-successfully changed directories to /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/annotation~funannotate/test/fun/predict_misc/EVM/scaffold_18/scaffold_18_12403097-12670714

-reading genomic sequence.

** Processing features (+)

-finding all potential splice sites, looking for GT/GC donors and AG acceptors.
-finding all potential starts and stops. (strand: +)
-loading gene prediction evidence. (strand: +)Valid prediction for snap_scaffold_18-snap.2659-T1
Valid prediction for snap_scaffold_18-snap.2686-T1
Valid prediction for GlimmerHMM_glimmerT_43273-T1
Valid prediction for pasa_asmbl_20967.p1
Couldn't classify 19267, 20430
Sorry, prediction pasa_asmbl_20964.p1 fails validation.
(ATG)	 GC	19267-20430	AC	GGA
Valid prediction for Augustus_model.g28821.t1
Valid prediction for Augustus_model.g28800.t1
Valid prediction for GlimmerHMM_glimmerT_43261-T1
Valid prediction for snap_scaffold_18-snap.2660-T1
Valid prediction for Augustus_model.g28819.t1
Valid prediction for GlimmerHMM_glimmerT_43277-T1
Valid prediction for GlimmerHMM_glimmerT_43253-T1
Valid prediction for GlimmerHMM_glimmerT_43275-T1
Valid prediction for GlimmerHMM_glimmerT_43272-T1
Valid prediction for GlimmerHMM_glimmerT_43281-T1
Valid prediction for snap_scaffold_18-snap.2668-T1
Valid prediction for pasa_asmbl_20969.p1
Valid prediction for Augustus_model.g28801.t1
Valid prediction for snap_scaffold_18-snap.2665-T1
Valid prediction for snap_scaffold_18-snap.2680-T1
Valid prediction for GlimmerHMM_glimmerT_43274-T1
Valid prediction for GlimmerHMM_glimmerT_43278-T1
Valid prediction for snap_scaffold_18-snap.2688-T1
Valid prediction for GlimmerHMM_glimmerT_43263-T1
Valid prediction for snap_scaffold_18-snap.2676-T1
Valid prediction for snap_scaffold_18-snap.2655-T1
Valid prediction for HiQ_model.g28818.t1
Valid prediction for snap_scaffold_18-snap.2669-T1
Valid prediction for snap_scaffold_18-snap.2657-T1
Valid prediction for GlimmerHMM_glimmerT_43283-T1
Valid prediction for snap_scaffold_18-snap.2694-T1
Valid prediction for CodingQuarry_transcript_23914-T1
Valid prediction for GlimmerHMM_glimmerT_43276-T1
Valid prediction for GlimmerHMM_glimmerT_43254-T1
Valid prediction for GlimmerHMM_glimmerT_43255-T1
Valid prediction for HiQ_model.g28816.t1
Valid prediction for GlimmerHMM_glimmerT_43262-T1
Valid prediction for snap_scaffold_18-snap.2684-T1
Valid prediction for Augustus_model.g28810.t1
