-successfully changed directories to /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/annotation~Funannotate/fun/predict_misc/EVM/scaffold_23/scaffold_23_2588545-2775931

-reading genomic sequence.

** Processing features (+)

-finding all potential splice sites, looking for GT/GC donors and AG acceptors.
-finding all potential starts and stops. (strand: +)
-loading gene prediction evidence. (strand: +)Valid prediction for HiQ_model.g34864.t1
Couldn't classify 45222, 45452
Sorry, prediction pasa_asmbl_30714.p1 fails validation.
(GCA)	 CG	45222-45452	GT	AGG
(AGG)	 AG	46251-46376	GT	AGG
(CTC)	 AG	46587-46647	GT	ATG
(TCA)	 AG	46826-46941	GC	GAG
-recovered pasa_asmbl_30714.p1, terminal, 46826, 46941
-recovered pasa_asmbl_30714.p1, internal, 46251, 46376
-recovered pasa_asmbl_30714.p1, internal, 46587, 46647
-recovered pasa_asmbl_30714.p1, internal, 46826, 46941
Valid prediction for snap_scaffold_23-snap.504-T1
Valid prediction for GlimmerHMM_glimmerT_51881-T1
Valid prediction for GeneMark_model.90147_t
Valid prediction for GlimmerHMM_glimmerT_51899-T1
Valid prediction for snap_scaffold_23-snap.507-T1
Valid prediction for GeneMark_model.90151_t
Valid prediction for snap_scaffold_23-snap.493-T1
Valid prediction for GlimmerHMM_glimmerT_51879-T1
Valid prediction for snap_scaffold_23-snap.513-T1
Valid prediction for Augustus_model.g34882.t1
Valid prediction for GeneMark_model.90138_t
Valid prediction for GeneMark_model.90152_t
Valid prediction for GeneMark_model.90163_t
Valid prediction for GeneMark_model.90154_t
Valid prediction for Augustus_model.g34865.t1
Valid prediction for snap_scaffold_23-snap.499-T1
Valid prediction for snap_scaffold_23-snap.496-T1
Valid prediction for GlimmerHMM_glimmerT_51883-T1
Valid prediction for GlimmerHMM_glimmerT_51893-T1
Valid prediction for GeneMark_model.90161_t
Valid prediction for snap_scaffold_23-snap.511-T1
Couldn't classify 35126, 35188
Sorry, prediction pasa_asmbl_30711.p1 fails validation.
(ATG)	 GA	33710-33829	GT	GGG
(ATG)	 AG	34018-34156	GT	GGG
(GTC)	 AG	34301-34349	GT	AGG
(GAG)	 AG	34467-34565	GT	AGG
(AGC)	 AG	34684-34744	GT	TGG
(CTC)	 AG	34956-35015	GT	TGG
(CTG)	 AG	35126-35188	TA	AGT
-recovered pasa_asmbl_30711.p1, initial, 33710, 33829
-recovered pasa_asmbl_30711.p1, initial, 34018, 34156
-recovered pasa_asmbl_30711.p1, internal, 34018, 34156
-recovered pasa_asmbl_30711.p1, internal, 34301, 34349
-recovered pasa_asmbl_30711.p1, internal, 34467, 34565
-recovered pasa_asmbl_30711.p1, internal, 34684, 34744
-recovered pasa_asmbl_30711.p1, internal, 34956, 35015
Valid prediction for HiQ_model.g34876.t1
Valid prediction for GeneMark_model.90157_t
Valid prediction for snap_scaffold_23-snap.489-T1
Valid prediction for HiQ_model.g34862.t1
Valid prediction for GeneMark_model.90144_t
Valid prediction for CodingQuarry_transcript_35529-T1
Valid prediction for Augustus_model.g34877.t1
Valid prediction for snap_scaffold_23-snap.508-T1
Valid prediction for GeneMark_model.90160_t
Valid prediction for snap_scaffold_23-snap.506-T1
Valid prediction for snap_scaffold_23-snap.500-T1
Valid prediction for CodingQuarry_transcript_35530-T1
Couldn't classify 55071, 55377
