zcat input_1/DRR030435_1.fastq.gz > DRR030435_1.fastq; zcat input_1/DRR030435_2.fastq.gz > DRR030435_2.fastq; singularity exec -B /suikou/files/m64g/user2/work/101:/mnt --pwd /mnt /home/user2/img/c2997108/centos7:1-trinity_2.8.5-kallisto_0.46.0-blast_2.9.0-trinotate-3.1.1-R_4-kegg_4 prinseq-lite.pl -fastq DRR030435_1.fastq -fastq2 DRR030435_2.fastq -out_good output.prinseq/DRR030435_1.fastq -out_bad null -out_format 3 -trim_tail_right 5 -trim_qual_right 20 -ns_max_p 20 -min_len 30 -trim_qual_window 5; mv output.prinseq/DRR030435_1.fastq_1.fastq output.prinseq/DRR030435_1.fastq; mv output.prinseq/DRR030435_1.fastq_2.fastq output.prinseq/DRR030435_2.fastq;rm -f DRR030435_1.fastq DRR030435_2.fastq
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
perl: warning: Setting locale failed.
perl: warning: Please check that your locale settings:
	LANGUAGE = (unset),
	LC_ALL = (unset),
	LANG = "ja_JP.UTF-8"
    are supported and installed on your system.
perl: warning: Falling back to the standard locale ("C").
Input and filter stats:
	Input sequences (file 1): 100,000
	Input bases (file 1): 10,000,000
	Input mean length (file 1): 100.00
	Input sequences (file 2): 100,000
	Input bases (file 2): 10,000,000
	Input mean length (file 2): 100.00
	Good sequences (pairs): 97,556
	Good bases (pairs): 19,511,200
	Good mean length (pairs): 200.00
	Good sequences (singletons file 1): 2,076 (2.08%)
	Good bases (singletons file 1): 207,600
	Good mean length (singletons file 1): 100.00
	Good sequences (singletons file 2): 293 (0.29%)
	Good bases (singletons file 2): 29,300
	Good mean length (singletons file 2): 100.00
	Bad sequences (file 1): 368 (0.37%)
	Bad bases (file 1): 36,800
	Bad mean length (file 1): 100.00
	Bad sequences (file 2): 2,076 (2.08%)
	Bad bases (file 2): 207,600
	Bad mean length (file 2): 100.00
	Sequences filtered by specified parameters:
	trim_qual_right: 1307
	trim_tail_right: 63
	min_len: 1149