Valid prediction for Augustus_model.g28811.t1
Valid prediction for GlimmerHMM_glimmerT_43286-T1
Valid prediction for GlimmerHMM_glimmerT_43265-T1
Valid prediction for GlimmerHMM_glimmerT_43259-T1
Valid prediction for Augustus_model.g28805.t1
Valid prediction for snap_scaffold_18-snap.2664-T1
Valid prediction for GlimmerHMM_glimmerT_43269-T1
Valid prediction for snap_scaffold_18-snap.2695-T1
Valid prediction for snap_scaffold_18-snap.2690-T1
Valid prediction for GlimmerHMM_glimmerT_43256-T1
Valid prediction for Augustus_model.g28802.t1
Valid prediction for snap_scaffold_18-snap.2677-T1
Valid prediction for snap_scaffold_18-snap.2673-T1
Valid prediction for snap_scaffold_18-snap.2652-T1
Valid prediction for Augustus_model.g28814.t1
Valid prediction for Augustus_model.g28820.t1
Valid prediction for snap_scaffold_18-snap.2662-T1
Valid prediction for GlimmerHMM_glimmerT_43285-T1
Valid prediction for snap_scaffold_18-snap.2683-T1
Valid prediction for GlimmerHMM_glimmerT_43279-T1
Valid prediction for Augustus_model.g28812.t1
Valid prediction for Augustus_model.g28809.t1
Valid prediction for GlimmerHMM_glimmerT_43284-T1
Couldn't classify 208621, 208742
Sorry, prediction pasa_asmbl_20970.p1 fails validation.
(ATG)	 AC	207646-207727	GT	GGG
(GTT)	 AG	207874-208039	GT	AGG
(CAA)	 AG	208359-208465	GT	GGG
(GCG)	 AG	208621-208742	CG	GAC
-recovered pasa_asmbl_20970.p1, initial, 207646, 207727
-recovered pasa_asmbl_20970.p1, internal, 207874, 208039
-recovered pasa_asmbl_20970.p1, internal, 208359, 208465
Couldn't classify 210075, 210104
Sorry, prediction pasa_asmbl_20971.p1 fails validation.
(GGC)	 CA	210075-210104	GT	AGG
(AGT)	 AG	210409-210462	GT	AGG
(TAT)	 AG	210887-210953	GT	AGG
(GCT)	 AG	211309-211409	GT	TGG
(GGT)	 AG	211606-211750	GT	GAG
(TGC)	 AG	212591-212673	GT	AGG
(TTC)	 AG	214315-214464	AC	GAA
add_exon() Sorry, pasa_asmbl_20971.p1 211606-211750-terminal-3 is invalid.
-recovered pasa_asmbl_20971.p1, terminal, 211606, 211750
-recovered pasa_asmbl_20971.p1, terminal, 214315, 214464
-recovered pasa_asmbl_20971.p1, internal, 210409, 210462
-recovered pasa_asmbl_20971.p1, internal, 210887, 210953
-recovered pasa_asmbl_20971.p1, internal, 211309, 211409
-recovered pasa_asmbl_20971.p1, internal, 211606, 211750
-recovered pasa_asmbl_20971.p1, internal, 212591, 212673
Couldn't classify 37184, 39190
Sorry, prediction pasa_asmbl_20965.p1 fails validation.
(GGC)	 CA	37184-39190	GG	AGG

