@G998878;21101=>????ABCDCDCDBC@>;<;DCEHB>>>>=;;:43<75420124?>@@@?AC==F?<766**('''&$#$&&*///1.038333336678?944*&&&&%$'()1/+,,:::::<<<74100./036<>..//.*+%%%$#$%&)()(((3??E@?@=<=?>=:887879;;=?>??>@A:9999>>==::==>82--.,++++-,-/-4=AAFFFIFDBA>?<966678?>====@CCCD><;>>?CCBA@@?@@??<==<<@ABDFD>>><?ACBA;;;<>GHGFCBAAAEDCCCABBBAAA????@?==<<=>>@BABCED=<=<;>{{{{Q?=>=:;11.**+,,/2C@0,++-,6=DJFED9;DGIDBBAAB{{{@<88879=CDDD;:;5..//0/.***0004<A@;6666332+)'''*%&&&'23-)))+'763.,-%$$$%%'''&(*'&'''(..0246?=<<76666ADD+++++=A@@@?AA??CAA=:67=54'$$$%&%&%%'-.12546789;;:;<<56666<:@966100116555...-
@bd5d7683-cb9e-4b36-9309-dde7b9acc7c3 runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=11396 ch=384 start_time=2021-06-24T10:08:00Z
TTATGTGGTCTACCTTGGTTCATTTACATATTGCTACGCTATTCCGATCTGTCCTACAGTAGTATGAAAGCATCGGTTTTATCGTAAAGAAAGCTTCTCCACCATCTCCATGTAGCCAAGTTGTTGCAGCTTCCTCCGTCTATTATGACGCGCACAGAACGTTCCTTCACAACTCCCTTGGTCTGGAACAAATTGTGCCTGTGATTTTGCTCAGCTTGTGTGACCTGCACACTCAAAACACGTTGAGCAACTAAACATTCATACCTGTCAGCGT