Sorry, prediction pasa_asmbl_30718.p1 fails validation.
(CCT)	 CA	55071-55377	GT	AGG
(CCG)	 AG	55912-56022	GT	CGG
(GGT)	 AG	56199-56351	GT	AAG
(AAT)	 AG	56489-56620	GT	TGG
(TTT)	 AG	56776-56913	GT	AGG
(CTT)	 AG	57014-57139	GT	GGG
(ACG)	 AG	57231-57376	GT	ATG
(CCG)	 AG	57462-57606	GT	AGG
(TGC)	 AG	57774-57932	GT	AGG
(TTA)	 AG	58375-58509	GT	CGG
(ATC)	 AG	58743-59030	GT	TGG
(AGT)	 AG	59169-59266	GT	AGG
(GTG)	 AG	59379-59637	GT	TCG
(GAC)	 AG	59906-60066	GT	TAG
(AGA)	 AG	60147-60357	GT	AGG
(AGT)	 AG	60569-60679	GT	AGG
(GCT)	 AG	60825-61015	GT	AGG
(TTC)	 AG	61119-61232	GT	TGG
(GGC)	 AG	61394-61464	GT	GTG
(GTG)	 AG	61590-61630	GT	AGG
(GAG)	 AG	61744-61840	GT	GTG
(GTA)	 AG	62356-62455	GC	AGG
(ATG)	 AG	62548-62622	TC	GAT
-recovered pasa_asmbl_30718.p1, terminal, 61590, 61630
-recovered pasa_asmbl_30718.p1, terminal, 62548, 62622
-recovered pasa_asmbl_30718.p1, internal, 55912, 56022
-recovered pasa_asmbl_30718.p1, internal, 56199, 56351
-recovered pasa_asmbl_30718.p1, internal, 56489, 56620
-recovered pasa_asmbl_30718.p1, internal, 56776, 56913
-recovered pasa_asmbl_30718.p1, internal, 57014, 57139
-recovered pasa_asmbl_30718.p1, internal, 57231, 57376
-recovered pasa_asmbl_30718.p1, internal, 57462, 57606
-recovered pasa_asmbl_30718.p1, internal, 57774, 57932
-recovered pasa_asmbl_30718.p1, internal, 58375, 58509
-recovered pasa_asmbl_30718.p1, internal, 58743, 59030
-recovered pasa_asmbl_30718.p1, internal, 59169, 59266
-recovered pasa_asmbl_30718.p1, internal, 59379, 59637
-recovered pasa_asmbl_30718.p1, internal, 59906, 60066
-recovered pasa_asmbl_30718.p1, internal, 60147, 60357
-recovered pasa_asmbl_30718.p1, internal, 60569, 60679
-recovered pasa_asmbl_30718.p1, internal, 60825, 61015
-recovered pasa_asmbl_30718.p1, internal, 61119, 61232
-recovered pasa_asmbl_30718.p1, internal, 61394, 61464
-recovered pasa_asmbl_30718.p1, internal, 61590, 61630
-recovered pasa_asmbl_30718.p1, internal, 61744, 61840
-recovered pasa_asmbl_30718.p1, internal, 62356, 62455
Valid prediction for snap_scaffold_23-snap.488-T1
Valid prediction for Augustus_model.g34868.t1
Valid prediction for GlimmerHMM_glimmerT_51889-T1
Valid prediction for GlimmerHMM_glimmerT_51901-T1
Valid prediction for GeneMark_model.90133_t
Valid prediction for snap_scaffold_23-snap.487-T1
Valid prediction for GeneMark_model.90155_t
Valid prediction for snap_scaffold_23-snap.512-T1
Valid prediction for GlimmerHMM_glimmerT_51898-T1
Valid prediction for GlimmerHMM_glimmerT_51895-T1
Valid prediction for snap_scaffold_23-snap.501-T1
Sorry, intron length (9) is less than minimum required (10)
Valid prediction for GeneMark_model.90131_t
Valid prediction for pasa_asmbl_30712.p1
Valid prediction for GeneMark_model.90135_t
Valid prediction for GlimmerHMM_glimmerT_51892-T1
Valid prediction for GeneMark_model.90149_t
Valid prediction for GeneMark_model.90140_t
Valid prediction for Augustus_model.g34873.t1
Valid prediction for GeneMark_model.90134_t
Valid prediction for GlimmerHMM_glimmerT_51890-T1
Valid prediction for snap_scaffold_23-snap.494-T1
Valid prediction for pasa_asmbl_30724.p1
Valid prediction for snap_scaffold_23-snap.491-T1

