pp Comparative-genomics~FastANI -c 8 -m 32 input_1/xr1.hap1chr1-2.fa input_2/xr1.hap2chr1-2.fa PID: 2643171 /home/yoshitake.kazutoshi/files/m256y/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/pp 'Comparative-genomics~FastANI' -c 8 -m 32 input_1/xr1.hap1chr1-2.fa input_2/xr1.hap2chr1-2.fa Checking the realpath of input files. 1 script: /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI Containers: biocontainers/samtools:v1.7.0_cv4 c2997108/centos7:metacor7 centos:centos6 quay.io/biocontainers/fastani:1.34--h4dfc31f_4 using docker ++ set -o pipefail + set -eux + set -o pipefail ++ echo input_1/xr1.hap1chr1-2.fa ++ grep '[.]gz$' ++ wc -l ++ true + '[' 0 = 1 ']' ++ echo input_2/xr1.hap2chr1-2.fa ++ grep '[.]gz$' ++ wc -l ++ true + '[' 0 = 1 ']' ++ basename input_1/xr1.hap1chr1-2.fa ++ basename input_2/xr1.hap2chr1-2.fa + FUNC_RUN_DOCKER quay.io/biocontainers/fastani:1.34--h4dfc31f_4 fastANI -q input_1/xr1.hap1chr1-2.fa -r input_2/xr1.hap2chr1-2.fa -o fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt --visualize + PP_RUN_IMAGE=quay.io/biocontainers/fastani:1.34--h4dfc31f_4 + shift + PP_RUN_DOCKER_CMD=("${@}") ++ date +%Y%m%d_%H%M%S_%3N + PPDOCNAME=pp20250303_003723_132_26838 + echo pp20250303_003723_132_26838 ++ id -u ++ id -g + docker run --name pp20250303_003723_132_26838 -v /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI:/suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -w /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -v /suikou/files/m256y/yoshitake.kazutoshi:/suikou/files/m256y/yoshitake.kazutoshi -u 2007:600 -i --rm quay.io/biocontainers/fastani:1.34--h4dfc31f_4 fastANI -q input_1/xr1.hap1chr1-2.fa -r input_2/xr1.hap2chr1-2.fa -o fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt --visualize >>>>>>>>>>>>>>>>>> Reference = [input_2/xr1.hap2chr1-2.fa] Query = [input_1/xr1.hap1chr1-2.fa] Kmer size = 16 Fragment length = 3000 Threads = 1 ANI output file = fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt Sanity Check = 0 >>>>>>>>>>>>>>>>>> INFO [thread 0], skch::main, Count of threads executing parallel_for : 1 INFO [thread 0], skch::Sketch::build, window size for minimizer sampling = 24 INFO [thread 0], skch::Sketch::build, minimizers picked from reference = 909883 INFO [thread 0], skch::Sketch::index, unique minimizers = 773785 INFO [thread 0], skch::Sketch::computeFreqHist, Frequency histogram of minimizers = (1, 734039) ... (186, 1) INFO [thread 0], skch::Sketch::computeFreqHist, consider all minimizers during lookup. INFO [thread 0], skch::main, Time spent sketching the reference : 1.20904 sec INFO [thread 0], skch::main, Start Map 1 INFO [thread 0], skch::main, Time spent mapping fragments in query #1 : 21.8703 sec INFO [thread 0], skch::main, Time spent post mapping : 0.0148263 sec INFO [thread 0], skch::main, ready to exit the loop INFO, skch::main, parallel_for execution finished + mkdir -p reformat + DO_EXONERATE fastareformat input_1/xr1.hap1chr1-2.fa + sed 's/\r//g' /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI: 行 33: DO_EXONERATE: コマンドが見つかりません ++ onerror 33 ++ status=127 ++ script=/suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI ++ line=33 ++ shift ++ set +x ------------------------------------------------------------ Error occured on /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI [Line 33]: Status 127 PID: 2643177 User: yoshitake.kazutoshi Current directory: /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI Command line: /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI ------------------------------------------------------------ PID: 2658529 /home/yoshitake.kazutoshi/files/m256y/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/pp 'Comparative-genomics~FastANI' -c 8 -m 32 input_1/xr1.hap1chr1-2.fa input_2/xr1.hap2chr1-2.fa Checking the realpath of input files. 