input_1/fastq_runid_6d7597e92539e514bba241b5892ca714de4705c7_2_0.fastq

@7bc0ff57-fc49-4d3d-b37f-4a9f477bd3ae runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=11932 ch=19 start_time=2021-06-24T10:08:41Z
GTCCATCTACAGTTCAGTTACGTATTGCTTACACGACGCTCTTCCGATCTGTCTCTCCTCAAAGTGAACCATCTTATGAGCCGGATGCCGGAGGTTAAAACATCCCTACTGCTCAAAGAAAGGAGATTTTAACTCCTACCCCAACTCCCAAAGCTAGGATTCTAAAATTAATTGTCTCTTTGTTTCAAAAAATGCATATATGTATTTACACCATAATTATATTAAACATATCAATGATATTCAGTAGTACATTAATATTTTATTGAGCATTTAATAGGAGTTAACCATTCTAACCAACACATATTAACTAAGGGTTACGCAAACCTAGAAATGTAATTAACATTAATTGAAATATGGACAAGCGAGATTTAACCAGCACAAAACTTGATTTAGTAATCTTCCAATCTACCAGGACTCAAAATCTCGTCAATATAGAATCTTTAAATATAGTAAGAACCGACCATCAGTTGATTTCTGAATGCCAACGGTTAATGATGGTGAGGTACAATTATAGAGTTCCCACTTAGTGAATTATTCCTGGCATTTGGTTCCTTCTTTCAGGGCCATTAATTGATATTCATTCCCCACTCTTTCATCGACGCTTGCATAGTTCCGTGGTCGCGTACGTTCGGACTCGTTACCCACCATGCCGAGCGTTCTCTCTCAGGGGTCAGGGGTTCTCTTTTTTTTTTTCCTTCATCTGGCATCCCAGAGTGCACACGGTATTAATTACTAAGGTTGAACATTTCCTCGCGCAAGGAATAAGTATGAATGATAAAAGAGGGTCTTGATGAGAGAATTGCATAACTGATTTCATGAGCATAAATTATGAATATAACTCCTAAAATCTCTAAGATACCCCTGGGGTTTTGCAGGTAAACCCCCCTACCCCCCAATACTCCTGGGCTCCGGTACACTCCCTGAAAACCCCGGAAACAGGAAACCTTCTACATAGTATAATTTAACCAAGGTGTCTATTTACACTATTAAAATAATGCATAAAGCTAGCGTAGCTTAATTAAAGCATAACACTGAAGATGTTAAGATGGGCCCTCAAAGACTACAAGCACAAAGGCTTGGTCCTGACTTTACTATCAACTTTAGCTAAACTTATACATGCAAGTCTCCGAGGCTAGTAATAAATCGGAAGAGCACACGTCTAGCAATAC
+
$&$%%&%%$$#$$$%*.//,****,,,,-802;;+++++++,-,039<89998837435522320+(('&&&&&))+''''(89;9::3.-/001--/44*)))()21---26/-,+*,,-*0......,'&&&%%'''(73.:;<:50))**,;6)))),24)((--200-,**&%&&'-+788459:@BJG@?>>?BGGBBAABDB@99320))&':;<=:<<8:6765*****-110/(&()(,.11182(((=;<;-)22----,**,')+)('&''+../0***&&**,75678:6333212:82--(('()-,0/0478=3222345,++*+00024/(*?@?=50*,BA>@@.,,++.,++,--++)(&&''&&%'&('%%''&&$$%'&%%%')***++---***99952115668.--*)*+*,*'&&()/0/(*(&'))))24<??>:9989=?=>:::;=??>73224A?<<:79--089@B762----.977755555*)'%$$')('(((((%%(((+7::;9722225BCCB>>>=<<=@?@=4*+1119:;<=><>?333+*)(()***+,./655:<5442--......3340'''$$$%%%('&'%%%%%++,--,-1-,,,-9;9.,,,,89:5333--.(('&(*%&'*,4465567=;?;;75325069:<AB:722++/--,,,,,+,+'()))+-+,.8677:;<;<==<::998;;;;?<?989525323*(()445---,%%%%%$$%''*+))-,-***+)-..*+,*+,,-0/5:?><=<==??;;;741119<=887876<HG<9988><<<C@?@@;;;;<B>=<77764245-,)))0675640((*++,1+,,,299/+,,-:=<<=<<:767+*)')))'&&$#$%(+,+*)0037-+,533334<75100.-//4,,++&&'''570/000631-((-223457778>8:BB@??@??GIG@BEA==<<=A?@B@<744447=,))()3477:76667>;;::>988889430/.)))*/++'&&&&)((&'''*::@999:=@?955669;;;<=@3/-002?AA?<;3((66?CCEEFECEC?>>??ABA??;/)&&*)0(&&&'('&&%%&&&'/00445687:77100/11/(
@934324a6-fb8e-4bac-a35b-0a94b12e66ab runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=13458 ch=471 start_time=2021-06-24T10:08:35Z
CTATGTGCATCTACTTTCATTCCATACATATCTCTGATCTCAGGGTTGACTCTGGTCAAGAGACTTGCATGTGTAATTTTTAGCTAAAGTTGATAGTAAGAATCAGACAAGCCTTTGTGCTTGCGGAGCTTGTTAGGGCCCATCTTAACATCTTCAGTGTTATGCTTTCATTAAGCTACGCTAGCTTTATGCATTATTTTAATAGTGTAAATAGACACATTTTGGGCTTAAATTATACTACTGAGGTTTCCCTGTTGCAGGGGGGTTTTCCAAGTGTAGTCATCTCAGAGAAGTATCGAGATCAGGATTACGCCAAAAACCCCAGGGGTATCTTGAGGTTTTAGGAGTTATATTC