-loading the search evidence (protein and EST search results, strand: +)
-scoring exons (strand: +)

** Processing features (-)

-finding all potential splice sites, looking for GT/GC donors and AG acceptors.
-finding all potential starts and stops. (strand: -)
-loading gene prediction evidence. (strand: -)Valid prediction for HiQ_model.g34875.t1
Valid prediction for GeneMark_model.90158_t
Valid prediction for GeneMark_model.90139_t
Valid prediction for snap_scaffold_23-snap.482-T1
Valid prediction for GlimmerHMM_glimmerT_51894-T1
Valid prediction for Augustus_model.g34874.t1
Valid prediction for GlimmerHMM_glimmerT_51885-T1
Valid prediction for Augustus_model.g34880.t1
Valid prediction for Augustus_model.g34860.t1
Valid prediction for snap_scaffold_23-snap.514-T1
Valid prediction for CodingQuarry_transcript_35526-T1
Valid prediction for Augustus_model.g34878.t1
Valid prediction for snap_scaffold_23-snap.483-T1
Valid prediction for CodingQuarry_transcript_35527-T1
Valid prediction for snap_scaffold_23-snap.495-T1
Valid prediction for HiQ_model.g34861.t1
Valid prediction for snap_scaffold_23-snap.486-T1
Couldn't classify 182456, 182601
Sorry, prediction pasa_asmbl_31970.p1 fails validation.
(CCA)	 TC	182456-182601	GT	TCG
(GTA)	 AG	182778-183060	TG	GAT
-recovered pasa_asmbl_31970.p1, terminal, 182778, 183060
Valid prediction for GeneMark_model.90153_t
Valid prediction for GeneMark_model.90130_t
Valid prediction for GlimmerHMM_glimmerT_51886-T1
Valid prediction for GeneMark_model.90136_t
Valid prediction for GeneMark_model.90127_t
Valid prediction for GlimmerHMM_glimmerT_51878-T1
Valid prediction for GeneMark_model.90137_t
Valid prediction for GeneMark_model.90146_t
Valid prediction for GeneMark_model.90129_t
Valid prediction for Augustus_model.g34879.t1
Valid prediction for GeneMark_model.90159_t
Valid prediction for GlimmerHMM_glimmerT_51888-T1
Valid prediction for GeneMark_model.90132_t
Valid prediction for GeneMark_model.90143_t
Valid prediction for snap_scaffold_23-snap.490-T1
Valid prediction for GlimmerHMM_glimmerT_51903-T1
Valid prediction for Augustus_model.g34867.t1
Valid prediction for HiQ_model.g34870.t1
Valid prediction for GlimmerHMM_glimmerT_51891-T1
Valid prediction for GeneMark_model.90148_t
Valid prediction for pasa_asmbl_31481.p1
Valid prediction for GlimmerHMM_glimmerT_51875-T1
Couldn't classify 92463, 93212
Sorry, prediction pasa_asmbl_31482.p1 fails validation.
(GAG)	 TT	92463-93212	GC	GAG
Valid prediction for snap_scaffold_23-snap.503-T1
Valid prediction for GeneMark_model.90150_t
Valid prediction for pasa_asmbl_31483.p1
Valid prediction for GlimmerHMM_glimmerT_51900-T1
Valid prediction for GlimmerHMM_glimmerT_51884-T1
Valid prediction for GlimmerHMM_glimmerT_51880-T1
Valid prediction for GlimmerHMM_glimmerT_51882-T1
Valid prediction for snap_scaffold_23-snap.502-T1
Couldn't classify 169657, 169806
Sorry, prediction pasa_asmbl_31467.p1 fails validation.
(TTC)	 TG	169657-169806	GT	ACG
(CCT)	 AG	169909-170186	GT	AGG
(GCC)	 AG	170287-170419	GT	AGG
(ATG)	 AG	170538-170667	GT	AGG
(GCT)	 AG	171006-171139	GT	AGG
(GTT)	 AG	171332-171456	GT	AAG
(GTA)	 AG	171616-171829	GC	GAG
-recovered pasa_asmbl_31467.p1, initial, 170538, 170667
