assemble~platanus-allele

Platanus-allee is an assembler derived from Platanus assembler, however, it was developed with another concept. Platanus-allee tries to construct each haplotype sequence from the beginning and pair them as homologous chromosomes, while Platanus constructs consensus sequence of homologous chromosomes at first and tries to split into each haplotype sequence. Therefore, Platanus-allee marks better performance for highly heterozygous species genome or highly diverged genomic regions. However, for low heterozygous species genome (as a guide < 1.0 %), Platanus assembler would mark better performance than Platanus-allee. This may be caused by the sequence coverage for the target sequence. Platanus-allee targets each haplotype, therefore, simply speaking, it requires a double number of reads.

input_1:paired-end FASTQ

input_1/kh005_1.fastq.gz

input_1/kh005_2.fastq.gz

input_2:mate-pair FASTQ

input_3:long-read file (PacBio, Nanopore) FASTQ

Option

-c 8 -m 200 input_1/

Output

platanus_contig.fa

>seq1_len229_cov78_read151_maxK125
TATTTTTAATGAACAATAGCAAACTTCATTGCTATTGTCTCGTCACACAATTAAATTATTTATCTTAGCTAATGTCGGTT
TCAACCAACAATGTCTAATTACAAATTAATTGATGATTTAATTGCGTATTAGACCTTAATGCTGCTGGAACGAGATGATT
TGTTCACCGTTGCAAAAACAAAGTATCAGGGATTCTTTGAAAGCAGTCGTGATGCACAACCCAAAGGTC
>seq2_len125_cov117_read151_maxK125
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
>seq3_len177_cov67_read151_maxK125
TGACTCTGCTAGCAGAAACATGCATACAGTGTGAGTCTGGATCTAATATGATTAAAAGTGACTAGTTTGTGTCCAAAAAC
ACTGAAATTGATAGAAACAAATGAAAATGCATATACTGTGTGTGCCGTCACTCTGCTAGCAGAAACATGCATTCGGTGTG

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