input_1/fastq_runid_6d7597e92539e514bba241b5892ca714de4705c7_3_0.fastq

@ed063915-482d-4b96-a782-f2853ebf409f runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=13226 ch=68 start_time=2021-06-24T10:09:19Z
GTTCTTGGTTCAGTTTCGTATTGCTTACACGACGCTCTTCCGATCTGTCGGCGCTAGTAGCCAGAGCATGGGCTTATTAAACGAATGCCGGAGGTTAAAATCCTCCCTACTGCTCAGAAAAAGGAGACTCTAACTCCTGCCCCTAACTCCCAAAGCTAGGATTCTAACATTAAACTATTCTCTGCCATTTCACAGCGCATATATAATATTTTAGTTTTTTAAAGACATATATGTACTATTACCATATATATATTGTATGACATAACAGTACATGCTATAATAACATATATGTATTATCACCATTAGTTGGAATTAACCACTCAGGTAATACACACCCATATAACAGTACATGGGCCCAAATTTATCGGCCAAAGAACTGAATTTAAATTAAAATATCCAATATGCATCTCCCAGTCCAACCTGCGAAGGTCAATGTTATACCAAGTAACCCTCATCTCGTTCAATTGATCAAAATTAATGTAGTAAAGACCTACCAACCGGTGATAACTAAATGATAACGGTTATTGAAGGTGAGGGACAAAAATAAGTTGGGGGTTAGCTTTAGTGAACTATTCCTGGCATTTGGTTCCTACTTCAGGGCCATTGATTGCTTTATTCCCCGCACTTTCATCGACGCTTGCATAAGTTAATGGTGGCATACATTAGGACTAGTTACCCACCATGCCGAGCGTTCTCTCTCGGGGGTCAGTGGTTCTCTTTTTTTTTCCTTTCCACATCTGGCATCCCAGAGTGCACACGGTATTAATTAAGGTTGAACATTTCCTTGCGCGCGAGGAAATAGTATGAATGATAAAAAGATATTAATAGAAGAATTGCATAACTGATTTCATGAGCATAAATTATGAATATAACTCCTAAAATCTCATCCCTGGGGTTTTTGCGCATAAACCCTGCAATACTCCTGAGATGACTCTAACACTCCTGAAAACCCCCCGGAAACAGGAAAGCCTCTAGAAGTTTTTTTTTATCCCAAAGTGCATGTATATTAATTATTATAATATTTCACATGCTAGCGTAGCTTAATTAAAGCATAACACTGAAGATGTTAAGATGGGCCCTAGAAAGCCCCGCAAGCACAAGAGTTTCATCCTAGAGCTACTGTAAGTTAGCTAGATTTACACACATGCAAGATTCTAACACCATCATGAACTGAGATCGGAAGAGCACACGTCTCGCGATACATTGCATGTTTGTTTTCAGTTAGTATTGTTGAGCGTGTGCTCTTGCAATCTAGCAAAGCGAGAATTTGGATACTTGCATGTGTAAATATAGCTAGAGCTTGATAGTAAAGTCAGGACCAAGCCTTTGTGCTCGCGGGACTTTCTAGGGTCCATCTTAACATCTTCAGTGTTATGCTTTAGATTAAGCTACGTTAGCGTGTGAATTATTATAATGGTGTAAATAAACGCATTTTGGGGTAAAAACACATCTAGAGGTTTTCCTGTTTCCGGGGATTTGCAGGAGTCTTAGTGATCTCAGGAGTGTAAGGGGGTAGGGGGGTTTGACGCAAAAACCGAAGAGGGGGCGATAACGGATAAATAAGAGAAATATCAACATTATGCTCATGATATCCAATTATGCAATTCTTCTGTGATGTCCGGTGTCATTCATAATTATCATTATATTCAAGGAAAATGTTCACCCTTACTTTCAATACCGCCTGCACTGTGAGATGTCAAGTGAAAAGAGAAACGAAAAAAGAGAACCCGTTGCCCGCTGGAGTGAACGCCCGGCATGGTGGGTAACGATTTCTATGGATTAAAAGCATTAACTTATGCAAGCGTCAGATGAAAGTTAGGGAATTATAACAATTCATGGCCCTGAAATAGGAACCAGATGCCAGGAATCATTCACTAAGTGAAACCCCCACGATTTCTGTCCCTGACCATCAATAAACGTATGCATTAAGAAATCAACTGATGGTAGGTTCTTATTACATCGAATGTTTTCGGAATTACGGGTAGTGGATGACTTGGTATATATGGGGTCCTTCGTTTCTTGTTAGATCTTAAATCGCTAACTGTCTTATTAAAGAGTCTTATGTAAGCCAGTAATTATTATGCTTTGTGTAATCCCTTTTATTCAATGGTTACCTGAATGCATTTATAACATAAGATGGTGTTAATACATTAAATGTCTAGTTTACATGTATATATATATGTACTAAAGATATATATGTTACCCTTATGTAATGTCCTACTCATTCATTATATGGGTAATACTTTGTATCTGTAAGATAACCATATATGCACTATAGGAGAATAGTTTTAATTTAGAATACTAGCTTTGGGAGTTAGTGGTGGGTGTTAGAATCACTCTTCTTTGAGCAAAAGGGGGGACTTTAACCTCCGACATCCGGGTTACCGAGCCCGGCGCTTTCTTAAAAAGAGAGACAGATCGGAAGAGCGTCGTGTAAGCAATAC
+
####$%$%'''((&&$$%&*,/..../453,--++,,,--.33344442/...--**+++,21//10..,,*''(%%%$$+(%$$$%)0..11588:?@>>>>==>=<;:::89.,+('*)..5=<<<=<>33))(((-.6<37223954/')3338:::;:8889>DDA3/---//--)(()),,,.23+,1,,-56668@H{HCD?<;93/799AFHKDC?><<:;<?C@DBC??????=;:;=?{{{{{{96677455556;<@??@AB>;:::<=FEDDC@BBBDFDCCCCDDA?<;;<<>=>>A:;;;<>?>A@AA><;:;>>@DDCCF@A>>>?>>>@=<;<>A@=:99:;;;==?B=@8////7888794500011@;<D=>HEEDDCE>>;:;:

input_1/fastq_runid_6d7597e92539e514bba241b5892ca714de4705c7_4_0.fastq

@0544ecf8-46e4-4061-b072-1e8cb5e11f73 runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=12170 ch=146 start_time=2021-06-24T10:10:01Z
ATCTGTCATCTACTTCGTTCAAGATACGTATTGCTGAGCGTGTGCTCTTCCGATCTTAGGGTTGACTCATACAAACTGCATGTGTAAGTTTAGCTAAAGTTGATAGAGTAGGGACCAAGCCTTTGTGCTTGCGGAGCTTTCTAGGGCCCATCTTAACATCTTCAGTGTTATGCTTTAATTAAGCTACGCTAGCTTTATGCATTATTTTAATAGTGTAAATAGACACATTTTGGGCTTAAATTATACTACTAGAGGTTTTCCTGTTTCCGGGGGGTTCAGGAGTGTTAGTCATCTCGACTGGGGAGTAGGGGGGTTTTACGCGTAAAAGCCAAGGGGGAATCTTGGATATGTTAGGGAGAAGTGGTTACACCTTATGCTCTTGATATCCTAATATGTGGTTATTGATTACAATATTTTATCACCATGAATACTATTTAGGCAGCAAAATGTCTTCCTTATTAGTTATATACATAGCTATCTGAAATGTAGCAAAGTGGAAGGAAGACAAAAAAGAACCCCTGACCCCTAGAGAGAACGCTCGGCATGGTGGGTAACAAGTACCTAATGTATGCCAGTAACTTTATGCAAGCGTCGATGAAAGAGTGGGGAATGATATCAATTAATGGCCCTGAAGAAAGAACCAGATGCCAGGAATAATTCACTAAGTGAACACCAGAATAATTGTTCCTCACCATCATTAGGCCGTCATTTTAAAATCAACTGATGGTCGGTTCTTACTACATTAAGATTCTGATATTGACGAGATTTCATCCTGGTATATCTAGACTCATGGATTAGTTTCGCGGTCGGTCTTAAATCTCGCTTGTCCATATTTTCAATTAATGTTAATTACATTTCTAGGTTTGCGTAACCCTTAGTTAAATATGTGTTGGTTATAATGGTTAACTCCTATTAATGTCTTATAAATATTAATGTACTATGATATCATTGATGTGTTTAATATTAATTTAATGGTGTGAATACGTATATGCATTTTTTGAAACAAGAATAGTTTAATTTTAGAATCCTAGCTTTGGGAGTTGGGGGTCAGAGTTAAAATCCCTTCTTTGAGCAGTAGGGAGGATTTTAACCTCCGGCATCCGGCTTACAAGACCGATGCTCTCCCACGGGCAACTGACAGATCGGAAGAGCGTCGTGTAAGCAATAC
+
#$$%%%%'''+*)**'&''*1+**)))(()+,>==****(((,,45)''')))().*'''(-44557'&&&'*)(((*34;;???=>=?BCB@@@@@BC@@525/)&&%%%%%%&)++0112273399:92200135553*,+,/007888>CD@@@87777???>>@A@@>=??@@EEBFMHAA@854448>?@BEFA?>??@@A@;:@BB@@??>?>@A>>>>?A?BC:466566689;<EFDAA:9952223;<?:>@>=>63(((('(()))))64347544../--1-+'$$$$&&)567777:;=B>444447-+++,/0?A;8/+.'&()*++,-,%%%%&*)((%%%%$'()'&'((()49<;;=<=@?@>??==>@DB===>ADEDD@???:::::@AB>?>>>CCB/*A@><;87789@@A?==>=1+***++'%%$&()%%%%&&(773))-.-0+.)'&&&))++)&&&()9976-,,,-.-.0++++,36+++**.0/+()*22/;5...-.<=3/013{88668910144;;;<@65668@(((((((((34?A@@777-''&'(554));:0/////)((()48BCHB><899:876300/+''''--.-/778<2)))//-**,-.211226?>@ACFFIHHFB<<<7,*(('(*''%$$&()()*))*$$%()'((**'&%&%$####%%%&'('%%&&'+*****,)&%%$$$$$&)(%%(''')*..34450//11545523*)))+23'))))*-23CBAG>>>>=76666BC?>C.+***(...09><==<;===BQHH@;;;;=AA@@AA@BF3/DCB?@AABGFFEGFEG>@@5.2?>?BCDA>::221126:,,,,,04<0000066669=?FFGFDCCB=?>>==;*(&$$$$%%&'():71./00'-???>==@@?AABD88878===@:876&*)'*)%$%,+(*0-//++***//80///1{-****++.2/*)'((()*,13466879<75555ACFBCDCABCCBBAA?@BD?@DCC?.-,,,-4&%&'(''(,1&&+'#$%&((())))0A???@CJGGBCA@@>=B@?==4499AA?<-))*:;<FDFHCB>?:56;;<@ADEBABB=====ABCDCDEC;<;;<CCFDCCCDD>5
@90030a16-ee39-4418-a1be-ce066c1aedbf runid=6d7597e92539e514bba241b5892ca714de4705c7 sampleid=Kasai36 read=15506 ch=243 start_time=2021-06-24T10:10:01Z
TCTTGTATTCTACTTGGTTCATTTCATATTGCTGGATTTTGCTCTTCCGATCTCAGCTTACTGCAACCTCCATCTCCTGGGTTCAAGAGATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACACCACCACGTCCCACTGATTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTTAGTCAGGATGGTCTCGATCTCCTGACCTCGTGATCTGCCCACCTCGGCCTCCCACAATGCTGGGATTACAGGCGTGAGCCACCGCACCCGGCCCCTGTATACTAACCTTCCTATGTATTACAAAGGTAGGGTGGACTGGACAGATAGAAACACATCTAGCAAT
+