add_exon() Sorry, pasa_asmbl_31467.p1 170538-170667-terminal-3 is invalid.
-recovered pasa_asmbl_31467.p1, terminal, 170538, 170667
-recovered pasa_asmbl_31467.p1, terminal, 171616, 171829
-recovered pasa_asmbl_31467.p1, internal, 169909, 170186
-recovered pasa_asmbl_31467.p1, internal, 170287, 170419
-recovered pasa_asmbl_31467.p1, internal, 170538, 170667
-recovered pasa_asmbl_31467.p1, internal, 171006, 171139
-recovered pasa_asmbl_31467.p1, internal, 171332, 171456
-recovered pasa_asmbl_31467.p1, internal, 171616, 171829
Valid prediction for GeneMark_model.90145_t
Valid prediction for snap_scaffold_23-snap.498-T1
Valid prediction for snap_scaffold_23-snap.509-T1
Valid prediction for Augustus_model.g34871.t1
Valid prediction for GlimmerHMM_glimmerT_51896-T1
Valid prediction for snap_scaffold_23-snap.484-T1
Valid prediction for snap_scaffold_23-snap.497-T1
Valid prediction for snap_scaffold_23-snap.516-T1
Valid prediction for GlimmerHMM_glimmerT_51877-T1
Valid prediction for pasa_asmbl_31469.p1
Valid prediction for Augustus_model.g34866.t1
Valid prediction for CodingQuarry_transcript_35525-T1
Valid prediction for GlimmerHMM_glimmerT_51876-T1
Valid prediction for snap_scaffold_23-snap.505-T1
Valid prediction for GeneMark_model.90128_t
Valid prediction for GlimmerHMM_glimmerT_51887-T1
Valid prediction for GlimmerHMM_glimmerT_51897-T1
Valid prediction for HiQ_model.g34863.t1
Valid prediction for snap_scaffold_23-snap.485-T1
Valid prediction for Augustus_model.g34872.t1
Valid prediction for GlimmerHMM_glimmerT_51902-T1
Valid prediction for snap_scaffold_23-snap.510-T1
Valid prediction for Augustus_model.g34881.t1
Couldn't classify 179555, 179744
Sorry, prediction pasa_asmbl_31966.p1 fails validation.
(ATG)	 CA	178516-178650	GT	CAG
(GAA)	 AG	178749-178880	GT	AGG
(GTG)	 AG	178967-179054	GT	GGG
(GAC)	 AG	179159-179378	GT	TCG
(GTA)	 AG	179555-179744	AT	TAA
-recovered pasa_asmbl_31966.p1, initial, 178516, 178650
-recovered pasa_asmbl_31966.p1, internal, 178749, 178880
-recovered pasa_asmbl_31966.p1, internal, 178967, 179054
-recovered pasa_asmbl_31966.p1, internal, 179159, 179378
Couldn't classify 150498, 150684
Sorry, prediction pasa_asmbl_31479.p1 fails validation.
(CCG)	 CT	150498-150684	GT	AAG
(GCA)	 AG	150862-151044	GT	AGG
(ACC)	 AG	151286-151659	TG	GAT
-recovered pasa_asmbl_31479.p1, terminal, 151286, 151659
-recovered pasa_asmbl_31479.p1, internal, 150862, 151044
Valid prediction for snap_scaffold_23-snap.515-T1
Valid prediction for GeneMark_model.90156_t
Couldn't classify 174843, 174854
Couldn't classify 175769, 176021
Sorry, prediction pasa_asmbl_31466.p1 fails validation.
(CCG)	 AG	174843-174854	GT	CAG
(GAA)	 AG	174955-175086	GT	AGG
(GTA)	 AG	175172-175259	GT	AGG
(GCC)	 AG	175373-175592	GT	TCG
(GTA)	 AG	175769-176021	AA	GGA
-recovered pasa_asmbl_31466.p1, terminal, 175172, 175259
-recovered pasa_asmbl_31466.p1, internal, 174843, 174854
-recovered pasa_asmbl_31466.p1, internal, 174955, 175086
-recovered pasa_asmbl_31466.p1, internal, 175172, 175259
-recovered pasa_asmbl_31466.p1, internal, 175373, 175592
Valid prediction for Augustus_model.g34869.t1
Valid prediction for snap_scaffold_23-snap.492-T1
Valid prediction for GeneMark_model.90162_t