1 script: /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/PortablePipeline/PortablePipeline/scripts/Comparative-genomics~FastANI Containers: c2997108/centos7:metacor7 c2997108/ubuntu20:R-2_gplots_genoPlotR_2 centos:centos6 quay.io/biocontainers/fastani:1.34--h4dfc31f_4 using docker ++ set -o pipefail + set -eux + set -o pipefail ++ echo input_1/xr1.hap1chr1-2.fa ++ grep '[.]gz$' ++ wc -l ++ true + '[' 0 = 1 ']' ++ echo input_2/xr1.hap2chr1-2.fa ++ grep '[.]gz$' ++ wc -l ++ true + '[' 0 = 1 ']' ++ basename input_1/xr1.hap1chr1-2.fa ++ basename input_2/xr1.hap2chr1-2.fa + FUNC_RUN_DOCKER quay.io/biocontainers/fastani:1.34--h4dfc31f_4 fastANI -t 8 -q input_1/xr1.hap1chr1-2.fa -r input_2/xr1.hap2chr1-2.fa -o fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt --visualize + PP_RUN_IMAGE=quay.io/biocontainers/fastani:1.34--h4dfc31f_4 + shift + PP_RUN_DOCKER_CMD=("${@}") ++ date +%Y%m%d_%H%M%S_%3N + PPDOCNAME=pp20250303_005201_081_11437 + echo pp20250303_005201_081_11437 ++ id -u ++ id -g + docker run --name pp20250303_005201_081_11437 -v /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI:/suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -w /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -v /suikou/files/m256y/yoshitake.kazutoshi:/suikou/files/m256y/yoshitake.kazutoshi -u 2007:600 -i --rm quay.io/biocontainers/fastani:1.34--h4dfc31f_4 fastANI -t 8 -q input_1/xr1.hap1chr1-2.fa -r input_2/xr1.hap2chr1-2.fa -o fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt --visualize >>>>>>>>>>>>>>>>>> Reference = [input_2/xr1.hap2chr1-2.fa] Query = [input_1/xr1.hap1chr1-2.fa] Kmer size = 16 Fragment length = 3000 Threads = 8 ANI output file = fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt Sanity Check = 0 >>>>>>>>>>>>>>>>>> INFO [thread 0], skch::main, Count of threads executing parallel_for : 8 INFO [thread 0], skch::Sketch::build, window size for minimizer sampling = 24 INFO [thread 4], skch::main, ready to exit the loop INFO [thread 0], skch::Sketch::build, minimizers picked from reference = 909883 INFO [thread 3], skch::main, ready to exit the loop INFO [thread 2], skch::main, ready to exit the loop INFO [thread 5], skch::main, ready to exit the loop INFO [thread 6], skch::main, ready to exit the loop INFO [thread 1], skch::main, ready to exit the loop INFO [thread 7], skch::main, ready to exit the loop INFO [thread 0], skch::Sketch::index, unique minimizers = 773785 INFO [thread 0], skch::Sketch::computeFreqHist, Frequency histogram of minimizers = (1, 734039) ... (186, 1) INFO [thread 0], skch::Sketch::computeFreqHist, consider all minimizers during lookup. INFO [thread 0], skch::main, Time spent sketching the reference : 1.52038 sec INFO [thread 0], skch::main, Start Map 1 INFO [thread 0], skch::main, Time spent mapping fragments in query #1 : 22.4838 sec INFO [thread 0], skch::main, Time spent post mapping : 0.0140316 sec INFO [thread 0], skch::main, ready to exit the loop INFO, skch::main, parallel_for execution finished ++ basename input_1/xr1.hap1chr1-2.fa ++ basename input_2/xr1.hap2chr1-2.fa + FUNC_RUN_DOCKER c2997108/ubuntu20:R-2_gplots_genoPlotR_2 Rscript /usr/local/FastANI/scripts/visualize.R input_1/xr1.hap1chr1-2.fa input_2/xr1.hap2chr1-2.fa fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt.visual + PP_RUN_IMAGE=c2997108/ubuntu20:R-2_gplots_genoPlotR_2 + shift + PP_RUN_DOCKER_CMD=("${@}") ++ date +%Y%m%d_%H%M%S_%3N + PPDOCNAME=pp20250303_005226_504_21727 + echo pp20250303_005226_504_21727 ++ id -u ++ id -g + docker run --name pp20250303_005226_504_21727 -v /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI:/suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -w /suikou/files/m256y/yoshitake.kazutoshi/work/pp-dev/yoshitake/test/Comparative-genomics~FastANI -v /suikou/files/m256y/yoshitake.kazutoshi:/suikou/files/m256y/yoshitake.kazutoshi -u 2007:600 -i --rm c2997108/ubuntu20:R-2_gplots_genoPlotR_2 Rscript /usr/local/FastANI/scripts/visualize.R input_1/xr1.hap1chr1-2.fa input_2/xr1.hap2chr1-2.fa fastANI.xr1.hap1chr1-2.fa.xr1.hap2chr1-2.fa.txt.visual Loading required package: ade4 Loading required package: grid null device 1 + post_processing + '[' 1 = 1 ']' + rm -f /home/yoshitake.kazutoshi/files/m256y/pp-dev/yoshitake/test/Comparative-genomics~FastANI/pp-singularity-flag + '[' '' = y ']' + echo 0 + exit