-loading the search evidence (protein and EST search results, strand: +)
-scoring exons (strand: +)

** Processing features (-)

-finding all potential splice sites, looking for GT/GC donors and AG acceptors.
-finding all potential starts and stops. (strand: -)
-loading gene prediction evidence. (strand: -)Valid prediction for snap_scaffold_18-snap.2658-T1
Valid prediction for pasa_asmbl_20156.p1
Valid prediction for GlimmerHMM_glimmerT_43268-T1
Valid prediction for snap_scaffold_18-snap.2701-T1
Valid prediction for GlimmerHMM_glimmerT_43282-T1
Valid prediction for snap_scaffold_18-snap.2666-T1
Valid prediction for snap_scaffold_18-snap.2675-T1
Valid prediction for pasa_asmbl_20150.p1
Valid prediction for HiQ_model.g28806.t1
Valid prediction for snap_scaffold_18-snap.2674-T1
Valid prediction for snap_scaffold_18-snap.2702-T1
Valid prediction for snap_scaffold_18-snap.2663-T1
Valid prediction for GlimmerHMM_glimmerT_43270-T1
Valid prediction for snap_scaffold_18-snap.2685-T1
Couldn't classify 171836, 171907
Sorry, prediction pasa_asmbl_20152.p1 fails validation.
(ACC)	 AG	171836-171907	GT	AGG
(CTG)	 AG	172035-172123	GT	TGG
(GGA)	 AG	172327-172402	GT	TGG
(GAT)	 AG	172817-172930	GT	AGG
(GCC)	 AG	173046-173136	GT	TGG
(GTG)	 AG	175035-175114	GT	AGG
(GAA)	 AG	176598-176657	GT	GGG
(CTG)	 AG	176741-176886	GT	AAG
(CTG)	 AG	177050-177098	GT	AGG
(AGG)	 AG	177515-177584	GT	TGG
(GCC)	 AG	177709-177824	GT	AGG
(GAG)	 AG	178306-178495	GT	TGG
(GTC)	 AG	178885-178997	GT	CGG
(GAG)	 AG	179438-179540	GT	GGG
(CTT)	 AG	179770-179930	GT	TGG
(GAG)	 AG	180126-180251	GT	AGG
(ATG)	 AG	180436-180549	GT	AGG
(GAC)	 AG	181034-181201	GT	AGG
(GTG)	 AG	181450-181557	AG	GAA
-recovered pasa_asmbl_20152.p1, initial, 180436, 180549
-recovered pasa_asmbl_20152.p1, terminal, 181450, 181557
-recovered pasa_asmbl_20152.p1, internal, 171836, 171907
-recovered pasa_asmbl_20152.p1, internal, 172035, 172123
-recovered pasa_asmbl_20152.p1, internal, 172327, 172402
-recovered pasa_asmbl_20152.p1, internal, 172817, 172930
-recovered pasa_asmbl_20152.p1, internal, 173046, 173136
-recovered pasa_asmbl_20152.p1, internal, 175035, 175114
-recovered pasa_asmbl_20152.p1, internal, 176598, 176657
-recovered pasa_asmbl_20152.p1, internal, 176741, 176886
-recovered pasa_asmbl_20152.p1, internal, 177050, 177098
-recovered pasa_asmbl_20152.p1, internal, 177515, 177584
-recovered pasa_asmbl_20152.p1, internal, 177709, 177824
-recovered pasa_asmbl_20152.p1, internal, 178306, 178495
-recovered pasa_asmbl_20152.p1, internal, 178885, 178997
-recovered pasa_asmbl_20152.p1, internal, 179438, 179540
-recovered pasa_asmbl_20152.p1, internal, 179770, 179930
-recovered pasa_asmbl_20152.p1, internal, 180126, 180251
-recovered pasa_asmbl_20152.p1, internal, 180436, 180549
-recovered pasa_asmbl_20152.p1, internal, 181034, 181201
Valid prediction for snap_scaffold_18-snap.2672-T1
Couldn't classify 170521, 170649
Sorry, prediction pasa_asmbl_20153.p1 fails validation.
(ATG)	 CA	167752-167759	GT	TGG
(GGA)	 AG	168174-168256	GT	CTG
(TTC)	 AG	168480-168572	GT	ACG
(TCA)	 AG	169042-169154	GT	TGG
(AAC)	 AG	169401-169530	GT	TGG