Option

input_1/

Output

result.txt

Reads	Cluster ID_Number of UMI	Species	Top hit similarity to MitoFISH	Alignment length	Mismatchs	Gap length	Sequence
39	37_1	Gnathostomata;Teleostomi;Euteleostomi;Actinopterygii;Actinopteri;Neopterygii;Teleostei;Osteoglossocephalai;Clupeocephala;Euteleosteomorpha;Neoteleostei;Eurypterygia;Ctenosquamata;Acanthomorphata;Euacanthomorphacea;Percomorphaceae;Carangaria;Carangiformes;Carangidae;Trachurus;Trachurus japonicus	99.349	1076	6	1	ACCGGATGCCGGAGGTTAAAATCCTCCCTACTGCTCAGAAAAAGGAGACTCTAACTCCTGCCCCTAACTCCCAAAGCTAGGATTCTAACATTAAACTATTCTCTGCCATTTCACAGCGCATATATAATATTTTAGTTTTTTAAAGACATATATGTACTATTACCATATATATATTGTATGACATAACACTACATGCTATAATAACATGTATGTATTATCACCATTAGTTGGAATTAACCACTCAGGTTATACACACCCATATAACAGTACATGGGCCCAAATTTATTAACCAAAGAACTGAACTTAAATTAAAATATCCAATATGCATCTCCCAGTCCAACCTGCAAAGGTCAATGTTATACCAAGTAACCCTCATCTCGTTCAATTGATAGAAATTAATGTAGTAAGAGCCTACCAACCGGTGATAACTAAGTGATAACGGTTATTGAAGGTGAGGGACAAAAATCGTGGGGGTTTCACTTAGTGAACTATTCCTGGCATTTGGTTCCTACTTCAGGGCCATTGATTGCTTTATTCCCCGCACTTTCATCGACGCTTACATAAGTTAATGGTGGGAATACATACTCCTCGTTACCCAGCATGCCGGGCGTTCTTTCCAGCGGGTAAGGGGTTCTCTTTTTTGTCTTCCTTTCACTTGACATTTCAGAGTGCGCACGGTATATAACTAATAAGGTAGAACATTTCCTTGCCCGCCGTAAATAGTATTCATGGTGATAAGATATTGTAATCGATAACCACATATTAGGATATCAAGAGCATAAGGTGTAACTACTTCTCCTAACATATCTAAGATTCCCCCCCTTGGCTTTTACGCGTAAAACCCCCCTACCCCCTAAACTCCTAAGATCGCTGTCATTCCTGAAAACCCCCCGGAAACAGGAAAGCCTCTAGAAGTTTTTTTTTATCCCAAAGTGCATGTATATAAATTATTATAATATTTCACATGCTAGCGTAGCTTAATTAAAGCATAACACTGAAGATGTTAAGATGGGCCCTAGAAAGCCCCGCAAGCACAAAGGTTTGGTCCTGACTTTACTGTCAGCTTTAGCTAGAT
43	36_1	Gnathostomata;Teleostomi;Euteleostomi;Actinopterygii;Actinopteri;Neopterygii;Teleostei;Osteoglossocephalai;Clupeocephala;Euteleosteomorpha;Neoteleostei;Eurypterygia;Ctenosquamata;Acanthomorphata;Euacanthomorphacea;Percomorphaceae;Carangaria;Carangiformes;Carangidae;Trachurus;Trachurus japonicus	99.442	1076	6	0	ACCGGATGCCGGAGGTTAAAATCCTCCCTACTGCTCAGAAAAAGGAGACTCTAACTCCTGCCCCTAACTCCCAAAGCTAGGATTCTAACATTAAACTATTCTCTGCCATTTCACAGCGCATATATAATATTTTAGTTTTTTAAAGACATATATGTACTATTACCATATATATATTGTATGACATAACACTACATGCTATAATAACATGTATGTATTATCACCATTAGTTGGAATTAACCACTCAGGTTATACACACCCATATAACAGTACATGGGCCCAAATTTATTAACCAAAGAACTGAACTTAAATTAAAATATCCAATATGCATCTCCCAGTCCAACCTGCAAAGGTCAATGTTATACCAAGTAACCCTCATCTCGTTCAATTGATAGAAATTAATGTAGTAAGAGCCTACCAACCGGTGATAACTAAGTGATAACGGTTATTGAAGGTGAGGGACAAAAATCGTGGGGGTTTCACTTAGTGAACTATTCCTGGCATTTGGTTCCTACTTCAGGGCCATTGATTGCTTTATTCCCCGCACTTTCATCGACGCTTACATAAGTTAATGGTGGGAATACATACTCCTCGTTACCCAGCATGCCGGGCGTTCTTTCCAGCGGGTAAGGGGTTCTCTTTTTTGTCTTCCTTTCACTTGACATTTCAGAGTGCGCACGGTATATAACTAATAAGGTAGAACATTTCCTTGCCCGCCGTAAATAGTATTCATGGTGATAAGATATTGTAATCGATAACCACATATTAGGATATCAAGAGCATAAGGTGTAACTACTTCTCCTAACATATCTAAGATTCCCCCCTCTTGGCTTTTACGCGTAAAACCCCCCTACCCCCTAAACTCCTAAGATCGCTGTCATTCCTGAAAACCCCCCGGAAACAGGAAAGCCTCTAGAAGTTTTTTTTTATCCCAAAGTGCATGTATATAAATTATTATAATATTTCACATGCTAGCGTAGCTTAATTAAAGCATAACACTGAAGATGTTAAGATGGGCCCTAGAAAGCCCCGCAAGCACAAAGGTTTGGTCCTGACTTTACTGTCAGCTTTAGCTAGAT
29	31_1	Gnathostomata;Teleostomi;Euteleostomi;Actinopterygii;Actinopteri;Neopterygii;Teleo

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