-loading the search evidence (protein and EST search results, strand: -)
-scoring exons (strand: -)Error with prediction: GlimmerHMM lend_intergenic: 181480, rend_intergenic: 181477  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 30792, rend_intergenic: 25084  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 76420, rend_intergenic: 72809  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 145303, rend_intergenic: 142671  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.

-building DP trellis

-building trellis in range 1 - 187387
-exon list size: 536


building trellis

0.2    
0.4    
0.6    
0.7    
0.9    
1.1    
1.3    
1.5    
1.7    
1.9    
2.0    
2.2    
2.4    
2.6    
2.8    
3.0    
3.2    
3.3    
3.5    
3.7    
3.9    
4.1    
4.3    
4.5    
4.6    
4.8    
5.0    
5.2    
5.4    
5.6    
5.8    
5.9    
6.1    
6.3    
6.5    
6.7    
6.9    
7.1    
7.2    
7.4    
7.6    
7.8    
8.0    
8.2    
8.4    
8.6    
8.7    
8.9    
9.1    
9.3    
9.5    
9.7    
9.9    
10.0    
10.2    
10.4    
10.6    
10.8    
11.0    
11.2    
11.3    
11.5    
11.7    
11.9    
12.1    
12.3    
12.5    
12.6    
12.8    
13.0    
13.2    
13.4    
13.6    
13.8    
13.9    
14.1    
14.3    
14.5    
14.7    
14.9    
15.1    
15.2    
15.4    
15.6    
15.8    
16.0    
16.2    
16.4    
16.5    
16.7    
16.9    
17.1    
17.3    
17.5    
17.7    
17.8    
18.0    
18.2    
18.4    
18.6    
18.8    
19.0    
19.1    
19.3    
19.5    
19.7    
19.9    
20.1    
20.3    
20.4    
20.6    
20.8    
21.0    
21.2    
21.4    
21.6    
21.7    
21.9    
22.1    
22.3    
22.5    
22.7    
22.9    
23.0    
23.2    
23.4    
23.6    
23.8    
24.0    
24.2    
24.3    
24.5    
24.7    
24.9    
25.1    
25.3    
25.5    
25.7    
25.8    
26.0    
26.2    
26.4    
26.6    
26.8    
27.0    
27.1    
27.3    
27.5    
27.7    
27.9    
28.1    
28.3    
28.4    
28.6    
28.8    
29.0    
29.2    
29.4    
29.6    
29.7    
29.9    
30.1    
30.3    
30.5    
30.7    
30.9    
31.0    
31.2    
31.4    
31.6    
31.8    
32.0    
32.2    
32.3    
32.5    
32.7    
32.9    
33.1    
33.3    
33.5    
33.6    
33.8    
34.0    
34.2    
34.4    
34.6    
34.8    
34.9    
35.1    
35.3    
35.5    
35.7    
35.9    
36.1    
36.2    
36.4    
36.6    
36.8    
37.0    
37.2    
37.4    
37.5    
37.7    
37.9    
38.1    
38.3    
38.5    
38.7    
38.8    
39.0    
39.2    
39.4    
39.6    
39.8    
40.0    
40.1    
40.3    
40.5    
40.7    
40.9    
41.1    
41.3    
41.4    
41.6    
41.8    
42.0    
42.2    
42.4    
42.6    
42.8    
42.9    
43.1    
43.3    
43.5    
43.7    
43.9    
44.1    
44.2    
44.4    
44.6    
44.8    
45.0    
45.2    
45.4    
45.5    
45.7    
45.9    
46.1    
46.3    
46.5    
46.7    
46.8    
47.0    
47.2    
47.4    
47.6    
47.8    
48.0    
48.1    
48.3    
48.5    
48.7    
48.9    
49.1    
49.3    
49.4    
49.6    
49.8    
50.0    
50.2    
50.4    
50.6    
50.7    