(CCT)	 AG	170061-170164	GT	AGG
(GTG)	 AG	170521-170649	TG	GGT
-recovered pasa_asmbl_20153.p1, initial, 167752, 167759
-recovered pasa_asmbl_20153.p1, internal, 168174, 168256
-recovered pasa_asmbl_20153.p1, internal, 168480, 168572
-recovered pasa_asmbl_20153.p1, internal, 169042, 169154
-recovered pasa_asmbl_20153.p1, internal, 169401, 169530
-recovered pasa_asmbl_20153.p1, internal, 170061, 170164
Valid prediction for Augustus_model.g28804.t1
Couldn't classify 63135, 63268
Couldn't classify 64540, 64586
Sorry, prediction pasa_asmbl_20232.p1 fails validation.
(CAG)	 TG	63135-63268	GT	AGG
(TCT)	 AG	63966-64114	GT	AGG
(CCT)	 AG	64540-64586	GA	TGG
-recovered pasa_asmbl_20232.p1, internal, 63966, 64114
Valid prediction for GlimmerHMM_glimmerT_43264-T1
Valid prediction for snap_scaffold_18-snap.2687-T1
Valid prediction for snap_scaffold_18-snap.2653-T1
Valid prediction for snap_scaffold_18-snap.2682-T1
Valid prediction for snap_scaffold_18-snap.2691-T1
Valid prediction for HiQ_model.g28807.t1
Valid prediction for HiQ_model.g28803.t1
Valid prediction for snap_scaffold_18-snap.2681-T1
Valid prediction for snap_scaffold_18-snap.2661-T1
Valid prediction for GlimmerHMM_glimmerT_43280-T1
Valid prediction for Augustus_model.g28808.t1
Valid prediction for snap_scaffold_18-snap.2689-T1
Valid prediction for CodingQuarry_transcript_23912-T1
Valid prediction for snap_scaffold_18-snap.2696-T1
Valid prediction for GlimmerHMM_glimmerT_43252-T1
Valid prediction for GlimmerHMM_glimmerT_43271-T1
Valid prediction for GlimmerHMM_glimmerT_43266-T1
Valid prediction for snap_scaffold_18-snap.2678-T1
Valid prediction for snap_scaffold_18-snap.2693-T1
Valid prediction for pasa_asmbl_20158.p1
Valid prediction for snap_scaffold_18-snap.2700-T1
Valid prediction for snap_scaffold_18-snap.2667-T1
Valid prediction for Augustus_model.g28817.t1
Valid prediction for snap_scaffold_18-snap.2703-T1
Valid prediction for GlimmerHMM_glimmerT_43267-T1
Valid prediction for HiQ_model.g28815.t1
Valid prediction for pasa_asmbl_20151.p1
Valid prediction for snap_scaffold_18-snap.2679-T1
Valid prediction for GlimmerHMM_glimmerT_43260-T1
Couldn't classify 60174, 60226
Couldn't classify 61847, 61879
Sorry, prediction pasa_asmbl_20160.p1 fails validation.
(CAC)	 AA	60174-60226	GT	AAG
(CAT)	 AG	60875-61044	GT	CGG
(GCT)	 AG	61145-61278	GT	AGG
(GTG)	 AG	61847-61879	TC	GAT
-recovered pasa_asmbl_20160.p1, internal, 60875, 61044
-recovered pasa_asmbl_20160.p1, internal, 61145, 61278
Valid prediction for snap_scaffold_18-snap.2697-T1
Valid prediction for GlimmerHMM_glimmerT_43287-T1
Valid prediction for CodingQuarry_transcript_23913-T1
Valid prediction for snap_scaffold_18-snap.2692-T1
Valid prediction for snap_scaffold_18-snap.2654-T1
Valid prediction for snap_scaffold_18-snap.2671-T1
Valid prediction for Augustus_model.g28813.t1
Valid prediction for GlimmerHMM_glimmerT_43257-T1
Valid prediction for GlimmerHMM_glimmerT_43258-T1
Valid prediction for snap_scaffold_18-snap.2699-T1
Valid prediction for snap_scaffold_18-snap.2670-T1
Valid prediction for snap_scaffold_18-snap.2656-T1

-loading the search evidence (protein and EST search results, strand: -)
-scoring exons (strand: -)Error with prediction: snap lend_intergenic: 20162, rend_intergenic: 19267  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 22567, rend_intergenic: 21950  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 26691, rend_intergenic: 23492  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 29619, rend_intergenic: 29521  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 49681, rend_intergenic: 41167  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 55049, rend_intergenic: 50822  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 64392, rend_intergenic: 60615  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 71605, rend_intergenic: 67639  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 84054, rend_intergenic: 72577  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 96439, rend_intergenic: 86449  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 99031, rend_intergenic: 96670  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 103597, rend_intergenic: 100932  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 133188, rend_intergenic: 105486  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 143896, rend_intergenic: 134926  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 155469, rend_intergenic: 144545  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 163323, rend_intergenic: 161427  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 174036, rend_intergenic: 167654  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 179787, rend_intergenic: 174787  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 188538, rend_intergenic: 185840  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 195630, rend_intergenic: 188726  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 208882, rend_intergenic: 202513  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 214464, rend_intergenic: 211007  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.

-building DP trellis

-building trellis in range 1 - 267618
-exon list size: 534


building trellis

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72.6    
72.8    
72.9    
73.1    
73.3    
73.5    
73.7    
73.9    
74.1    
74.3    
74.4    
74.6    
74.8    
75.0    
75.2    
75.4    
75.6    
75.7    
75.9    
76.1    
76.3    
76.5    
76.7    
76.9    
77.1    
77.2    
77.4    
77.6    
77.8    
78.0    
78.2    
78.4    
78.5    
78.7    
78.9    
79.1    
79.3    
79.5    
79.7    
79.9    
80.0    
80.2    
80.4    
80.6    
80.8    
81.0    
81.2    
81.3    
81.5    
81.7    
81.9    
82.1    
82.3    
82.5    
82.6    
82.8    
83.0    
83.2    
83.4    
83.6    
83.8    
84.0    
84.1    
84.3    
84.5    
84.7    
84.9    
85.1    
85.3    
85.4    
85.6    
85.8    
86.0    
86.2    
86.4    
86.6    
86.8    
86.9    
87.1    
87.3    
87.5    
87.7    
87.9    
88.1    
88.2    
88.4    
88.6    
88.8    
89.0    
89.2    
89.4    
89.6    
89.7    
89.9    
90.1    
90.3    
90.5    
90.7    
90.9    
91.0    
91.2    
91.4    
91.6    
91.8    
92.0    
92.2    
92.4    
92.5    
92.7    
92.9    
93.1    
93.3    
93.5    
93.7    
93.8    
94.0    
94.2    
94.4    
94.6    
94.8    
95.0    
95.1    
95.3    
95.5    
95.7    
95.9    
96.1    
96.3    
96.5    
96.6    
96.8    
97.0    
97.2    
97.4    
97.6    
97.8    
97.9    
98.1    
98.3    
98.5    
98.7    
98.9    
99.1    
99.3    
99.4    
99.6    
99.8    
-found 32 predictions from path traversal
Error with prediction: snap lend_intergenic: 20162, rend_intergenic: 19267  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 22567, rend_intergenic: 21950  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 26691, rend_intergenic: 23492  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 29619, rend_intergenic: 29521  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 49681, rend_intergenic: 41167  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 55049, rend_intergenic: 50822  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 64392, rend_intergenic: 60615  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 71605, rend_intergenic: 67639  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 84054, rend_intergenic: 72577  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 96439, rend_intergenic: 86449  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 99031, rend_intergenic: 96670  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 103597, rend_intergenic: 100932  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 133188, rend_intergenic: 105486  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 143896, rend_intergenic: 134926  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 155469, rend_intergenic: 144545  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 163323, rend_intergenic: 161427  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 174036, rend_intergenic: 167654  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 179787, rend_intergenic: 174787  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 188538, rend_intergenic: 185840  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 195630, rend_intergenic: 188726  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 208882, rend_intergenic: 202513  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 214464, rend_intergenic: 211007  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.

-reporting highest scoring path thru the trellis.
-found 23 predictions persist after filtering for low support

-building DP trellis

-building trellis in range 1 - 15922
-exon list size: 11


building trellis

7.7    
15.4    
23.1    
30.8    
38.5    
46.2    
53.8    
61.5    
69.2    
76.9    
84.6    
92.3    
-found 2 predictions from path traversal
GOT 5prime partial gene.

-reporting highest scoring path thru the trellis.
-found 0 predictions persist after filtering for low support

-building DP trellis

-building trellis in range 1 - 13292
-exon list size: 7


building trellis

11.1    
22.2    
33.3    
44.4    
55.6    
66.7    
77.8    
88.9    
-found 2 predictions from path traversal
GOT 5prime partial gene.
GOT 5prime partial gene.

-reporting highest scoring path thru the trellis.
-found 0 predictions persist after filtering for low support