50.9    
51.1    
51.3    
51.5    
51.7    
51.9    
52.0    
52.2    
52.4    
52.6    
52.8    
53.0    
53.2    
53.3    
53.5    
53.7    
53.9    
54.1    
54.3    
54.5    
54.6    
54.8    
55.0    
55.2    
55.4    
55.6    
55.8    
55.9    
56.1    
56.3    
56.5    
56.7    
56.9    
57.1    
57.2    
57.4    
57.6    
57.8    
58.0    
58.2    
58.4    
58.6    
58.7    
58.9    
59.1    
59.3    
59.5    
59.7    
59.9    
60.0    
60.2    
60.4    
60.6    
60.8    
61.0    
61.2    
61.3    
61.5    
61.7    
61.9    
62.1    
62.3    
62.5    
62.6    
62.8    
63.0    
63.2    
63.4    
63.6    
63.8    
63.9    
64.1    
64.3    
64.5    
64.7    
64.9    
65.1    
65.2    
65.4    
65.6    
65.8    
66.0    
66.2    
66.4    
66.5    
66.7    
66.9    
67.1    
67.3    
67.5    
67.7    
67.8    
68.0    
68.2    
68.4    
68.6    
68.8    
69.0    
69.1    
69.3    
69.5    
69.7    
69.9    
70.1    
70.3    
70.4    
70.6    
70.8    
71.0    
71.2    
71.4    
71.6    
71.7    
71.9    
72.1    
72.3    
72.5    
72.7    
72.9    
73.0    
73.2    
73.4    
73.6    
73.8    
74.0    
74.2    
74.3    
74.5    
74.7    
74.9    
75.1    
75.3    
75.5    
75.7    
75.8    
76.0    
76.2    
76.4    
76.6    
76.8    
77.0    
77.1    
77.3    
77.5    
77.7    
77.9    
78.1    
78.3    
78.4    
78.6    
78.8    
79.0    
79.2    
79.4    
79.6    
79.7    
79.9    
80.1    
80.3    
80.5    
80.7    
80.9    
81.0    
81.2    
81.4    
81.6    
81.8    
82.0    
82.2    
82.3    
82.5    
82.7    
82.9    
83.1    
83.3    
83.5    
83.6    
83.8    
84.0    
84.2    
84.4    
84.6    
84.8    
84.9    
85.1    
85.3    
85.5    
85.7    
85.9    
86.1    
86.2    
86.4    
86.6    
86.8    
87.0    
87.2    
87.4    
87.5    
87.7    
87.9    
88.1    
88.3    
88.5    
88.7    
88.8    
89.0    
89.2    
89.4    
89.6    
89.8    
90.0    
90.1    
90.3    
90.5    
90.7    
90.9    
91.1    
91.3    
91.4    
91.6    
91.8    
92.0    
92.2    
92.4    
92.6    
92.8    
92.9    
93.1    
93.3    
93.5    
93.7    
93.9    
94.1    
94.2    
94.4    
94.6    
94.8    
95.0    
95.2    
95.4    
95.5    
95.7    
95.9    
96.1    
96.3    
96.5    
96.7    
96.8    
97.0    
97.2    
97.4    
97.6    
97.8    
98.0    
98.1    
98.3    
98.5    
98.7    
98.9    
99.1    
99.3    
99.4    
99.6    
99.8    
-found 32 predictions from path traversal
Error with prediction: GlimmerHMM lend_intergenic: 181480, rend_intergenic: 181477  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 30792, rend_intergenic: 25084  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 76420, rend_intergenic: 72809  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
Error with prediction: snap lend_intergenic: 145303, rend_intergenic: 142671  at /venv/opt/evidencemodeler-1.1.1/evidence_modeler.pl line 3218.
GOT 5prime partial gene.

-reporting highest scoring path thru the trellis.
-found 29 predictions persist after filtering for low support